WANG Guang-chao, MA Lin, KANG Wen-gong, et al. The optimization research of flue-cured tobacco SSR reaction system[J]. Journal of Light Industry, 2013, 28(5): 39-42,58. doi: 10.3969/j.issn.2095-476X.2013.05.009
Citation:
WANG Guang-chao, MA Lin, KANG Wen-gong, et al. The optimization research of flue-cured tobacco SSR reaction system[J]. Journal of Light Industry, 2013, 28(5): 39-42,58.
doi:
10.3969/j.issn.2095-476X.2013.05.009
The optimization research of flue-cured tobacco SSR reaction system
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College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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Tengzhou Cigarette Factory, China Tobacco Shandong Industrial Co., Ltd., Tengzhou 277500, China
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Corresponding author:
MA Lin,
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Received Date:
2013-01-29
Available Online:
2013-09-15
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Abstract
Using flue-cured tobacco varieties RG11 as raw materials, the influence of simple sequence repeat(SSR) amplification on flue-cured tobacco SSR analysis of polymerase chain reaction(PCR) system of the main ingredients and among was studied, and the results showed that flue-cured tobacco SSR-PCR for 20 μL system, including template DNA 35 ng, 1.5 U TaqDNA polymerase, 2.375 mmol/L MgCl2, 0.6 mmol/L dNTPs, 0.4 μmol/L primer.
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References
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Proportional views
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