产香菌株的分离鉴定及其发酵产物在卷烟加香中的应用
Isolation and identification of aroma-producing strains and application of fermentation products in cigarette flavoring
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摘要: 以阳光玫瑰葡萄表皮为微生物分离源,筛选分离得到一株产香菌株MG6,对该菌株进行鉴定并分析其发酵液的特征香气成分,进而利用该菌株制备烟用香料并进行卷烟加香评价.结果表明:该菌株为陆生毕赤酵母(Pichia terricola);与对照组相比,该菌株发酵液中酮类、醇类、酯类、酚类、烯烃类等烟叶主要特征香气成分均明显提高,其中巨豆三烯-3-酮、二氢猕猴桃内酯、新植二烯的总体含量均有不同程度的提高,这些物质对于提升烟草的香气质量非常重要;当烟用香料添加量为0.05%时,卷烟香气质和香气量均有所提高,刺激性明显降低,整体感官品质提升.Abstract: An aroma-producing strain MG6 with aroma production was isolated from the surface of sunny rose grape. The strain was identified and the characteristic aroma components of its fermentation broth were analyzed. Then the tobacco flavor was prepared by the strain and the aroma evaluation of cigarette was carried out. The results showed that the strain was Pichia terricola. Compared with the control group, the main characteristic aroma components such as ketones, alcohols, esters, phenols and olefins in the fermentation broth of the strain were significantly increased, and the total contents of megastigmatrien-3-one, dihydrokiwifruit lactone and neophytadiene were increased in different degrees, which were particularly important for improving the aroma quality of tobacco. When the added amount of tobacco flavoring was 0. 05%, the quality and amount of aroma were improved, the irritation was reduced, and the overall sensory quality was improved.
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Key words:
- aroma-producing strain /
- isolation /
- identification /
- aroma component /
- tobacco flavor
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