JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 41 Issue 5
October 2026
Article Contents
Shi Yixin, Zhou Xueyan, Wang Qi and et al. Construction of the Y1Ste20 gene knockout recombinant plasmid in Chaetomium globosum CGMCC 6882 and analysis of its effects on mycelial morphology[J]. Journal of Light Industry, 2026, 41(5): 85-93. doi: 10.12187/2026.05.008
Citation: Shi Yixin, Zhou Xueyan, Wang Qi and et al. Construction of the Y1Ste20 gene knockout recombinant plasmid in Chaetomium globosum CGMCC 6882 and analysis of its effects on mycelial morphology[J]. Journal of Light Industry, 2026, 41(5): 85-93. doi: 10.12187/2026.05.008 shu

Construction of the Y1Ste20 gene knockout recombinant plasmid in Chaetomium globosum CGMCC 6882 and analysis of its effects on mycelial morphology

  • Corresponding author: Wang Zichao, 6832917@163.com
  • Received Date: 2026-01-27
    Accepted Date: 2026-04-24
  • 【Objective】This study aimed to investigate the regulatory role of the Ste20 kinase homolog gene from Yarrowia lipolytica(Y1Ste20) in Chaetomium globosum CGMCC 6882 after targeted gene knockout, with particular focus on its effects on colony diameter and hyphal morphology.【Methods】A Y1Ste20 gene knockout recombinant plasmid was constructed using CRISPR-Cas9 gene editing technology. The recombinant plasmid was introduced into C.globosum CGMCC 6882 protoplasts via PEG-mediated protoplast transformation to obtain the Y1Ste20 gene knockout strain C.globosum XY1104, and its colony growth characteristics and hyphal morphology were analyzed.【Results】The Y1Ste20 gene knockout recombinant plasmid (pFC332-5S rRNA-Ste20 gRNA) was constructed using CRISPR-Cas9 gene editing technology and successfully transferred into C.globosum CGMCC 6882 protoplasts, yielding the Y1Ste20 gene knockout strain C.globosum XY1104. On solid medium, the colony diameter of strain XY1104 was (201.89±9.35) mm, which was smaller than that of C.globosum CGMCC 6882 ((434.35±10.03) mm). In liquid culture, strain XY1104 exhibited fine, short, rod-like hyphae without entanglement, whereas C.globosum CGMCC 6882 lacked discrete hyphae and readily formed mycelial pellets.【Conclusion】Disruption of the Y1Ste20 gene led to a reduced colony diameter and hyphae that were fine, short, and rod-like, grew densely, and showed reduced entanglement in C.globosum CGMCC 6882. These findings indicate that Y1Ste20 is involved in regulating hyphal growth and morphogenesis in C.globosum CGMCC 6882.
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