产γ-聚谷氨酸解淀粉芽孢杆菌LDJ11培养基组分优化研究
Research on the optimization of medium components of γ-polyglutamic acid-producing Bacillus amyloliquefaciens LDJ11
-
摘要: 为了提高解淀粉芽孢杆菌LDJ11的γ-聚谷氨酸产量，在前期单因素试验的基础上，采用Plackett-Burman试验设计方案，从初始发酵培养基组分中挑选出影响γ-聚谷氨酸产量最显著的3个因素，进而采用最陡爬坡试验方法逼近γ-聚谷氨酸最高产量区域，并设计三因素三水平的Box-Behnken试验，以获得解淀粉芽孢杆菌LDJ11的最优培养基组分.结果表明：产γ-聚谷氨酸解淀粉芽孢杆菌LDJ11的最优培养基组分为：谷氨酸钠84.53 g/L，柠檬酸钠12 g/L，甘油30 g/L，（NH4）2SO4 21.67 g/L，CaCl2 0.07 g/L，MnSO4·H2O 0.1 g/L，MgSO4·7H2O 0.5 g/L，K2HPO4·3H2O 0.5 g/L，FeCl3·6H2O 0.06 g/L.在此配方下，解淀粉芽孢杆菌LDJ11的实际γ-聚谷氨酸平均产量可达37.048 g/L，是优化前产量（26.730 g/L）的1.39倍，并与理论预测最大值36.450 g/L非常接近.Abstract: In order to increase γ-polyglutamic acid production of Bacillus amyloliquefaciens LDJ11,based on the previous single-factor test, the Plackett-Burman test design was adopted.Three most significant factors that affected the yield of γ-polyglutamic acid were selected from the initial medium components. The steepest limbing test method was used to approach the maximum γ-polyglutamic acid production area,and then the optimization of the medium components was obtained through a three-fator and three-level Box-Behnken test.The results showed that the optimal medium components for γ-polyglutamic acid-producing Bacillus amyloliquefaciens LDJ11 were:sodium glutamate 84.53 g/L, sodium citrate 12 g/L, glycerol 30 g/L,(NH4)2SO4 21.67 g/L, CaCl2 0.07 g/L, MnSO4·H2O 0.1 g/L,MgSO4·7H2O 0.5 g/L,K2HPO4·3H2O 0.5 g/L,FeCl3·6H2O 0.06 g/L. Under this condition, the actual yield of γ-polyglutamic acid produced by Bacillus amyloliquefaciens LDJ11 was 37.048 g/L, which was 1.39 times of the initial medium component yield (26.730 g/L),and it was very close to the theoretical prediction of 36.450 g/L.
-
-
[1]
刘晓鸥,李睿颖,徐勇虎,等.聚谷氨酸的生物合成及应用前景[J].食品工程,2009(1):23.
-
[2]
IYANOVICS G,BRUCKNER V.Chemische und immunologische studien uber den mechanismus der milzbrandinfektion und immunitat die chemische struktur der kapelsubstanz des milzbrandbacillus und der serolisch identischen spezifichen substanz des Bacillus mesentericus[J].Zeitschrift für Immunitts for Schung,1937(90):304.
-
[3]
YOON S H,DO J H,LEESY,et al.Production of poly-γ-glutamic acid by fed-batch culture of Bacillus licheniformis[J].Biotechnology Letters,2000(22):585.
-
[4]
曹名锋,金映虹,解慧,等.γ-PGA的微生物合成、相关基因及应用展望[J].微生物学通报,2011(3):388.
-
[5]
张海群,张智维,刘婷.γ-聚谷氨酸产生菌发酵培养基的响应面法优化研究[J].粮油食品科技,2015(1):79.
-
[6]
程艳玲,赵玉娥,王海玉.生物降解型聚谷氨酸的研究进展[J].北京联合大学学报(自然科学版),2008(2):45.
-
[7]
王普,孙立明,何军邀.响应面法优化热带假丝酵母104菌株产羰基还原酶发酵培养基[J].生物工程学报,2009,25(6):863.
-
[8]
侯学敏,李林霞,张直峰,等.响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性[J].食品科学,2013(6):124.
-
[9]
BOX G E P,WILSON K B.On the experimental attainment of optimum conditions[J].Journal of the Royal Statistical Society,1951(1):1.
-
[10]
王永菲,王成国.响应面法的理论与应用[J]. 中央民族大学学报(自然科学版),2005,14(3):236.
-
[11]
BOX G E P.Statistics for experiments:an introduction to design,data analysis and model building[M].New York:Wiley,1990.
-
[12]
张泽志,韩春亮,李成未.响应面法在试验设计与优化中的应用[J].河南教育学院学报(自然科学版),2011(4):34.
-
[13]
范洪臣,李艳华,梁金钟,等.响应面法优化枯草芽孢杆菌产γ-PGA的条件[J].生物加工过程,2008,6(3):17.
-
[14]
王允样,吕凤霞,陆兆新.杯伞发酵培养基的响应曲面法优化研究[J].南京农业大学学报,2004(3):89.
-
[1]
-
点击查看大图
计量
- PDF下载量: 25
- 文章访问数: 1137
- 引证文献数: 0
下载:
