一株高耐受粗甘油枯草芽孢杆菌的分子机制研究

贾晨阳; 张帆; 刘兰茜; 王光路; 杨雪鹏

doi:10.12187/2025.05.006

一株高耐受粗甘油枯草芽孢杆菌的分子机制研究

贾晨阳 ¹ ,
张帆 ¹ ,
刘兰茜 ² ,
王光路 ¹ ,
杨雪鹏 ^{1

,

,}

1. 郑州轻工业大学烟草科学与工程学院, 河南郑州 450001;
2. 江西中烟工业有限责任公司井冈山卷烟厂, 江西吉安 343100

作者简介: 贾晨阳(2000—),男,山东省滨州市人,郑州轻工业大学硕士研究生,主要研究方向为生物技术与工程。E-mail:1765636995@qq.com;

通讯作者: 杨雪鹏,yangxuepeng@zzuli.edu.cn

基金项目: 河南省科技攻关重点研发与推广专项项目(232102311136)
河南省自然科学基金重点项目(242300421202)
中图分类号: TS201.3

Investigation into the molecular mechanism of a Bacillus subtilis strain exhibiting high tolerance to crude glycerol

1. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. Jinggangshan Cigarette Factory, China Tobacco Jiangxi Industry Co., Ltd., Ji'an 343100, China

Corresponding author: YANG Xuepeng, yangxuepeng@zzuli.edu.cn

Received Date: 2025-01-02
Accepted Date: 2025-05-12

CLC number: TS201.3

摘要: 选取高耐受粗甘油的枯草芽孢杆菌为研究对象,利用全基因组和转录组测序技术解析其对高质量浓度粗甘油的耐受机制。结果表明:进化菌株中检测到23个突变基因,涉及ABC转运系统、烟酸与烟酰胺代谢、群体感应、芽孢生成等关键途径。相比出发菌株,进化菌株在甘油(酯)代谢、三羧酸循环、嘌呤代谢、ABC转运系统、能量代谢等途径中的基因表达显著上调,脂肪酸和生物素合成代谢也明显增强;部分丧失芽孢生成能力,稳定中期和后期的芽孢生成率分别下降了38.0%和52.3%;细胞膜新增6种成分,脂肪酸种类和质量浓度均显著增加,磷脂双分子层稳定性增强,这是其高粗甘油耐受性和快速生长的关键原因。
- 枯草芽孢杆菌 /
- 粗甘油耐受 /
- 分子机制解析 /
- 全基因组测序 /
- 转录组测序
Abstract: The Bacillus subtilis strain with high tolerance to crude glycerol was selected as the research subject, and its tolerance mechanism to high-concentration crude glycerol was investigated using whole genome and transcriptome sequencing technologies. The results showed that 23 mutantgenes were identified in the evolved strain, involving key pathways such as the ABC transport system, nicotinic acid and nicotinamide metabolism, quorum sensing, and spore formation. Compared to the parent strain, the evolved strain exhibited significantly upregulated gene expression in pathways related to glycerol (ester) metabolism, the tricarboxylic acid cycle, purine metabolism, the ABC transport system, and energy metabolism. The synthesis metabolism of fatty acids and biotin of the evolved strainwere markedly enhanced. Furthermore, the evolved strain partially lost its spore-forming capacity, with spore formation rates decreasing by 38.0% and 52.3% during the stable middle and late stages, respectively. Additionally, six new components were detected in the cell membrane, along with a significant increase in both the types and concentration of fatty acids, thereby enhancing the stability of the phospholipid bilayer. This enhancement was the critical factor contributing to the strain’s high tolerance to crude glycerol and its rapid growth.
- Bacillus subtilis /
- crude glycerol tolerance /
- analysis of molecular mechanisms /
- whole genome sequencing /
- RNA-sequencing
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一株高耐受粗甘油枯草芽孢杆菌的分子机制研究

作者简介: 贾晨阳(2000—),男,山东省滨州市人,郑州轻工业大学硕士研究生,主要研究方向为生物技术与工程。E-mail:1765636995@qq.com;

通讯作者: 杨雪鹏,yangxuepeng@zzuli.edu.cn

Investigation into the molecular mechanism of a Bacillus subtilis strain exhibiting high tolerance to crude glycerol

Corresponding author: YANG Xuepeng, yangxuepeng@zzuli.edu.cn

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通讯作者: 陈斌, bchen63@163.com

一株高耐受粗甘油枯草芽孢杆菌的分子机制研究

作者简介:贾晨阳(2000—),男,山东省滨州市人,郑州轻工业大学硕士研究生,主要研究方向为生物技术与工程。E-mail:1765636995@qq.com

通讯作者: 杨雪鹏, yangxuepeng@zzuli.edu.cn

English Abstract

Investigation into the molecular mechanism of a Bacillus subtilis strain exhibiting high tolerance to crude glycerol

Corresponding author: YANG Xuepeng, yangxuepeng@zzuli.edu.cn

目录

一株高耐受粗甘油枯草芽孢杆菌的分子机制研究

作者简介: 贾晨阳(2000—),男,山东省滨州市人,郑州轻工业大学硕士研究生,主要研究方向为生物技术与工程。E-mail:1765636995@qq.com;

通讯作者: 杨雪鹏,yangxuepeng@zzuli.edu.cn

Investigation into the molecular mechanism of a Bacillus subtilis strain exhibiting high tolerance to crude glycerol

Corresponding author: YANG Xuepeng, yangxuepeng@zzuli.edu.cn

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通讯作者: 陈斌, bchen63@163.com

一株高耐受粗甘油枯草芽孢杆菌的分子机制研究

作者简介:贾晨阳(2000—),男,山东省滨州市人,郑州轻工业大学硕士研究生,主要研究方向为生物技术与工程。E-mail:1765636995@qq.com

通讯作者: 杨雪鹏, yangxuepeng@zzuli.edu.cn

English Abstract

Investigation into the molecular mechanism of a Bacillus subtilis strain exhibiting high tolerance to crude glycerol

Corresponding author: YANG Xuepeng, yangxuepeng@zzuli.edu.cn

目录