五氯苯酚对污泥急性毒性和菌群结构的影响
Effects of pentachlorophenol on sludge acute toxicity and microbial community
-
摘要: 利用易于控制的序批式反应器（SBR）处理以甲醇为共代谢碳源的五氯苯酚（PCP）废水，控制进水PCP质量浓度为10 mg/L，并设置仅以甲醇为碳源的对照组，利用发光菌急性毒性生物实验和变性梯度凝胶电泳技术分别研究PCP对污泥急性毒性和菌群结构的影响，进而分析优势菌群对污泥急性毒性的作用.结果表明，在第1-19 d期间，进水中的PCP（10 mg/L）会严重抑制污泥活性，导致污泥絮体解体，PCP难以被降解去除，出水化学需氧量（COD）质量浓度和污泥急性毒性显著高于对照组；受PCP污染的污泥活性恢复较慢，在第20-32 d期间不再投加PCP和在第33-75 d期间补充投加2 mg/L PCP，进水COD和PCP的处理效果仍旧较差；吸附至污泥的PCP仅仅是引起污泥急性毒性升高的原因之一，受PCP诱导富集的优势菌群在降解PCP过程中产生的代谢中间产物和分泌的次级代谢产物也是引起污泥急性毒性不可忽视的重要因素.Abstract: A sequencing batch bioreactor (SBR) was used to treat pentachlorophenol (PCP) wastewater with the co-metabolic carbon source of methanol,and the influent PCP was controlled as 10 mg/L.In addition,another SBR only supplied methanol was set as control.The effects of PCP on sludge acute toxicity and microbial community were studied by the luminescent bacterium acute toxicity bioassy and denaturing gradient gel electrophoresis technique,and the function of dominant microbes on sludge acute toxicity was deeply analyzed.Results showed that during the period of 1-19 days,the sludge activity was significantly inhibited after injecting into 10 mg/L PCP in influent,the sludge flocs were disintegrated and the PCP was difficult to degrade.The effluent chemical oxygen demands (COD) and sludge acute toxicity under PCP stress were significantly higher than the control.The regeneration of sludge activity was slow due to the PCP contamination.No PCP was added during 20-32 days and 2 mg/L PCP was added during 33-75 days,the removal of influent COD and PCP was still poor.The adsorbed PCP in sludge causing the increase of sludge acute toxicity was only one of the reasons.The metabolic intermediates of PCP and secretive secondary metabolites by the enriched dominant microbes should not be ignored in the process of PCP degradation,which played an important role in causing the sludge acute toxicity.
-
-
[1]
MONSALVO V M,MOHEDANO A F,CASAS J A,et al.Cometabolic biodegradation of 4-chlorophenol by sequencing batch reactors at different temperatures[J].Bioresource Technology,2009,100(20):4572.
-
[2]
LIM J W,LIM P E,SENG C E,et al.Simultaneous 4-chlorophenol and nitrogen removal in moving bed sequencing batch reactors packed with polyurethane foam cubes of various sizes[J].Bioresource Technology,2013,129(2):485.
-
[3]
LIU Y J,LIU J,ZHANG A N,et al.Treatment effects and genotoxicity relevance of the toxic organic pollutants in semi-coking wastewater by combined treatment process[J].Environmental Pollution,2017,220:13.
-
[4]
TOCZYŁOWSKA-MAMIŃSKA R.Limits and perspectives of pulp and paper industry wastewater treatment:A review[J].Renewable and Sustainable Energy Reviews,2017,78:764.
-
[5]
姜梅,牛世全,展惠英,等.氯酚类化合物的微生物降解研究进展[J].应用生态学报,2003,14(6):1003.
-
[6]
WEN J P,LI H M,BAI J,et al.Biodegradation of 4-chlorophenol by Candida albicans PDY-07 under anaerobic conditions[J].Chinese Journal of Chemical Engineering,2006,14(6):790.
-
[7]
夏青,陈艳卿,刘宪兵.水质基准与水质标准[M].北京:中国标准出版社,2004.
-
[8]
朱冬梅.表面改性活性炭对水中三氯酚的去除及再生实验研究[D].南昌:南昌大学,2009.
-
[9]
BIAN W J,SONG X H,LIU D Q,et al.The intermediate products in the degradation of 4-chlorophenol by pulsed high voltage discharge in water[J].Journal of Hazardous Materious,2011,192(3):1330.
-
[10]
CHEN A W,ZENG G M,CHEN G Q,et al.Hydrogen sulfide alleviates 2,4-dichlorophenol toxicity and promotes its degradation in Phanerochaete chrysosporium[J].Chemosphere,2014,109:208.
-
[11]
KARN S K,CHAKRABARTY S K,REDDY M S.Pentachlorophenol degradation by Pseudomonas stutzeri CL7 in the secondary sludge of pulp and paper mill[J].Journal of Environmental Science,2010,22(10):1608.
-
[12]
ROIG N,SIERRA J,NADAL M,et al.Relationship between pollutant content and ecotoxicity of sewage sludges from Spanish wastewater treatment plants[J].Science of the Total Environment,2012,425:99.
-
[13]
ZHAO J G,CHEN X R,LIN F K,et al.Mechanism of toxicity formation and spatial distribution in activated sludge treating synthetic effluent containing bisphenol A (BPA)[J].Chemical Engineering Journal,2014,250:91.
-
[14]
国家环境保护总局.水和废水监测方法[M].第4版.
北京:中国环境科学出版社. -
[15]
KHODADOUST A P,WAGNER J A,SUIDAN M T,et al.Anaerobic treatment of PCP in fluidized-bed GAC bioreactors[J].Water Research,1997,31(7):1776.
-
[16]
BOTT C B,LOVE N G.The immunochemical detection of stress proteins in activated sludge exposed to toxic chemicals[J].Water Research,2001,35(1):91.
-
[17]
RUCKDESCHEL G,RENNER G,SCHWARZ K.Effects of pentachlorophenol and some of its known and possible metabolites on different species of bacteria[J].Applied and Environmental Microbiology,1987,53(11):2689.
-
[18]
ROSS N,DESCHENES L,BUREAU J.Ecotoxicological assessment and effects of physicochemical factors on biofilm development in groundwater conditions[J].Environmental Science and Technology,1998,32(8):1105.
-
[19]
SEKIGUCHI H,TOMIOKA N,NAKAHARA T,et al.A single band does not always represent single bacterial strains in denaturing gradient gel electrophoresis analysis[J].Biotechnology Letters,2001,23(15):1205.
-
[20]
ZHAO J G,CHEN X R,BAO L L,et al.Correlation between microbial diversity and toxicity of sludge treating synthetic wastewater containing 4-chlorophenol in sequencing batch reactors[J].Chemosphere,2016,153:138.
-
[21]
BOONNORAT J,CHIEMCHAISRI C,CHIEMCHAISRI W,et al.Microbial adaptation to biodegrade toxic organic micro-pollutants in membrane bioreactor using different sludge sources[J].Bioresource Technology,2014,165:50.
-
[22]
NAYAK A S,VIJAYKUMAR M H,KAREGOUDAR T B.Characterization of biosurfactant produced by Pseudoxanthomonas sp.PNK-04 and its application in bioremediation[J].International Biodeterioration & Biodegradation,2009,63(1):73.
-
[23]
DENTON M,KERR K G.Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia[J].Clinical Microbiology Reviews,1998,11(1):57.
-
[24]
PINGAKONG O,HABE H,OMORI T.The unique aromatic catabolic genes in sphingomonads degrading polycyclic aromatic hydrocarbons (PAHs)[J].Journal of General and Applied Microbiology,2003,49(1):1.
-
[25]
VUILLEUMIER S.Bacteial glutathione S-transferases:What are they good for?[J].Journal of Bacteriology,1997,179(5):1431.
-
[26]
GOMILA M,TVRZOVÁ L,TESHIM A,et al.Achromobacter marplatensis sp.nov.,isolated from a pentachlorophenol-contaminated soil[J].International Journal of Systematic and Evolutionary Microbiology,2011,61:2231.
-
[1]
-
点击查看大图
计量
- PDF下载量: 12
- 文章访问数: 3537
- 引证文献数: 0
下载:
