JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

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Analysis of microbial diversity in meidicinal koji based on high-throughput sequencing and pure culture techniques

  • Corresponding author: WANG Yurong, wang_HFu6456@163.com
  • Received Date: 2024-09-05
    Accepted Date: 2025-02-27
    Available Online: 2025-08-27
  • MiSeq high-throughput sequencing and pure culture techniques were combined to analyze the microbial diversity of medicinal koji samples collected from Nanchang city, Jiangxi province, aiming to reveal the microbial composition within the koji. The results showed that the richness and diversity of bacterial taxa in the medicinal koji included in this study were significantly higher than those of the fungus. The dominant bacterial genera were Pediococcus and Levilactobacillus, with an average relative content of 38. 04% and 13. 00%, respectively. The dominant fungus genera were Candida and Saccharomycopsis, with an average relative content of 78. 30% and 17. 92%, respectively. Nine strains of bacteria isolated from medicinal koji were identified as three species: Pediococcus pentosaceus, Pediococcus acidilactici, and Bacillus licheniformis. Seven strains of fungus were identified as four species: Saccharomycopsis fibuligera, Saccharomycopsis malanga, Pichia kudriavzevii, and Saccharomyces cerevisiae.
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