海藻酸钠-羧甲基木薯淀粉复合凝胶珠制备及其性能评价

张义平; 赵紫梦; 钱亚睿; 曾钰琪; 赵丽丽; 陈红; 姜晓冰

doi:10.12187/2026.05.004

October 2026

Article Contents

Zhang Yiping, Zhao Zimeng, Qian Yarui, et al. Preparation and performance evaluation of sodium alginate-carboxymethyl cassava starch composite gel beads[J]. Journal of Light Industry, 2026, 41(5): 37-49. doi: 10.12187/2026.05.004

Citation: Zhang Yiping, Zhao Zimeng, Qian Yarui, et al. Preparation and performance evaluation of sodium alginate-carboxymethyl cassava starch composite gel beads[J]. Journal of Light Industry, 2026, 41(5): 37-49. doi: 10.12187/2026.05.004 shu

Preparation and performance evaluation of sodium alginate-carboxymethyl cassava starch composite gel beads

Zhang Yiping ^1,2 ,
Zhao Zimeng ^1,2 ,
Qian Yarui ^1,2 ,
Zeng Yuqi ^1,2 ,
Zhao Lili ^1,2 ,
Chen Hong ^1,2 ,
Jiang Xiaobing ^1,2,,

1. College of Life Sciences, Henan Normal University, Xinxiang 453007, China;
2. Henan Engineering Laboratory for Green Transformation Technology of Functional Microorganisms, Xinxiang 453007, China

Corresponding author: Jiang Xiaobing, jxb841001@163.com
Received Date: 2025-09-22
Accepted Date: 2026-01-15

Abstract

【Objective】This study aimed to improve the pH stability and oral bioavailability of probiotics.【Methods】In this study, Lactobacillus plantarum ZP-6 (LP ZP-6) was used as a representative probiotic, and sodium alginate (SA) and carboxymethyl cassava starch (CMS) were used as carrier materials to prepare SA-CMS composite gel beads efficiently embedding LP ZP-6 by the complex coacervation method. Single-factor and orthogonal experiments were conducted to optimize the embedding conditions of the SA-CMS composite gel beads, and the effects of the gel bead structure on the pH stability and in vitro survival rate of LP ZP-6 in simulated digestive fluids were investigated.【Results】Compared with SA gel beads, SA-CMS composite gel beads significantly improved the embedding rate of LP ZP-6, the survival rate of LP ZP-6 under different pH conditions, and the anti-swelling property of the gel beads. The highest embedding rate of LP ZP-6 by the SA-CMS composite gel beads (89.45%) was obtained when the concentrations of CMS, SA, and calcium chloride were 25 mg/mL, 20 mg/mL, and 45 mg/mL, respectively. These gel beads significantly enhanced the pH stability and oral bioavailability of LP ZP-6, with the survival rate of LP ZP-6 remaining above 80% after simulated digestion in vitro.【Conclusion】The SA-CMS composite gel beads can serve as an effective carrier for probiotics to enhance their pH stability and oral bioavailability.
- Lactobacillus plantarum,
- carboxymethyl cassava starch,
- sodium alginate,
- gel bead,
- pH stability,
- oral bioavailability
References
1. [1]
  Thinkohkaew K,Jonjaroen V,Niamsiri N,et al.Microencapsulation of probiotics in chitosan-coated alginate/gellan gum:optimization for viability and stability enhancement[J].Food Hydrocolloids,2024,151:109788.
2. [2]
  Thinkohkaew K,Aumphaiphensiri N,Tangamornsiri T,et al.Inulin extracted from burdock root (Arctium lappa L.)incorporated alginate/chitosan hydrogel beads for probiotics encapsulation[J].Journal of Agriculture and Food Research,2024,18:101405.
3. [3]
  Mohammad N A,Zaidel D N A,Muhamad I I,et al.Biopolymeric encapsulation of probiotics for improved release properties in the gastrointestinal digestion system[J].IOP Conference Series:Materials Science and Engin-eering,2020,778(1):012033.
4. [4]
  Liu H,Gong J,Chabot D,et al.Improved survival of Lactobacillus zeae LB1 in a spray dried alginate-protein matrix[J].Food Hydrocolloids,2018,78:100-108.
5. [5]
  Huang Y Y,Yao Q B,Jia X Z,et al.Characterization and application in yogurt of genipin-crosslinked chitosan microcapsules encapsulating with Lactiplantibacillus plantarum DMDL 9010[J].International Journal of Biological Macromolecules,2023,248:125871.
6. [6]
  Hu X X,Liu C Y,Zhang H T,et al.In vitro digestion of sodium alginate/pectin co-encapsulated Lactobacillus bulgaricus and its application in yogurt bilayer beads[J].International Journal of Biological Macromolecules,2021,193(Pt B):1050-1058.
7. [7]
  Zhong D N,Zhang D X,Chen W,et al.Orally deliverable strategy based on microalgal biomass for intestinal disease treatment[J].Science Advances,2021,7(48):eabi9265.
8. [8]
  Cheng C,Sun M M,Wang L,et al.Zein and soy polysaccharide encapsulation enhances probiotic viability and modulates gut microbiota[J].LWT-Food Science and Technology,2024,210:116827.
9. [9]
  Zheng D W,Li R Q,An J X,et al.Prebiotics-encapsulated probiotic spores regulate gut microbiota and suppress colon cancer[J].Advanced Materials,2020,32(45):2004529.
10. [10]
  Deng Z Y,Wang S S,Zhou B,et al.Carboxy-methylpachymaran-zein coated plant microcapsules-based β-galactosidase encapsulation system for long-term effective delivery[J].Food Research International,2020,128:108867.
11. [11]
  He X D,Zeng L Y,Cheng X P,et al.Shape memory composite hydrogel based on sodium alginate dual crosslinked network with carboxymethyl cellulose[J].European Polymer Journal,2021,156:110592.
12. [12]
  Phansroy N,Boonyod S,Mulasake O,et al.Innovative environment-friendly liquid fertilizer bead from sodium alginate coating with IPN membrane derived from natural rubber and cassava starch[J].Journal of Polymer Research,2024,31(3):67.
13. [13]
  彭爽燨.负载咖啡酸@二氧化硅纳米粒子的木薯淀粉/羧甲基纤维素钠可食用膜的制备与表征及其在肉品保鲜中的应用[D].镇江:江苏大学,2023. Peng S X.Preparation and cheracterizaton of cassava starch/sodium carboxymethyl cellulose edible film loaded with caffic acid@silica nanoparticles and the application in meat preservation[D].Zhenjiang:Jiangsu University,2023.
14. [14]
  梅既强.木薯淀粉的化学改性及其衍生物的结构、性质和体外消化率的研究[D].合肥:合肥工业大学,2016. Mei J Q.Studies on chemical modification of cassava starch and structure,properties and in vitro digestibility of its derivatives[D].Hefei:Hefei University of Technology,2016.
15. [15]
  Tavares K M,De Campos A,Mitsuyuki M C,et al.Corn and cassava starch with carboxymethyl cellulose films and its mechanical and hydrophobic properties[J].Carbohydrate Polymers,2019,223:115055.
16. [16]
  陈渊,杨家添,谢秋季,等.木薯羧甲基淀粉的机械活化固相化学法制备、表征及其特性[J].食品科学,2018,39(2):45-52.
  Chen Y,Yang J T,Xie Q J,et al.Preparation,structural characterization and properties of carboxymethyl cassava starch by mechanical activation-assisted solid-state reaction[J].Food Science,2018,39(2):45-52.
17. [17]
  Zhang Y P,Liu K,Chi C D,et al.Preparation,stability and controlled release properties of starch-based micelles for oral delivery of hydrophobic bioactive molecules[J].International Journal of Biological Macromolecules,2024,262:130241.
18. [18]
  Coelho-Rocha N D,De Castro C P,De Jesus L C L,et al.Microencapsulation of lactic acid bacteria improves the gastrointestinal delivery and in situ expression of recombinant fluorescent protein[J].Frontiers in Microbiology,2018,9:2398.
19. [19]
  Yan D D,Pan Y F,Cai P X,et al.Redox/pH dual-responsive sodium alginate/cassava starch composite hydrogel beads for slow release of insecticides[J].Reactive and Functional Polymers,2025,215:106389.
20. [20]
  Deng Z Y,Li J,Song R,et al.Carboxymethylpachy-maran/alginate gel entrapping of natural pollen capsules for the encapsulation,protection and delivery of probiotics with enhanced viability[J].Food Hydrocolloids,2021,120:106855.
21. [21]
  Li H. Modeling of stimuli-responsive hydrogels:a transient analysis[M].//Li H, Silberschmidt V. The mechanics of hydrogels. Cambridge: Woodhead Publishing,2022:223-268.
22. [22]
  焦文娟.脂肪晶体结构对脂肪结晶乳液消化性能的影响及机理研究[D].广州:华南理工大学,2020. Jiao W J.The effect of fat crystal structure on the digestibility of fat crystalline emulsion and its mechanism[D].Guangzhou:South China University of Technology,2020.
23. [23]
  Yu H,Kong Q,Wang M R,et al.Improved viability of probiotics by encapsulation in chickpea protein matrix during simulated gastrointestinal digestion by succinylated modification[J].International Journal of Biological Macromolecules,2024,260:129614.
24. [24]
  Teobaldi A G, Parra E J C,Barrera G N,et al.Effect of food proteins on wheat starch pasting and thermal properties[J].Foods,2025,14(22):3865.
25. [25]
  袁怀波,刘文宏,樊良.交联羧甲基木薯淀粉吸附Ca²⁺的研究[J].食品科学,2009,30(5):96-99.
  Yuan H B,Liu W H,Fan L.Study on adsorption of cross-linked carboxymethyl cassava starch to Ca²⁺from aqueous solution[J].Food Science,2009,30(5):96-99.
26. [26]
  Tiamwong S,Yukhajon P,Noisong P,et al.Eco-friendly starch composite supramolecular alginate-Ca²⁺ hydrogel as controlled-release P fertilizer with low responsiveness to multiple environmental stimuli[J].Gels,2023,9(3):204.
27. [27]
  Zheng B D,Yu Y Z,Yuan X L,et al.Sodium alginate/carboxymethyl starch/κ-carrageenan enteric soft capsule:processing,characterization,and rupture time evaluation[J].International Journal of Biological Macromolecules,2023,244:125427.
28. [28]
  Fang K,Li P,Huang X R,et al.pH/NIR light-responsive carboxymethyl starch/sodium alginate/MXene hydrogel beads for doxorubicin delivery[J].International Journal of Biological Macromolecules,2025,321(Pt 2):146306.
29. [29]
  Zhang Y L,Xie S M,Huang W J,et al.Fabrication and characterization of complex coacervates utilizing gelatin and carboxymethyl starch[J].Journal of the Science of Food and Agriculture,2024,104(6):3585-3593.
30. [30]
  Blemur L,Le T C,Marcocci L,et al.Carboxymethyl starch/alginate microspheres containing diamine oxidase for intestinal targeting[J].Biotechnology and Applied Biochemistry,2016,63(3):344-353.
31. [31]
  Pooresmaeil M,Namazi H.Development of the new pH-driven carrier from alginate/carboxymethyl starch bio-coated co-drugs@COF-OH for controlled and concomitant colon cancer treatment[J].International Journal of Biological Macromolecules,2023,239:124322.
32. [32]
  Li K N,Wang S J,Liu W J,et al.Comparative genomic analysis of 455 Lactiplantibacillus plantarum isolates:Habitat-specific genomes shaped by frequent recombination[J].Food Microbiology,2022,104:103989.
33. [33]
  Hua S B,Ma H Z,Li X,et al.pH-sensitive sodium alginate/poly(vinyl alcohol)hydrogel beads prepared by combined Ca²⁺ crosslinking and freeze-thawing cycles for controlled release of diclofenac sodium[J].International Journal of Biological Macromolecules,2010,46(5):517-523.
34. [34]
  Liu L H,Gu P,Li C J,et al.Improve the quality of bog bilberry juice by controlling the inoculation pH and timing of Lactobacillus plantarum[J].Journal of Food Processing and Preservation,2021,45(9):e15541.
35. [35]
  Xin W G,Jiang Y H,Zhao Y T,et al.Encapsulation of Lactiplantibacillus plantarum in a sodium alginate/pectin/tea polyphenol complex:enhancing stability and bioactivity for fermented milk products[J].Food Chemistry,2025,488:144851.
36. [36]
  Lin Y H,Xu J C,Gao X.Development of antioxidant sodium alginate gel beads encapsulating curcumin/gum Arabic/gelatin microcapsules[J].Food Hydrocolloids,2024,152:109901.
37. [37]
  Wang M M,Zang Y P,Hong K J,et al.Preparation of pH-sensitive carboxymethyl cellulose/chitosan/alginate hydrogel beads with reticulated shell structure to deliver Bacillus subtilis natto[J].International Journal of Biological Macromolecules,2021,192:684-691.
38. [38]
  Wang W,Liu W,Wu J S,et al.Preparation and characterization of particle-filled microgels by chemical cross-linking based on zein and carboxymethyl starch for delivering the quercetin[J].Carbohydrate Polymers,2024,323:121375.
39. [39]
  Muthumari B,Kumar B V,Kavitha M,et al.Optimization of sodium alginate-galactoxyloglucan blended hydrogel beads through ionotropic gelation method[J].International Journal of Biological Macromolecules,2023,242(Pt 1):124630.
40. [40]
  Li W,Liu L M,Tian H F,et al.Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability[J].Carbohydrate Polymers,2019,223:115065.
41. [41]
  Jin H N,Wang L,Yang S Y,et al.Producing mixed-soy protein adsorption layers on alginate microgels to controlled-release β-carotene[J].Food Research Inter-national,2023,164:112319.
42. [42]
  Chai W H,An Y L,Wang X L,et al.Optimization of SA-gel hydrogel printing parameters for extrusion-based 3D bioprinting[J].Gels,2025,11(7):552.
43. [43]
  Ping M K X,Zhi H W,Aziz N S,et al.Optimization of agarose-alginate hydrogel bead components for encapsula-tion and transportation of stem cells[J].Journal of Taibah University Medical Sciences,2023,18(1):104-116.
44. [44]
  Li Y H,Liu G W,Liao N,et al.Construction of walnut protein/tea polyphenol/alginate complex for enhancing heat and gastrointestinal tolerance of lactic acid bacteria[J].Food Hydrocolloids,2024,149:109523.
45. [45]
  Conway P L,Gorbach S L,Goldin B R.Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells[J].Journal of Dairy Science,1987,70(1):1-12.
46. [46]
  Rao Y,Deng J,Zhang C Y,et al.Probiotics encapsulated by calcium pectin/chitosan-calcium pectin/sodium alginate-pectin-whey through biofilm-based microencapsulation strategy and their preventive effects on ulcerative colitis[J].Food Hydrocolloids,2025,158:110501.
47. [47]
  Fu Y X,Liu L,Zhang J H,et al.Reinforcing alginate matrixes by tea polysaccharide conjugates or their stabilized nanoemulsion for probiotics encapsulation:characterization,survival after gastrointestinal digestion and ambient storage[J].International Journal of Biological Macromolecules,2023,253(Pt 3):126828.
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Preparation and performance evaluation of sodium alginate-carboxymethyl cassava starch composite gel beads

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