基于UHPLC法的巨豆三烯酮前体物同分异构体的定量分析及其在不同产地烟叶中的分布情况
Quantitative analysis of isomers of megastigmatrienone precursors based on UHPLC method and their distribution in tobacco leaves from various geographic origins
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摘要: 以烟叶中重要致香成分巨豆三烯酮前体物(3-氧代-α-紫罗兰醇-β-D-吡喃葡萄糖苷)两种同分异构体,即(6R, 9R)-3-氧代-α-紫罗兰醇-β-D-吡喃葡萄糖苷(rrOIPG)和(6R, 9S)-3-氧代-α-紫罗兰醇-β-D-吡喃葡萄糖苷(rsOIPG)为研究对象,建立超声辅助萃取-超高效液相色谱法对rrOIPG和rsOIPG进行定量分析,并利用该方法考查国内外7个产地烟叶中两种同分异构体的分布情况。结果表明:rrOIPG和rsOIPG分别在25.85~258.50 μg/mL(R2=0.999 1)和6.28~62.75 μg/mL(R2=0.999 0)内具有良好的线性关系,检出限分别为2.5 μg/mL和2.9 μg/mL,仪器精密度较高,样品的稳定性和重复性较好,加标回收率分别为83.94%和105.90%,RSD分别为1.22%和1.93%;根据rrOIPG和rsOIPG含量分布可较好地区分国内外产地烟叶,国外产地烟叶中rsOIPG含量明显高于国内产地烟叶,且国内外产地烟叶中rrOIPG的含量均高于rsOIPG。该方法可行性较好,前处理步骤简单,适用于烟叶中rrOIPG和rsOIPG的定量分析。
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关键词:
- 3-氧代-α-紫罗兰醇-β-D-吡喃葡萄糖苷 /
- 同分异构体 /
- 超高效液相色谱法 /
- 定量分析
Abstract: Investigating the quantitative analysis of two stereoisomeric compounds, specifically (6R, 9R)-3-oxo-α-ionol-β-D-glucopyranoside (rrOIPG) and (6R, 9S)-3-oxo-α-ionol-β-D-pyranoside (rsOIPG), megastigmatrienone precursors of the prominent aroma components in tobacco leaves, represented the core focus of this study. A meticulously-devised methodology combining ultrasound-assisted extraction with ultra-high-performance liquid chromatography (UHPLC) was deployed to ascertain the precise quantification of rrOIPG and rsOIPG. This robust analytical approach was judiciously employed to elucidate the geographical distribution patterns of these stereoisomers within tobacco leaves sourced from seven diverse global regions. The results revealed the compelling linear response characteristics of rrOIPG and rsOIPG, scanninged concentration ranges of 25.85 to 258.50 μg/mL (R2=0.999 1) and 6.28 to 62.75 μg/mL (R2=0.999 0), respectively. Exceptionally low detection limits of 2.5 μg/mL for rrOIPG and 2.9 μg/mL for rsOIPG underscore the method's inherent precision. The analysis exhibited superb stability and reproducibility, epitomized by recovery rates of 83.94% for rrOIPG and 105.90% for rsOIPG, and RSDs were 1.22% and 1.93%, respectively. According to the content distribution of rrOIPG and rsOIPG, the tobacco leaves from domestic and foreign origins could be well distinguished, and the content of rsOIPG configuration in foreign origins was significantly higher than that in domestic origins. In terms of the distribution of the two configurations, the content of rrOIPG configuration was higher than that of rsOIPG configuration. The developed technique exhibits high feasibility and streamlined pretreatment processes, making it well-suitable for the quantitative analysis of rrOIPG and rsOIPG in tobacco leaves. -
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