基于高通量测序和纯培养联用技术的药曲微生物多样性解析
Analysis of microbial diversity in meidicinal koji based on high-throughput sequencing and pure culture techniques
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摘要: 采用MiSeq高通量测序和纯培养联用技术对采集自江西省南昌市的药曲样品进行微生物多样性解析,以揭示药曲中的微生物群落结构。结果表明:药曲中细菌类群的丰富度和多样性均明显高于真菌类群,细菌主要优势属为片球菌属(Pediococcus)和促生乳杆菌属(Levilactobacillus),平均相对含量分别为38.04%和13.00%,真菌优势属为假丝酵母属(Candida)和复膜孢酵母属(Saccharomycopsis),平均相对含量分别为78.30%和17.92%。从药曲中分离出的9株细菌被鉴定为戊糖片球菌(Pediococcus pentosaceus)、乳酸片球菌(Pediococcus acidilactici)和地衣芽孢杆菌(Bacillus licheniformis)3个种,7株真菌被鉴定为扣囊复膜酵母(Saccharomycopsis fibuligera)、马兰加酵母(Saccharomycopsis malanga)、库德里阿兹威毕赤酵母(Pichia kudriavzevii)和酿酒酵母(Saccharomyces cerevisiae)4个种。
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关键词:
- 药曲 /
- MiSeq高通量测序 /
- 纯培养 /
- 微生物多样性
Abstract: MiSeq high-throughput sequencing and pure culture techniques were combined to analyze the microbial diversity of medicinal koji samples collected from Nanchang city, Jiangxi province, aiming to reveal the microbial composition within the koji. The results showed that the richness and diversity of bacterial taxa in the medicinal koji included in this study were significantly higher than those of the fungus. The dominant bacterial genera were Pediococcus and Levilactobacillus, with an average relative content of 38. 04% and 13. 00%, respectively. The dominant fungus genera were Candida and Saccharomycopsis, with an average relative content of 78. 30% and 17. 92%, respectively. Nine strains of bacteria isolated from medicinal koji were identified as three species: Pediococcus pentosaceus, Pediococcus acidilactici, and Bacillus licheniformis. Seven strains of fungus were identified as four species: Saccharomycopsis fibuligera, Saccharomycopsis malanga, Pichia kudriavzevii, and Saccharomyces cerevisiae.-
Key words:
- medicinal koji /
- MiSeq high-throughput sequencing /
- pure culture /
- microbial diversity
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[1]
HOR P K,GOSWAMI D,GHOSH K,et al. Preparation of rice fermented food using root of Asparagus racemosus as herbal starter and assessment of its nutrient profile[J]. Systems Microbiology and Biomanufacturing,2022,2(1):147-156.
-
[2]
吴志,陈晓芸.甜酒曲主要微生物的分离、筛选及其中草药制曲工艺研究[J].轻工科技,2020,36(5):18-20.
-
[3]
江威,李强,唐洁,等.中药材对清香型白酒主要酿造微生物及风味物质的影响[J].中国酿造, 2023, 42(2):89-94.
-
[4]
梁敏华,赵文红,白卫东,等.白酒酒曲微生物菌群对其风味形成影响研究进展[J].中国酿造,2023,42(5):22-27.
-
[5]
QUIJADA N M, HERNÁNDEZ M, RODRÍGUEZLAZÁRO D. High-throughput sequencing and food microbiology[J]. Advances in Food and Nutrition Research, 2020,91:275-300.
-
[6]
ZHAO X X,WANG Y R,CAI W C,et al. High-throughput sequencing-based analysis of microbial diversity in rice wine koji from different areas[J]. Current Microbiology,2020,77(5):882-889.
-
[7]
张清玫,刘芳,张建敏,等.基于人工合成菌群提升白酒品质的策略分析[J].酿酒科技,2024,8:71-77.
-
[8]
WANG C X, TANG J D, QIU S Y. Profiling of fungal diversity and fermentative yeasts in traditional Chinese Xiaoqu[J]. Frontiers in Microbiology,2020,11:2103.
-
[9]
HOU Q C, WANG Y R, NI H, et al. Deep sequencing reveals changes in prokaryotic taxonomy and functional diversity of pit muds in different distilleries of China[J]. Annals of Microbiology,2022,72(1):12.
-
[10]
NOURRISSON C,SCANZI J,BRUNET J,et al. Prokaryotic and eukaryotic fecal microbiota in irritable bowel syndrome patients and healthy individuals colonized with Blastocystis[J]. Frontiers in Microbiology,2021,12:713347.
-
[11]
LI Y L,LIU S P,ZHANG S Y,et al. Spatiotemporal distribution of environmental microbiota in spontaneous fermentation workshop:The case of Chinese Baijiu[J]. Food Research International,2022,156:111126.
-
[12]
XIAO R,CHEN S Q,WANG X Q,et al. Microbial community starters affect the profiles of volatile compounds in traditional Chinese Xiaoqu rice wine:Assement via highthroughput sequencing and gas chromatography-ion mobility spectrometry[J]. LWT-Food Science and Technology, 2022,170:114000.
-
[13]
CAI W C,WANG Y R,HOU Q C,et al. PacBio sequencing combined with metagenomic shotgun sequencing provides insight into the microbial diversity of Zha-chili[J]. Food Bioscience,2021,40:100884.
-
[14]
CHEN T Y,WANG H Y,SU W,et al. Analysis of the formation mechanism of volatile and non-volatile flavor substances in corn wine fermentation based on high-throughput sequencing and metabolomics[J]. Food Research International,2023,165:112350.
-
[15]
MVE-OBIANG A, MESTDAGH M, PORTAELS F. DNA isolation from chloroform/methanol-treated mycobacterial cells without lysozyme and proteinase K[J]. BioTechniques,2001,30(2):272-274,276.
-
[16]
WANG X H,WANG S H,ZHAO H. Unraveling microbial community diversity and succession of Chinese Sichuan sausages during spontaneous fermentation by highthroughput sequencing[J]. Journal of Food Science and Technology,2019,56(7):3254-3263.
-
[17]
唐佳代,邱树毅,王春晓,等.贵州地区酿酒小曲细菌多样性比较分析[J].中国酿造,2019,38(10):55-59.
-
[18]
ZHAO X X,LIU Y H,SHU L,et al. Study on metabolites of Bacillus producing soy sauce-like aroma in Jiang-flavor Chinese spirits[J]. Food Science&Nutrition, 2019, 8(1):97-103.
-
[19]
WADE M E,STRICKLAND M T,OSBORNE J P,et al. Role of Pediococcus in winemaking[J]. Australian Journal of Grape and Wine Research,2019,25(1):7-24.
-
[20]
ZHANG X R,LI Y X,ZHAO Y R,et al. Effect of Levilactobacillus brevis as a starter on the flavor quality of radish Paocai[J]. Food Research International,2023,168:112780.
-
[21]
向凡舒,朱媛媛,邓风,等.建始地区米酒曲细菌和真菌多样性研究[J].食品工业科技,2021,42(1):126-131.
-
[22]
梁敏华,赵文红,白卫东,等.白酒酒曲微生物菌群对其风味形成影响研究进展[J].中国酿造, 2023, 42(5):22-27.
-
[23]
XU P,WU Y J,CHEN H,et al. Promoting microbial community succession and improving the content of esters and aromatic compounds in strong-flavor Daqu via bioaugmentation inoculation[J]. Food Bioscience,2023,56:103299.
-
[24]
谢铃,刘双平,刘甜甜,等.辣蓼草对酒药关键微生物与品质的影响研究[J].食品与发酵工业, 2024, 50(23):53-61.
-
[25]
赵晨辉.馒头用酿酒酵母与乳酸菌的筛选及复合发酵剂的制备[D].阿拉尔:塔里木大学,2024.
-
[26]
王乃军,马美荣.扣囊复膜酵母在白酒生产中的应用研究进展[J].酿酒科技,2023,3:115-119.
-
[27]
雷炎,刘梦琦,易秦振,等.扣囊复膜酵母在红枣酒中的应用[J].食品研究与开发,2021,42(5):131-136.
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