JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

高通量测序分析合生元制剂对人体肠道菌群组成的影响

鲍志宁 ,  甘祥武 ,  邓均尹 ,  吕思行

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鲍志宁, 甘祥武, 邓均尹, 等. 高通量测序分析合生元制剂对人体肠道菌群组成的影响[J]. 轻工学报, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
引用本文: 鲍志宁, 甘祥武, 邓均尹, 等. 高通量测序分析合生元制剂对人体肠道菌群组成的影响[J]. 轻工学报, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
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BAO Zhining, GAN Xiangwu, DENG Junyin and et al. Effect of synbiotic preparation on gut microbiota in human evaluated by high-throughput sequencing[J]. Journal of Light Industry, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
Citation: BAO Zhining, GAN Xiangwu, DENG Junyin and et al. Effect of synbiotic preparation on gut microbiota in human evaluated by high-throughput sequencing[J]. Journal of Light Industry, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
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高通量测序分析合生元制剂对人体肠道菌群组成的影响

  • 广州市微生物研究所, 广东 广州 510663

    • 作者简介: 鲍志宁(1983-),女,山东省烟台市人,广州市微生物研究所高级工程师,博士,主要研究方向为食品生物技术.;

  • 基金项目: 广州市珠江新星专项项目（201806010070）

  • 中图分类号: Q935

Effect of synbiotic preparation on gut microbiota in human evaluated by high-throughput sequencing

  • Guangzhou Institute of Microbiology, Guangzhou 510663, China

  • Received Date: 2019-07-23

    CLC number: Q935

  • 摘要: 通过健康成年人连续服用HZB-1合生元制剂对其肠道菌群进行干预，利用高通量测序技术对干预前后受试人员肠道菌群结构进行分析，探究服用HZB-1合生元制剂对人体肠道菌群组成的影响.结果表明：采用HZB-1合生元制剂干预后，受试人员肠道菌群的丰富度、多样性均有明显改善；在"门"水平上，受试人员肠道菌群丰度明显增加，其中厚壁菌门（Firmicutes）占比增高，放线菌门（Actinobacteria）和变形菌门（Proteobacteria）存在一定的动态平衡；在"属"水平上，布劳特氏菌属（Blautia）、柔嫩梭菌属（Faecalibacterium）、吉米菌属（Gemmiger）等有益菌群丰度明显增加，而嗜血杆菌属（Haemophilus）、Parasutterella菌属等有害菌属丰度明显降低.这表明，合生元制剂的摄入，对肠道菌群结构起到有益的调节作用.
    Abstract: To explore the changes of intestinal microbiota in healthy human by oral HZB-1synbiotic, the intestinal flora belong intervention was amplified by high-throughput sequencing,ensuring the effect of synbiotic preparation. The experiment showed that the abundance and diversity of the intestinal microbiota of the subjects were significantly improved. At the "gate" level, the abundance of intestinal microbiota increased significantly and the proportion of Firmicutes increased. There was a certain dynamic balance between Actinobacteria and Proteobacteria. At the "genus" level, the abundance of beneficial bacteria such as Blautia, Faecalibacterium and Gemmiger increased significantly, while the abundance of harmful bacteria such as Haemophilus and Parasutterella reduced significantly.This indicated that the intake of synbiotic preparation played a beneficial role in regulating the structure of gut microbiota.
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鲍志宁, 甘祥武, 邓均尹, 等. 高通量测序分析合生元制剂对人体肠道菌群组成的影响[J]. 轻工学报, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
引用本文: 鲍志宁, 甘祥武, 邓均尹, 等. 高通量测序分析合生元制剂对人体肠道菌群组成的影响[J]. 轻工学报, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
shu
BAO Zhining, GAN Xiangwu, DENG Junyin and et al. Effect of synbiotic preparation on gut microbiota in human evaluated by high-throughput sequencing[J]. Journal of Light Industry, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
Citation: BAO Zhining, GAN Xiangwu, DENG Junyin and et al. Effect of synbiotic preparation on gut microbiota in human evaluated by high-throughput sequencing[J]. Journal of Light Industry, 2019, 34(6): 1-8. doi: 10.3969/j.issn.2096-1553.2019.06.001
shu

高通量测序分析合生元制剂对人体肠道菌群组成的影响

    作者简介:鲍志宁(1983-),女,山东省烟台市人,广州市微生物研究所高级工程师,博士,主要研究方向为食品生物技术.
  • 广州市微生物研究所, 广东 广州 510663
  • 收稿日期: 2019-07-23
基金项目:  广州市珠江新星专项项目（201806010070）

摘要: 通过健康成年人连续服用HZB-1合生元制剂对其肠道菌群进行干预，利用高通量测序技术对干预前后受试人员肠道菌群结构进行分析，探究服用HZB-1合生元制剂对人体肠道菌群组成的影响.结果表明：采用HZB-1合生元制剂干预后，受试人员肠道菌群的丰富度、多样性均有明显改善；在"门"水平上，受试人员肠道菌群丰度明显增加，其中厚壁菌门（Firmicutes）占比增高，放线菌门（Actinobacteria）和变形菌门（Proteobacteria）存在一定的动态平衡；在"属"水平上，布劳特氏菌属（Blautia）、柔嫩梭菌属（Faecalibacterium）、吉米菌属（Gemmiger）等有益菌群丰度明显增加，而嗜血杆菌属（Haemophilus）、Parasutterella菌属等有害菌属丰度明显降低.这表明，合生元制剂的摄入，对肠道菌群结构起到有益的调节作用.

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