CHENG Chuan-ling, SONG Hui, WANG Wen-liang and et al. The effect of mobile phase and flow rate on the qualitative analysis of fructosazine with high performance liquid chromatography[J]. Journal of Light Industry, 2012, 27(4): 65-68. doi: 10.3969/j.issn.1004-1478.2012.04.017
Citation:
CHENG Chuan-ling, SONG Hui, WANG Wen-liang and et al. The effect of mobile phase and flow rate on the qualitative analysis of fructosazine with high performance liquid chromatography[J]. Journal of Light Industry, 2012, 27(4): 65-68.
doi:
10.3969/j.issn.1004-1478.2012.04.017
The effect of mobile phase and flow rate on the qualitative analysis of fructosazine with high performance liquid chromatography
-
College of Food and Bioeng., Zhengzhou Univ. of Light Ind., Zhengzhou 450002, China;
-
Honghe Cigarette Factory, Hongyun Honghe Tobacco(Group) Co., Ltd., Honghe 652300, China
-
Received Date:
2011-12-21
Available Online:
2012-07-15
-
Abstract
The effect of mobile phase and flow rate on the qualitative analysis of fructosazine with high performance liquid chromatography was investigated.The results showed that the effect of qualitative analysis was the best using acetonitrile and water as mobile phase (80:20,V/V)and flow rate 1.0 mL/min under condition as follows:Water Carbohydrate Analysis works as separation column,detection wavelength is 275 nm,and the volume of injection is 10μL.
-
-
References
-
[1]
卢斌斌,李鹏,谢剑平,等.烟草及烟草制品中脱氧果糖嗪含量分析[J].香料香精化妆品,2009, 3(6):5.
-
[2]
李琦,叶韵华,闫爱新,等.三七中2-(1',2',3',4'-四羟基丁基)-6-(2",3",4"-三羟基丁基)-吡嗪的分离、鉴定及药理活性研究[J].高等学校化学学报,2001,22(11):1824.
-
[3]
秦文娟,马立斌.掌叶半夏化学成分的研究[J].中草药,1995,26(1):3.
-
[4]
Fujii S,Kikuchia R,Kushida H.Formation of fructosazine[J].J of Organic Chem,1966,31(7):2239.
-
[5]
樊瑛,孟昭宇,陆舍铭,等.固相萃取一高效液相色谱法测定烟草中的两种多羟基吡嗪异构体[J].分析试验室,2007,26(12):35-38.
-
[6]
黎艳玲,杨华武,周宇,等.硅烷化衍生-气相色谱/质谱法分析糖氨反应中的2,6-脱氧果糖嗪[J].色谱,2006,24(2):212.
-
Proportional views
-
-