牛排菇SB65菌株液体培养基和培养条件的优化

何培新; 秦慧迪; 安正方; 李政伟

doi:10.3969/j.issn.2095-476X.2013.01.003

January 2013

Article Contents

HE Pei-xin, QIN Hui-di, AN Zheng-fang and et al. Optimization of fermentation liquid medium and culture conditions of portabella mushroom(Agaricus brunnescens) SB65 strain[J]. Journal of Light Industry, 2013, 28(1): 11-15. doi: 10.3969/j.issn.2095-476X.2013.01.003

Citation: HE Pei-xin, QIN Hui-di, AN Zheng-fang and et al. Optimization of fermentation liquid medium and culture conditions of portabella mushroom(Agaricus brunnescens) SB65 strain[J]. Journal of Light Industry, 2013, 28(1): 11-15. doi: 10.3969/j.issn.2095-476X.2013.01.003 shu

Optimization of fermentation liquid medium and culture conditions of portabella mushroom(Agaricus brunnescens) SB65 strain

School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
Luoyang Aojite Mushroom Development Co., Ltd., Luoyang 471022, China

Received Date: 2012-11-15
Available Online: 2013-01-15

Abstract

The liquid medium and culture conditions of portabella mushroom(Agaricus brunnescens) SB65 strain were optimized by single-factor experiments and uniform design.The optimized medium were as follows(w/v):sucrose 5.5%,yeast extract 1%,MgSO₄·7H₂O 0.05%,KH₂PO₄ 1%,NaCl 0.714 7%,CaCl₂·2H₂O 1% and the optimized culture conditions were as follows:initial pH=7.2,culture temperature 28℃,culture period 12 d,rotation rate 160 r/min,medium volume 50 mL per 250 mL flask and seed volume 8% (v/v).Under the optimized medium and culture conditions,the biomass of tested strain was 4.579 g/L.
- portabella mushroom (Agaricus brunnescens),
- liquid spawn medium,
- biomass,
- culture condition
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沈阳化工大学材料科学与工程学院沈阳 110142

Optimization of fermentation liquid medium and culture conditions of portabella mushroom(Agaricus brunnescens) SB65 strain

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通讯作者: 陈斌, bchen63@163.com