LI De-zhi, WANG Yun-long, LI Yu-lin, et al. GICA capture assay Mycoplasma pneumoniae in human serum IgM antibody[J]. Journal of Light Industry, 2014, 29(4): 29-32. doi: 10.3969/j.issn.2095-476X.2014.04.007
Citation:
LI De-zhi, WANG Yun-long, LI Yu-lin, et al. GICA capture assay Mycoplasma pneumoniae in human serum IgM antibody[J]. Journal of Light Industry, 2014, 29(4): 29-32.
doi:
10.3969/j.issn.2095-476X.2014.04.007
GICA capture assay Mycoplasma pneumoniae in human serum IgM antibody
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College of Life Science, He'nan Normal University, Xinxiang 453007, China;
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Henan Biotechnology Research Center, Zhengzhou 450001, China;
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College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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Center for Disease Control and Prevention of Shanyang District in Jiaozuo City, Jiaozuo 454000, China
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Received Date:
2014-05-08
Available Online:
2014-07-15
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Abstract
Using colloidal gold-labeled mouse anti-human IgM μ chain antibody,Mycoplasma pneumoniae P1 protein antigen for coating,immuno-gold preparation conditions were optimized to prepare colloidal gold immunochromatographic test strip.The test strip kit was contrasted with that in market,total coincidence rate was 99.17%.The results showed that the test strip had high sensitivity,specificity,low price.It was more suitable for MP infection in early clinical screening,and was easy and quick to operate.
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Proportional views
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