REN Su-yun, JI Hong-fei, ZHANG Zhi-hong, et al. Fabrication of sensitive layers of the 3D graphene electrochemical biosensor for heavy metal ions detection[J]. Journal of Light Industry, 2016, 31(3): 14-20. doi: 10.3969/j.issn.2096-1553.2016.3.003
Citation:
REN Su-yun, JI Hong-fei, ZHANG Zhi-hong, et al. Fabrication of sensitive layers of the 3D graphene electrochemical biosensor for heavy metal ions detection[J]. Journal of Light Industry, 2016, 31(3): 14-20.
doi:
10.3969/j.issn.2096-1553.2016.3.003
Fabrication of sensitive layers of the 3D graphene electrochemical biosensor for heavy metal ions detection
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Zhengzhou Electronic Information Engineering School, Zhengzhou 450007, China;
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School of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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State Laboratory of Surface and Interface Science of He'nan Province, Zhengzhou University of Light Industry, Zhengzhou 450001, China
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Corresponding author:
HE Ling-hao,
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Received Date:
2015-08-12
Available Online:
2016-05-15
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Abstract
The 3D graphene was prepared by redox mothod and then was functionalized by amino-modified (G-NH2). The single-stranded DNA could immobilize onto the surface of the fabricated biosensor via the electro-static interaction between DNA and G-NH2 and coordination with the relative heavy metal ions (Ag+,Hg2+,Cu2+).The surface morphology was determined using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Atom force microscopy (AFM) and X-ray photoelectrons spectroscopy (XPS) were applied to measure the chemical structure and componenets of the fabricated G-NH2 before and after being immobilized DNA.The results showed that the surface of 3D G-NH2 was smooth. Signal changes of XPS peak demonstrated that DNA were immobilize onto the surface of 3D G-NH2. The electrochemical impedance spectrum was used to characterize the electrochemical perforamance of the biosensor during the procedure of the detection of heavy metal ions. It explored that the Rct values were changed significantly after DNA was immoloilized onto the surface of 3D G-NH2 and then coordinated with the relative heavy metal ions.This study demonstrated this kind of biosensor could be used to adsorb biomolecules and detect the co-exist system of many heavy metal ions.
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