小黄姜精油提取工艺优化、成分分析及其生物活性研究

宋丽丽; 李科娜; 杨旭; 张志平; 马歌丽

doi:10.12187/2020.05.002

小黄姜精油提取工艺优化、成分分析及其生物活性研究

郑州轻工业大学食品与生物工程学院, 河南郑州 450001

作者简介: 宋丽丽(1987-),女,河南省信阳市人,郑州轻工业大学讲师,博士,主要研究方向为食品生物技术.;

基金项目: 国家自然科学基金项目（31801535）；郑州轻工业大学博士基金项目（2013BSJJ005）
中图分类号: TS201.1

Study on the optimization of extraction process,composition analysis and biological activity of essential oil from Zingiber officinale Roscoe

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China
Received Date: 2020-05-11

CLC number: TS201.1

摘要: 以小黄姜为原料，采用同时蒸馏萃取法提取小黄姜精油，通过单因素试验和正交试验获得小黄姜精油的最佳提取工艺，利用GC-MS分析其化学成分，并对其抗氧化活性和抑菌活性进行研究.结果表明：小黄姜精油的最佳提取工艺条件为料液比1： 24，蒸馏时间2.5 h，浸泡时间1.5 h，在此条件下，小黄姜精油的提取率为2.70%；小黄姜精油中共检测出58种化学成分，相对含量在0.50%以上的挥发性化学成分有20种，其中，姜烯含量最高，为27.14%，其次是β-倍半水芹烯、α-金合欢烯和β-水芹烯；小黄姜精油对ABTS自由基和DPPH自由基均具有较强的清除能力，其IC 50分别为0.450 5 mg/mL和0.703 7 mg/mL；与市售姜精油相比，小黄姜精油显示出更强的抑菌性能，在抑制大肠杆菌（革兰氏阴性菌）方面更具优势.
- 小黄姜 /
- 精油 /
- 同时蒸馏萃取 /
- 抗氧化活性 /
- 抑菌活性
Abstract: Zingiber officinale Roscoe was used as materials to extracting the essential oil by simultaneous-distillation and solvent extraction.Single factor test and orthogonal test were carried out to obtain optimal conditions.The composition of essential oils was analyzed by GC-MS,and antibacterial activity and antioxidant activity of the essential oil were further studied.Results showed that the optimal process conditions of Zingiber officinale Roscoe essential oil were 1:24 solid-liquid ration,2.5 h extraction time and 1.5 h soaking time,and the yield of essential oil was 2.70%.A total of 58 chemical components were detected in the essential oil of Zingiber officinale Roscoe,and there were 20 kinds of volatile chemical components with a relative percentage of more than 0.50%.Among them,the content of Zingiberene was the highest,which was 27.14%,followed by β-sesquiphellandrene,α-faresene and β-phellandrene.Meanwhile,essential oil had significant scavenging effect on ABTS radicals and DPPH radicals,the IC 50 were 0.450 5 mg/mL and 0.703 7mg/mL,respectively.Compared with the commercial ginger essential oil,the Zingiber officinale Roscoe essential oil had stronger antibacterial effect,which had more advantages in inhibiting Escherichia coli(Gram-negative bacteria).
- Zingiber officinale Roscoe /
- essential oil /
- simultaneous-distillation and solvent extraction /
- antioxidant activity /
- antibacterial activity
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沈阳化工大学材料科学与工程学院沈阳 110142

小黄姜精油提取工艺优化、成分分析及其生物活性研究

作者简介: 宋丽丽(1987-),女,河南省信阳市人,郑州轻工业大学讲师,博士,主要研究方向为食品生物技术.;

Study on the optimization of extraction process,composition analysis and biological activity of essential oil from Zingiber officinale Roscoe

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通讯作者: 陈斌, bchen63@163.com

小黄姜精油提取工艺优化、成分分析及其生物活性研究

作者简介:宋丽丽(1987-),女,河南省信阳市人,郑州轻工业大学讲师,博士,主要研究方向为食品生物技术.

English Abstract

Study on the optimization of extraction process,composition analysis and biological activity of essential oil from Zingiber officinale Roscoe

目录

小黄姜精油提取工艺优化、成分分析及其生物活性研究

作者简介: 宋丽丽(1987-),女,河南省信阳市人,郑州轻工业大学讲师,博士,主要研究方向为食品生物技术.;

Study on the optimization of extraction process,composition analysis and biological activity of essential oil from Zingiber officinale Roscoe

计量

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通讯作者: 陈斌, bchen63@163.com

小黄姜精油提取工艺优化、成分分析及其生物活性研究

作者简介:宋丽丽(1987-),女,河南省信阳市人,郑州轻工业大学讲师,博士,主要研究方向为食品生物技术.

English Abstract

Study on the optimization of extraction process,composition analysis and biological activity of essential oil from Zingiber officinale Roscoe

目录