群体感应淬灭酶对MBR-CANON工艺脱氮性能及污泥特性的影响

张肖静; 张红丽; 张楠; 位登辉; 马冰冰; 张涵; 杨浩洁

doi:10.12187/2022.06.014

群体感应淬灭酶对MBR-CANON工艺脱氮性能及污泥特性的影响

郑州轻工业大学环境污染治理与生态修复河南省协同创新中心, 河南郑州 450001

作者简介: 张肖静(1986—),女,河南省开封市人,郑州轻工业大学副教授,博士,主要研究方向为环境污染治理新技术。E-mail:zhang-xiaojing@zzuli.edu.cn;

基金项目: 国家自然科学基金项目(41701569)
河南省高校科技创新人才支持计划项目(20HASTIT014)
中图分类号: X703.1

Effect of quorum quenching enzyme on the nitrogen removal performance and sludge property of MBR-CANON process

He'nan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou 450001, China
Received Date: 2021-08-13
Accepted Date: 2022-05-06

CLC number: X703.1

摘要: 在稳定运行的基于膜生物反应器的全程自养脱氮工艺过程中投加淬灭酶-酰基高丝氨酸内酯(Acyl-Homoserine-Lactones,AHLs)酰基转移酶,跟踪分析该工艺过程中的脱氮性能、污泥性能、关键酶活及功能微生物组成的变化情况。结果表明,AHLs酰基转移酶的加入导致好氧氨氧化菌的活性严重下降,当AHLs酰基转移酶质量浓度为0.5 mg/L时,氨氮去除率从最初的83.7%下降至57.0%,总氮去除率由65.9%下降至43.6%,但AHLs酰基转移酶可大幅降低膜丝表面附着的污泥中胞外聚合物含量,有利于减缓膜污染;AHLs酰基转移酶的加入导致自养脱氮关键酶—羟氨氧化还原酶、氨单加氧酶及亚硝酸盐还原酶的活性下降。AHLs酰基转移酶对好氧氨氧化菌的活性抑制大于厌氧氨氧化菌,但对两者相对丰度的影响则相反,厌氧氨氧化菌受到的抑制主要表现为基质不足导致的生长受限。
- 群体感应 /
- 淬灭酶 /
- 自养脱氮 /
- 胞外聚合物 /
- 膜生物反应器 /
- 关键酶活
Abstract: Quorum quench enzyme-Acyl-Homoserine-Lactones (AHLs) acyltransferase was added to the stable MBR-CANON process, the nitrogen removal performance, sludge property, key enzymes' activity and microbial community were tracked and analyzed. The results showed that the addition of AHLs acyltransferase significantly suppressed the nitrogen removal ability. The AHLs acyltransferase in 0.5 mg/L obviously decreased the ammonia removal efficiency from 83.7% to 57.0%, and the total nitrogen removal efficiency from 65.9% to 43.6%. The extracellular polymeric substance in the sludge attaching to the membrane surface was also decreased by the AHLs acyltransferase, which was beneficial to alleviate the membrane fouling. Additionally, the activity of hydroxylamine oxidoreductase, ammonia monooxygenase, and nitrite reductase all significantly decreased with the addition of AHLs acyltransferase. The inhibition of AHLS acyltranferase on the activity of aerobic ammonia-oxidizing bacteria was greater than that on the anaerobic ammonia-oxidizing bacteria (AAOB), but the effect on their relative abundances was opposite. The suppression on AAOB was mainly attributed to the lack of substrate.
- quorum sensing /
- quenching enzyme /
- autotrophic nitrogen removal /
- extracellular polymeric substance /
- membrane bioreactor /
- enzyme activity
1. [1]
  ARORAA A S,NAWAZ A,QYYUMA M A,et al.Energy saving anammox technology-based nitrogen removal and bioenergy recovery from wastewater:Inhibition mechanisms,state-of-the-art control strategies,and prospects[J].Renewable and Sustainable Energy Reviews,2021,135:110126.
2. [2]
  WU D,LI X Z,LI X D.Toward energy neutrality in municipal wastewater treatment:A systematic analysis of energy flow balance for different scenarios[J].ACS ES&T Water,2021,1(4):796-807.
3. [3]
  张肖静,张涵,周月,等.亚硝化-厌氧氨氧化工艺的启动及微生物种群演替规律研究[J].轻工学报,2019,34(6):54-63.
4. [4]
  ZHANG X L,LIU X C,ZHANG M.Performance and microbial community of the CANON process in a sequencing batch membrane bioreactor with elevated COD/N ratios[J].Water Science & Technology,2020,81(2):138-147.
5. [5]
  MAO X,MYAVAGH P H,LOTFIKATOULI S,et al.Membrane bioreactors for nitrogen removal from wastewater:A review[J].Journal of Environmental Engineering,2020,146(5):03120002.
6. [6]
  VAN DER STAR W R L,MICLEA A I,VAN DONGEN U G J M,et al.The membrane bioreactor:A novel tool to grow anammox bacteria as free cells[J].Biotechnology & Bioengineering,2010,101(2):286-294.
7. [7]
  WANG S,ZOU L X,LI H B,et al.Full-scale membrane bioreactor process WWTPs in East Taihu basin:Wastewater characteristics,energy consumption and sustainability[J].Science of the Total Environment,2020,723:137983.
8. [8]
  ZHOU J H,JIANG S F,YU H C,et al.A comparative study on membrane fouling alleviation mechanisms by using nanoscale Fe₃O₄ and poly dimethyldiallylammonium chloride[J].Environmental Technology,2020,41(12):1477-1485.
9. [9]
  DU X,SHI Y,JEGATHEESAN V,et al.A review on the mechanism,impacts and control methods of membrane fouling in MBR system[J].Membranes,2020,10(2):24.
10. [10]
  MUTLU B K,ERGÖN-CAN T,KOYUNCU I,et al.Quorum quenching for effective control of biofouling in membrane bioreactor:A comprehensive review of approaches,applications,and challenges[J].Environmental Engineering Research,2019,24(4):543-558.
11. [11]
  KHAN M,KHAN S J,HASAN S W.Quorum sensing control and wastewater treatment in quorum quenching/submerged membrane electro-bioreactor (SMEBR(QQ)) hybrid system[J].Biomass & Bioenergy,2019,128:105329.
12. [12]
  张泽瀚,洪乾坤,刘博超,等.群体猝灭技术在控制MBR生物污染中的研究进展[J].工业水处理,2021,41(4):7-13.
13. [13]
  LEE K,YU H R,ZHANG X L,et al.Quorum sensing and quenching in membrane bioreactors:Opportunities and challenges for biofouling control[J].Bioresource Technology,2018,270:656-668.
14. [14]
  常晶,史国萃,曾名湧,等.细菌群体感应淬灭酶及其应用研究进展[J].生物加工过程,2019,17(3):244-250.
15. [15]
  ZHANG X J,WEI D H,CHEN Z,et al.Impact of increasing sulfide addition on the nitrogen removal and microbial community of CANON process in membrane bioreactor[J].Biochemical Engineering Journal,2021,173:108071.
16. [16]
  ENSIGN S A,HYMAN M R,ARP D J.In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper[J].Journal of Bacteriology,1993,175(7):1971-1980.
17. [17]
  SLIEKERS A O,DERWORT N,GOMEZ J,et al.Completely autotrophic nitrogen removal over nitrite in one single reactor[J].Water Research,2002,36(10):2475-2482.
18. [18]
  张肖静,傅浩强,张楠,等.低基质厌氧氨氧化滤柱的快速启动及稳定运行[J].轻工学报,2018,33(4):42-49.
19. [19]
  YU H R, QU F S,ZHANG X L,et al.Effect of quorum quenching on biofouling and ammonia removal in membrane bioreactor under stressful conditions[J].Chemosphere:Environmental Toxicology and Risk Assessment,2018,199:114-121.
20. [20]
  PALUCH E,REWAK-SOROCZYN＇SKA J,JDRUSIK I,et al.Prevention of biofilm formation by quorum quenching[J].Applied Microbiology and Biotechnology,2020,104(1):1871-1881.
21. [21]
  LIU Y E,SUN J,PENG L,et al.Assessment of heterotrophic growth supported by soluble microbial products in anammox biofilm using multidimensional modeling[J].Scientific Reports,2016,6(1):275-276.
22. [22]
  何志仙,魏旖旎,刘婧晶,等.活性污泥羟氨氧化还原酶粗酶提取及活性测定方法的优化研究[J].环境科学学报,2015,35(12):3797-3804.
23. [23]
  YIN X,QIAO S,YU C,et al.Effects of reduced graphene oxide on the activities of anammox biomass and key enzymes[J].Chemical Engineering Journal,2015,276:106-112.
24. [24]
  OUYANG Y,HU Y,HUANG J H,et al.Effects of exogenous quorum quenching on microbial community dynamics and biofouling propensity of activated sludge in MBRs[J].Biochemical Engineering Journal,2020,157:107534.
25. [25]
  SALEHIZIRI M,AMALFITANO S,GALLIPOLI A,et al.Investigating the influences of quorum quenching and nutrient conditions on activated sludge flocs at a short-time scale[J].Chemosphere,2020,248:125917.
26. [26]
  WAHEED H,XIAO Y Y,HASHMI I,et al.The selective pressure of quorum quenching on microbial communities in membrane bioreactors[J].Chemosphere,2020,247:125953.
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群体感应淬灭酶对MBR-CANON工艺脱氮性能及污泥特性的影响

作者简介: 张肖静(1986—),女,河南省开封市人,郑州轻工业大学副教授,博士,主要研究方向为环境污染治理新技术。E-mail:zhang-xiaojing@zzuli.edu.cn;

Effect of quorum quenching enzyme on the nitrogen removal performance and sludge property of MBR-CANON process

计量

通讯作者: 陈斌, bchen63@163.com

群体感应淬灭酶对MBR-CANON工艺脱氮性能及污泥特性的影响

作者简介:张肖静(1986—),女,河南省开封市人,郑州轻工业大学副教授,博士,主要研究方向为环境污染治理新技术。E-mail:zhang-xiaojing@zzuli.edu.cn

English Abstract

Effect of quorum quenching enzyme on the nitrogen removal performance and sludge property of MBR-CANON process

目录

群体感应淬灭酶对MBR-CANON工艺脱氮性能及污泥特性的影响

作者简介: 张肖静(1986—),女,河南省开封市人,郑州轻工业大学副教授,博士,主要研究方向为环境污染治理新技术。E-mail:zhang-xiaojing@zzuli.edu.cn;

Effect of quorum quenching enzyme on the nitrogen removal performance and sludge property of MBR-CANON process

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通讯作者: 陈斌, bchen63@163.com

群体感应淬灭酶对MBR-CANON工艺脱氮性能及污泥特性的影响

作者简介:张肖静(1986—),女,河南省开封市人,郑州轻工业大学副教授,博士,主要研究方向为环境污染治理新技术。E-mail:zhang-xiaojing@zzuli.edu.cn

English Abstract

Effect of quorum quenching enzyme on the nitrogen removal performance and sludge property of MBR-CANON process

目录