JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 31 Issue 3
May 2016
Article Contents
ZHANG Yu, ZHANG Xiao-na, XUE Qiao-li, et al. Determination of antioxidant activity of Visum ardiculatum in vitro[J]. Journal of Light Industry, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006
Citation: ZHANG Yu, ZHANG Xiao-na, XUE Qiao-li, et al. Determination of antioxidant activity of Visum ardiculatum in vitro[J]. Journal of Light Industry, 2016, 31(3): 39-48. doi: 10.3969/j.issn.2096-1553.2016.3.006 shu

Determination of antioxidant activity of Visum ardiculatum in vitro

  • Corresponding author: HU Yong-jin, 
  • Received Date: 2015-12-17
    Available Online: 2016-05-15
  • Visum ardiculatum was extracted by water bath and ultrasonic, and its four fractions of chloroform, ethyl acetate, n-butanol, and water were prepared. The free-amino acid, total polyphenol, tea polysaccharides and flavonoids contents Visum ardiculatum were determined. So were the scavenging effects of different parts of Visum ardiculatum on DPPH radical, ABTS radical, superoxide anion and hydroxyl radical and FRAP value. The results showed that the antioxidant activity of Visum ardiculatum in vitro was potent. The scavenging effects of all the fractions on ABTS radical reached 98% and that of DPPH radical reached 88%. The synthetical value calculated by fuzzy assessment was respective of the 5 antioxidant assays.The highest was 4.66 of water extracts by water bath, the lowest was 0.21 of chloroform fraction. It was largely due to the total polyphenol (P<0.05) and flavonoids contents (P<0.05).The content of tea polysaccharides reached 5 468.08 mg/100 g.
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