阳荷多糖提取工艺优化及其生物活性研究
Optimization of extraction process and evaluation of biological activities of polysaccharides from Zingiber striolatum
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摘要: 为拓展特色植物资源阳荷(Zingiber striolatum)的利用途径,提高其产品附加值,以新鲜阳荷为原料,采用水提醇沉法提取阳荷多糖(Zingiber striolatum polysaccharide,ZSP),利用单因素试验和响应面试验优化ZSP的提取工艺,并对ZSP的乳化性、抑菌性、重金属吸附能力、抗氧化活性及降血糖活性进行研究。结果表明:ZSP的最优提取工艺为粉碎目数80目、料液比1∶65、提取温度75 ℃、提取时间96 min、乙醇添加量400 mL,在此条件下,ZSP得率为(3.89±0.01)%;ZSP的乳化性随其质量浓度的增加而增大,当其质量浓度为15 mg/mL时,乳化性最高,为(76.14±1.33)%;ZSP对大肠杆菌有抑制作用,且抑菌性随其质量浓度的增加而增强,当其质量浓度为50 mg/mL时,抑菌圈直径为(2.35±0.02) cm;ZSP对Cu2+、Cd2+和Pb2+的吸附量均随其质量浓度的增加而增大,当其质量浓度为1.0 g/L时,吸附量分别达(17.26±0.41) mg/g、(21.19±0.33) mg/g和(27.64±0.61) mg/g;ZSP对ABTS+、DPPH和·OH自由基的清除率均随其质量浓度的增加而增大,当其质量浓度为3 mg/mL时,清除率分别达(52.10±0.01)%、(60.40±1.95)%和(22.20±0.02)%;ZSP对α-葡萄糖苷酶的抑制率也随其质量浓度的增加而增大,当其质量浓度为1.0 mg/mL时,抑制率达到最大值(38.00±2.00)%。综上可知,ZSP在重金属吸附和降血糖方面表现较突出,表明其在开发促排重金属的可食性材料及抗糖尿病功能产品方面具有较好的应用前景。Abstract: To expand the utilization of Zingiber striolatum (a characteristic plant resource) and enhance the value-added potential of its products, Zingiber striolatum polysaccharides (ZSP) were extracted from fresh Zingiber striolatum using aqueous extraction followed by ethanol precipitation. The extraction process was optimized via single-factor and response surface experiments, and the biological activities of ZSP were evaluated, including emulsifying property, antibacterial activity, heavy metal adsorption capacity, antioxidant activity, and hypoglycemic potential. The optimal extraction parameters were: particle size 80 mesh, solid-liquid ratio 1∶65, extraction temperature 75 ℃, extraction time 96 min, and ethanol volume 400 mL. Under these conditions, the ZSP yield was (3.89±0.01)%. The emulsifying property of ZSP increased with higher mass concentration, reaching a maximum of (76.14±1.33)% at 15 mg/mL. ZSP exhibited antibacterial activity against Escherichia coli, with inhibition zone diameter increasing at higher concentrations; at 50 mg/mL, the inhibition zone diameter was (2.35±0.02) cm. The adsorption capacities of ZSP for Cu2+, Cd2+ and Pb2+ increased at higher mass concentrations, reaching (17.26±0.41) mg/g, (21.19±0.33) mg/g, and (27.64±0.61) mg/g at 1.0 g/L, respectively. The scavenging activities of ZSP against ABTS+, DPPH, and ·OH radicals increased with higher mass concentration, with scavenging rates of (52.10±0.01)%, (60.40±1.95)%, and (22.20±0.02)% at 3 mg/mL, respectively. The inhibitory activity of ZSP against α-glucosidase increased with higher mass concentration, reaching a maximum of (38.00±2.00)% at 1.0 mg/mL. Collectively, ZSP demonstrated significant potential in heavy metal adsorption and hypoglycemic activity, suggesting its promise for developing edible materials for heavy metal removal and antidiabetic functional products.
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Key words:
- Zingiber striolatum /
- polysaccharide /
- extraction process /
- biological activity
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