Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究

弓丽华; 李万鹏; 郭良起; 曲良苗; 师敬敬; 单素敏; 马伟伟; 张永辉

doi:10.3969/j.issn.2096-1553.2017.3.003

CN 41-1437/TS ISSN 2096-1553

Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究

弓丽华 , 李万鹏 , 郭良起 , 曲良苗 , 师敬敬 , 单素敏 , 马伟伟 , 张永辉

弓丽华, 李万鹏, 郭良起, 等. Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究[J]. 轻工学报, 2017, 32(3): 14-20. doi: 10.3969/j.issn.2096-1553.2017.3.003

引用本文: 弓丽华, 李万鹏, 郭良起, 等. Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究[J]. 轻工学报, 2017, 32(3): 14-20. doi: 10.3969/j.issn.2096-1553.2017.3.003

GONG Li-hua, LI Wan-peng, GUO Liang-qi, et al. Study on the efficient determination of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method[J]. Journal of Light Industry, 2017, 32(3): 14-20. doi: 10.3969/j.issn.2096-1553.2017.3.003

Citation: GONG Li-hua, LI Wan-peng, GUO Liang-qi, et al. Study on the efficient determination of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method[J]. Journal of Light Industry, 2017, 32(3): 14-20. doi: 10.3969/j.issn.2096-1553.2017.3.003

Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究

河南广电计量检测有限公司食品实验室, 河南郑州 450001;
郑州轻工业学院材料与化学工程学院, 河南郑州 450001

通讯作者: 张永辉

基金项目: 国家自然科学基金项目（21301158）；国家大学生创新创业训练项目（201510462065）
中图分类号: TS251

Study on the efficient determination of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method

Food Laboratory, He'nan GRG Metrology & Test Co., Ltd., Zhengzhou 450001, China;
College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Corresponding author: ZHANG Yong-hui,

Received Date: 2017-02-20
Available Online: 2017-05-15
CLC number: TS251

摘要: 在以乙腈-0.1%甲酸溶液为流动相、5%氨化甲醇为洗脱剂、梯度洗脱、HPLC-MS/MS电喷雾正离子、多反应监测模式的检测条件下，利用高效液相色谱串联三重四级杆质谱仪，研究了Oasis MCX固相萃取柱结合内标法检测羊肉中克伦特罗（俗称瘦肉精）残留量的方法.结果表明，克伦特罗残留量在线性范围 0.050~5.000 ng/mL 内均具有良好的检出率，相关系数为0.997 8，加标回收率为98%~102%，相对标准偏差为2.09%.该方法灵敏度高，检测结果准确度高，适用于羊肉中克伦特罗残留的定量检测，对其他肉质中克伦特罗的检测也具有一定的参考价值.
- 羊肉 /
- 瘦肉精 /
- Oasis MCX固相萃取柱 /
- 内标法
Abstract: The study aimed to establish the detection method of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method. The separation was performed by using acetonitrile-0.1% formic acid solution as mobile phase and 5% ammoniated methanol as eluent. The detection was conducted by using HPLC-ESI-MS/MS in positive ion and multiple reaction monitoring (MRM) modes. The results showed that this method possessed satisfactory detecting rate for clenbuterol ranging from 0.050 ng/mL to 5.000 ng/mL. The coefficient was 0.997 8, the recovery rate was 98%~102%, and the relative standard deviation was 2.09%. With high sensitivity and accuracy, this method could be used for detecting the clenbuterol in lamb, and it also had certain guiding significance for quantitative analysis of clenbuterol in other meat products.
- lamb /
- clenbuterol /
- Oasis MCX SPE column /
- internal standard method
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沈阳化工大学材料科学与工程学院沈阳 110142

Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究

通讯作者: 张永辉

河南广电计量检测有限公司食品实验室, 河南郑州 450001;
郑州轻工业学院材料与化学工程学院, 河南郑州 450001

收稿日期: 2017-02-20
网络出版日期: 2017-05-15

基金项目: 国家自然科学基金项目（21301158）；国家大学生创新创业训练项目（201510462065）

关键词:

摘要: 在以乙腈-0.1%甲酸溶液为流动相、5%氨化甲醇为洗脱剂、梯度洗脱、HPLC-MS/MS电喷雾正离子、多反应监测模式的检测条件下，利用高效液相色谱串联三重四级杆质谱仪，研究了Oasis MCX固相萃取柱结合内标法检测羊肉中克伦特罗（俗称瘦肉精）残留量的方法.结果表明，克伦特罗残留量在线性范围 0.050~5.000 ng/mL 内均具有良好的检出率，相关系数为0.997 8，加标回收率为98%~102%，相对标准偏差为2.09%.该方法灵敏度高，检测结果准确度高，适用于羊肉中克伦特罗残留的定量检测，对其他肉质中克伦特罗的检测也具有一定的参考价值.

English Abstract

Study on the efficient determination of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method

Corresponding author: ZHANG Yong-hui,

Food Laboratory, He'nan GRG Metrology & Test Co., Ltd., Zhengzhou 450001, China;
College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2017-02-20
Available Online: 2017-05-15

Abstract: The study aimed to establish the detection method of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method. The separation was performed by using acetonitrile-0.1% formic acid solution as mobile phase and 5% ammoniated methanol as eluent. The detection was conducted by using HPLC-ESI-MS/MS in positive ion and multiple reaction monitoring (MRM) modes. The results showed that this method possessed satisfactory detecting rate for clenbuterol ranging from 0.050 ng/mL to 5.000 ng/mL. The coefficient was 0.997 8, the recovery rate was 98%~102%, and the relative standard deviation was 2.09%. With high sensitivity and accuracy, this method could be used for detecting the clenbuterol in lamb, and it also had certain guiding significance for quantitative analysis of clenbuterol in other meat products.

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