WANG Nan, ZHANG Gai-hong and WANG Wei. Study on molecular diagnosis of cucumber bacterial wilt[J]. Journal of Light Industry, 2013, 28(5): 21-25. doi: 10.3969/j.issn.2095-476X.2013.05.005
Citation:
WANG Nan, ZHANG Gai-hong and WANG Wei. Study on molecular diagnosis of cucumber bacterial wilt[J]. Journal of Light Industry, 2013, 28(5): 21-25.
doi:
10.3969/j.issn.2095-476X.2013.05.005
Study on molecular diagnosis of cucumber bacterial wilt
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College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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College of Bioengineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
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Corresponding author:
WANG Wei,
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Received Date:
2013-03-18
Available Online:
2013-09-15
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Abstract
The pathogen was obtained by separating the cucumber bacterial wilt plant, and by comparing with the closely-related species 16Sr DNA sequence, it was identified as Erwinia tracheiphila and specific primers ET-P1/ET-P2 was designed. A 794 bp cucumber bacterial wilt special band can be amplified by optimizing the PCR amplification of ET-P1/ET-P2 primers with the detection sensitivity of 100 fg/μL, and can amplify the DNA of bacterial wilt cucumber and rhizosphere soil. The molecular diagnosis technology of cucumber bacterial wilt.
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References
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Proportional views
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