7个福建茶树品种的遗传多样性分析及其分子指纹图谱构建
Analysis of genetic diversity of 7 tea plant varieties from Fujian and construction of their molecular fingerprints
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摘要: 基于SSR分子标记技术,对来自福建省的7个供试茶树品种的遗传多样性水平进行分析,并构建其分子指纹图谱.结果表明:初步筛选的14个SSR分子标记均为多态性标记,其多态性信息含量较丰富,可以较好地区分7个供试茶树品种;福云六号与福云七号供试茶树品种之间的遗传相似性系数最高,为8.06,亲缘关系最近;以扩增条带较少、多态性较高的3个SSR分子标记引物(PS3,PS15,PS19)相组合而构建的分子指纹图谱,可有效鉴别7个供试茶树品种,实现以最少的分子标记引物鉴定所有品种的目的.Abstract: Based on the SSR molecular marking technology, it analyzed the level of genetic diversity of 7 tested tea plant varieties from Fujian province and constructed the molecular fingerprint of them. The results showed that all of the 14 molecular markers primary screening were polymorphic markers with rich polymorphism information, and 7 tested tea plant varieties could be distinguished effectively. Genetic similarity coefficient between Fuyun No.6 and Fuyun No.7 was highest(8.06) which had a close phylogenetic relationship. 3 SSR molecular markers(PS3, PS15, PS19) were selected to construct fingerprint because of less amplified bands and higher Polymorphism, and the fingerprint could distinguish 7 tea plant varieties, achieved the purpose of identifying all varieties with the least molecular marker.
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Key words:
- tea plant /
- genetic diversity /
- molecular marking technology /
- molecular fingerprint
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