基于GC-MS结合多元统计的2种工艺提取脐橙挥发性成分差异分析

杨鹏飞; 吴薇; 孙海峰; 刘龑章; 尚紫博; 薛晶晶; 许志杰; 宋梦坤

doi:10.12187/2025.04.001

基于GC-MS结合多元统计的2种工艺提取脐橙挥发性成分差异分析

杨鹏飞 ¹ ,
吴薇 ¹ ,
孙海峰 ² ,
刘龑章 ¹ ,
尚紫博 ¹ ,
薛晶晶 ¹ ,
许志杰 ³ ,
宋梦坤 ^{4

,

,}

1. 郑州轻工业大学烟草科学与工程学院, 河南郑州 450001;
2. 深圳烟草工业有限责任公司技术研发中心, 广东深圳 518000;
3. 贝尔香精香料(上海)有限公司, 上海 201615;
4. 湖北中烟工业有限责任公司技术中心, 湖北武汉 430040

作者简介: 杨鹏飞(1987—),男,河南省洛阳市人,郑州轻工业大学副教授,博士,主要研究方向为香精香料和天然产物化学。E-mail:pf_yang@zzuli.edu.cn;

通讯作者: 宋梦坤,smkun@xf.hbtobacco.cn

基金项目: 中国烟草总公司重大科技项目(110202201053(SJ-03))
国家自然科学基金青年基金资助项目(81903507)
中图分类号: TS202.3

Analysis of the differences in volatile components of navel oranges extracted by two processes based on GC-MS combined with multivariate statistics

1. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. Technology R&D Center, Shenzhen Tobacco Industrial Co., Ltd., Shenzhen 518000, China;
3. Bell Flavors & Fragrances (Shanghai) Co., Ltd., Shanghai 201615, China;
4. Technology Center, China Tobacco Hubei Industrial Co., Ltd., Wuhan 430040, China

Corresponding author: SONG Mengkun, smkun@xf.hbtobacco.cn

Received Date: 2024-07-03
Accepted Date: 2024-08-24

CLC number: TS202.3

摘要: 通过旋转锥体柱(SCC)和水蒸气蒸馏(SD)工艺提取脐橙挥发性成分,采用气相色谱-质谱联用(GC-MS)技术测定挥发性成分种类和含量,通过感官评价,结合多元统计分析方法识别判定2种工艺获得的主要差异挥发性成分。结果表明,SCC提取的挥发性成分提取率显著高于SD,SCC提取的挥发性成分具有较强的果香、甜香、花香和青香,而SD提取的挥发性成分则表现出更强的脂香和木香。2种工艺提取的挥发性成分种类和含量存在明显差异,共提取出110种挥发性成分,其中,SCC工艺97种,SD工艺68种;以P<0.05且变量重要性投影(VIP)>1为依据筛选出33种差异挥发性成分,其中月桂烯、棕榈油酸、(E)-β-罗勒烯、棕榈酸乙酯等与木香和脂香相关性较强,(Z)-橙花叔醇、α-松油醇、D-柠檬烯、巴伦西亚橘烯等则与果香、花香、甜香相关性较强,说明SCC可高效提取该类成分从而保留脐橙新鲜自然的香味。
- 脐橙 /
- 挥发性成分 /
- 旋转锥体柱 /
- 水蒸气蒸馏 /
- 多元统计分析
Abstract: Volatile components of navel oranges were extracted using spinning cone column (SCC) and steam distillation (SD) methods. Qualitative and quantitative analyses were conducted via gas chromatography-mass spectrometry (GC-MS). Sensory evaluation combined with multivariate statistical analysis was employed to identify and characterize the key differential volatile components from the two processes.Results showed that the extraction rate of volatile components using SCC was significantly higher than that using SD. Volatile components extracted via SCC exhibited stronger fruity, sweet, floral, and green notes, while those from SD showed stronger fatty and woody notes. Notable differences were observed in the types and contents of volatile components extracted by the two methods, with a total of 110 components identified (97 via SCC and 68 via SD).Based on P<0.05 and variable importance in projection (VIP)>1 criteria, 33 differential volatile components were identified. Among these, myrcene, palmitoleic acid, (E)-β-ocimene, and ethyl palmitate were positively correlated with woody and fatty notes, whereas (Z)-nerolidol, α-terpineol, D-limonene, and valencene were positively correlated with fruity, floral, and sweet notes. This indicated that the SCC could efficiently extract these components, thereby preserving the fresh and natural aroma of navel oranges.
- navel orange /
- volatile component /
- spinning cone column /
- steam distillation /
- multivariate statistical analysis
1. [1]
  李晋,张琛,刘红,等.近红外光谱联合化学计量学在柑橘类水果质量无损检测方面的最新研究及应用进展[J].食品与发酵工业,2024,50(5):367-379.
2. [2]
  车金鑫,郭虹雨,陈珂琴,等.柑橘废弃物利用的研究进展[J].食品与发酵工业,2022,48(20):300-306.
3. [3]
  崔佳韵,梁建芬.柑橘属植物果皮精油抑菌作用研究进展[J].粮油食品科技,2018,26(1):35-39.
4. [4]
  SHAW D,TRIPATHI A D,PAUL V,et al.Valorization of essential oils from citrus peel powder using hydro-distillation[J].Sustainable Chemistry and Pharmacy,2023,32: 101036.
5. [5]
  BARANSE O,JESUS E,MARIA M G.Deterpenation of citrus essential oils using glycerol-based deep eutectic solvents[J].Journal of Chemical & Engineering Data,2018,63(7): 2384-2393.
6. [6]
  RUSSO M,RIGANO F,ARIGO A,et al.Coumarins,psoralens and polymethoxyflavones in cold-pressed citrus essential oils: A review[J].Journal of Essential Oil Research,2021,33(3): 221-239.
7. [7]
  SONG Z L,WEI X X,XIE M M,et al.Study on the microwave extraction process and product distribution of essential oils from citrus peel[J].Chemical Engineering and Processing-Process Intensification,2022,171: 108726.
8. [8]
  MANDE P,SEKAR N.Comparison of chemical composition,antioxidant and antibacterial activity of Callistemon citrinus skeels (bottlebrush) essential oil obtained by conventional and microwave-assisted hydrodistillation[J].Journal of Microwave Power and Electromagnetic Energy,2020,54(3): 230-244.
9. [9]
  白雨佳,张丽荣,黄佳琪,等.压榨法制备甜橙油的研究[J].食品工业,2015,36(7):87-90.
10. [10]
  孙正光,杨晓东,吴薇,等.基于气味活性值的旋转锥柱处理前后黄瓜特征香味成分分析[J].食品与发酵工业,2024,50(18):309-316.
11. [11]
  PAREDES D A F,SANCHEZ R J,MORERO B,et al.Enriching the conceptual modelling approach with environmental considerations: Application to the partial dealcoholization of wines[J].Separation and Purification Technology,2023,308: 122950.
12. [12]
  LUO Y,WANG K,ZHUANG H N,et al.Elucidation of aroma compounds in passion fruit(Passiflora alata Ait) using a molecular sensory approach[J].Journal of Food Biochemistry,2022,46(9): e14224.
13. [13]
  李欣,华建新,罗杰洪,等.不同提取方法对井冈蜜柚皮精油组成与性质的影响[J].食品工业科技,2024,45(3):83-97.
14. [14]
  SHUI M Z,FENG T,TONG Y Z,et al.Characterization of key aroma compounds and construction of flavor base module of Chinese sweet oranges[J].Molecules,2019,24(13): 2384-2384.
15. [15]
  刘菲斐,陈晋,陈健乐,等.基于化学计量学鉴定不同甜橙精油挥发性成分[J].食品科学,2024,45(7):155-163.
16. [16]
  高阳,赵生,许式强,等.龙井茶挥发油的旋转锥体柱提取及其应用研究[J].食品工业,2015,36(5):120-124.
17. [17]
  李丽,蒋景龙,胡佳乐,等.不同品种柑橘果皮精油关键香气物质筛选[J].食品工业科技,2023,44(24):259-269.
18. [18]
  马冬,吕树萍,米兰芳,等.赣脐4号脐橙果实挥发性组分分析[J].赣南师范大学学报,2023,44(3):76-82.
19. [19]
  CHENG Y J,HAN L,HUANG L H,et al.Association between flavor composition and sensory profile in thermally processed mandarin juices by multidimensional gas chromatography and multivariate statistical analysis.[J].Food Chemistry,2023,419: 136026.
20. [20]
  余汉谋,姜兴涛,肖海鸿.旋转锥体柱技术及其在食品和香精香料行业中的应用进展[J].食品工业科技,2013,34(24):372-375.
21. [21]
  NGUYEN H,CAMPI M E,JACKSON R W,et al.Effect of oxidative deterioration on flavour and aroma components of lemon oil[J].Food Chemistry,2008,112(2): 388-393.
22. [22]
  赵甜甜,张国治,王赵改,等.两种市售香椿茶主要活性成分、抗氧化活性及挥发性成分的对比分析[J].轻工学报,2023,38(3):35-45.
23. [23]
  帖金鑫,张青松,李永生,等.加拿大烟叶与云南KRK26烟叶香气成分差异分析[J].轻工学报,2023,38(5):74-82.
24. [24]
  HUANG X W,ZHAO L H,PANG S,et al.Effects of varieties,cultivation methods,and origins of citrus sinensis 'hongjiang’ on volatile organic compounds: HS-SPME-GC/MS analysis coupled with OPLS-DA[J].Agriculture,2022,12(10): 1725-1726.
25. [25]
  SHAO S X,XU M T,LIN Y P,et al.Analysis of aroma differences of Huang Guanyin oolong tea from different origins based on electronic nose and HS-SPME-GC-MS techniques[J].Food Science,2023,44(4): 232-239.
26. [26]
  吕先绪,程志魁,王东登,等.香芋冬瓜果实挥发性香气化合物的固相微萃取优化及解析[J].南方农业学报,2021,52(11):3067-3075.
27. [27]
  刘煜宇,包秀萍,罗海涛,等.陈香普洱茶净油分子蒸馏工艺的响应面法优化[J].食品工业,2021,42(9): 102-105.
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沈阳化工大学材料科学与工程学院沈阳 110142

基于GC-MS结合多元统计的2种工艺提取脐橙挥发性成分差异分析

作者简介: 杨鹏飞(1987—),男,河南省洛阳市人,郑州轻工业大学副教授,博士,主要研究方向为香精香料和天然产物化学。E-mail:pf_yang@zzuli.edu.cn;

通讯作者: 宋梦坤,smkun@xf.hbtobacco.cn

Analysis of the differences in volatile components of navel oranges extracted by two processes based on GC-MS combined with multivariate statistics

Corresponding author: SONG Mengkun, smkun@xf.hbtobacco.cn

计量

通讯作者: 陈斌, bchen63@163.com

基于GC-MS结合多元统计的2种工艺提取脐橙挥发性成分差异分析

作者简介:杨鹏飞(1987—),男,河南省洛阳市人,郑州轻工业大学副教授,博士,主要研究方向为香精香料和天然产物化学。E-mail:pf_yang@zzuli.edu.cn

通讯作者: 宋梦坤, smkun@xf.hbtobacco.cn

English Abstract

Analysis of the differences in volatile components of navel oranges extracted by two processes based on GC-MS combined with multivariate statistics

Corresponding author: SONG Mengkun, smkun@xf.hbtobacco.cn

目录

基于GC-MS结合多元统计的2种工艺提取脐橙挥发性成分差异分析

作者简介: 杨鹏飞(1987—),男,河南省洛阳市人,郑州轻工业大学副教授,博士,主要研究方向为香精香料和天然产物化学。E-mail:pf_yang@zzuli.edu.cn;

通讯作者: 宋梦坤,smkun@xf.hbtobacco.cn

Analysis of the differences in volatile components of navel oranges extracted by two processes based on GC-MS combined with multivariate statistics

Corresponding author: SONG Mengkun, smkun@xf.hbtobacco.cn

计量

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通讯作者: 陈斌, bchen63@163.com

基于GC-MS结合多元统计的2种工艺提取脐橙挥发性成分差异分析

作者简介:杨鹏飞(1987—),男,河南省洛阳市人,郑州轻工业大学副教授,博士,主要研究方向为香精香料和天然产物化学。E-mail:pf_yang@zzuli.edu.cn

通讯作者: 宋梦坤, smkun@xf.hbtobacco.cn

English Abstract

Analysis of the differences in volatile components of navel oranges extracted by two processes based on GC-MS combined with multivariate statistics

Corresponding author: SONG Mengkun, smkun@xf.hbtobacco.cn

目录