高产右旋糖苷酶微生物的筛选及产酶条件的优化

黄丽; 孟继坤; 周嫄; 李树波; 徐羽

doi:10.3969/j.issn.2096-1553.2019.01.002

January 2019

Article Contents

HUANG Li, MENG Jikun, ZHOU Yuan, et al. Screening of high yield dextran microorganisms and optimization of enzyme production conditions[J]. Journal of Light Industry, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002

Citation: HUANG Li, MENG Jikun, ZHOU Yuan, et al. Screening of high yield dextran microorganisms and optimization of enzyme production conditions[J]. Journal of Light Industry, 2019, 34(1): 11-20. doi: 10.3969/j.issn.2096-1553.2019.01.002 shu

Screening of high yield dextran microorganisms and optimization of enzyme production conditions

School of Light Industry and Food Engineering, Guangxi University, Nanning 530000, China

Received Date: 2018-10-16

Abstract

The microorganism SGC-2 with high yield of dextran glycosidase was screened from sugarcane soil using nutrient-deficient medium and transparent circle method. The microorganism SGC-2 was identified according to its morphology and 16S rDNA gene sequence analysis. Moreover, the conditions of fermentation and enzyme production were further optimized. The results showed that the microorganism SGC-2 was Bacillus megatherium. The optimum conditions for enzyme production were as follows: dextran T2000 20 g/L, beef extract 10 g/L, NaCl 4 g/L, MgSO₄ 0.4 g/L, inoculation time 36 h, inoculation volume 3%, fermentation time 96 h. Under this condition, the activity of dextran anhydrase reached 28.32 U/mL, which increased by 151% when compared with that before optimization. The dextran anhydrase-producing strain was firstly reported, which provided a theoretical basis for screening high-yield dextran microorganisms.
- Polymer dextran,
- dextran,
- Bacillus megatherium,
- fermentation optimization
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沈阳化工大学材料科学与工程学院沈阳 110142

Screening of high yield dextran microorganisms and optimization of enzyme production conditions

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