JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 28 Issue 2
March 2013
Article Contents
WANG Nan and WANG Wei. Study on an extraction method of pathogenic fungal DNA from farmland soil[J]. Journal of Light Industry, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013
Citation: WANG Nan and WANG Wei. Study on an extraction method of pathogenic fungal DNA from farmland soil[J]. Journal of Light Industry, 2013, 28(2): 54-58,63. doi: 10.3969/j.issn.2095-476X.2013.02.013 shu

Study on an extraction method of pathogenic fungal DNA from farmland soil

  • Corresponding author: WANG Wei, 
  • Received Date: 2013-03-05
    Available Online: 2013-03-15
  • By comparing grinding method, washing method, cleavage and precipitation method, a method was gotten suitable for farmland fungi DNA extraction. The results showed that the quality of soil microorganism DNA is of the best which is obtained by grinding with quartz sand, washing with TENP and PBS, lysing with SDS in high-salt buffer and precipitating with isopropanol and so on. The pathogenic fungal DNA can be directly extracted from soil by using the method, which is particularly suitable for the soil pathogenic fungal extraction. The DNA obtained can be directly used for restriction enzyme digestion and PCR amplification. The method is efficient, inexpensive, easy to operate, which is of important value for the soil pathogens ecological research.
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