艾草多糖结构特征及抗氧化活性研究

王子朝; 舒志涵; 郑毅; 陈庆功

doi:10.12187/2026.05.002

October 2026

Article Contents

Wang Zichao, Shu Zhihan, Zheng Yi and et al. Structural characterization and antioxidant activity of Artemisia argyi polysaccharids[J]. Journal of Light Industry, 2026, 41(5): 13-24. doi: 10.12187/2026.05.002

Citation: Wang Zichao, Shu Zhihan, Zheng Yi and et al. Structural characterization and antioxidant activity of Artemisia argyi polysaccharids[J]. Journal of Light Industry, 2026, 41(5): 13-24. doi: 10.12187/2026.05.002 shu

Structural characterization and antioxidant activity of Artemisia argyi polysaccharids

1. School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China;
2. Jiaozuo Haoshida Agriculture Co., Ltd., Jiaozuo 454950, China

Received Date: 2024-08-07
Accepted Date: 2026-09-29

Abstract

【Objective】This study aimed to analyze the structural characteristics and antioxidant activity of Artemisia argyi polysaccharides, thereby facilitating the high-value utilization of Artemisia argyi resources and the development of natural antioxidant functional ingredients.【Methods】Artemisia argyi polysaccharides were extracted by water extraction and ethanol precipitation. High-performance liquid chromatography and spectroscopic analysis were used to analyze their composition and structural characteristics. The in vitro antioxidant activity was evaluated by DPPH, superoxide anion, hydroxyl, and ABTS⁺ radical scavenging assays. The cellular oxidative damage repair effect was assessed using an H₂O₂-induced oxidative damage model in the murine monocyte/macrophage cell line RAW264.7.【Results】The polysaccharides consisted of glucose, galactose, arabinose, mannose, xylose, and rhamnose at a molar ratio of 1.00∶0.26∶0.23∶0.19∶0.15∶0.13. Their weight-average molecular weight and polydispersity index were 25 934 Da and 1.211, respectively. At mass concentrations of 0.31~5.00 mg/mL, the scavenging capacities of the polysaccharides against DPPH, superoxide anion, hydroxyl, and ABTS⁺ radicals were concentration-dependent. The maximum ABTS⁺radical scavenging rate reached 99.68%, which was comparable to that of ascorbic acid (VC). The polysaccharides showed no obvious cytotoxicity toward RAW264.7 cells. In the H₂O₂-induced oxidative damage model, the polysaccharides increased T-SOD, CAT, and GSH-Px activities from 0.17 U/mg protein, 1.21 U/mg protein, and 4.90 U/mg protein to 0.33 U/mg protein, 1.58 U/mg protein, and 8.88 U/mg protein, respectively, and decreased the MDA level from 15.92 nmol/mL to 8.89 nmol/mL in a concentration-dependent manner.【Conclusion】The obtained Artemisia argyi polysaccharides exhibited significant in vitro and intracellular antioxidant activity, exerting oxidative repair effects by enhancing antioxidant enzyme activities and reducing lipid peroxidation, thus indicating their potential for development as natural antioxidants.
- Artemisia argyi polysaccharide,
- structural characterization,
- antioxidant activity,
- cellular oxidation repair effect
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Structural characterization and antioxidant activity of Artemisia argyi polysaccharids

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