马铃薯蛋白-黄原胶微凝胶的制备及其乳化特性研究
Study on preparation and emulsion property of potato protein-xanthan gum microgel
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摘要: 以马铃薯蛋白(PP)和黄原胶(XG)为原料,通过研究不同pH值、热处理条件和PP/XG质量比确定马铃薯蛋白-黄原胶微凝胶(PP-XGM)的最佳制备条件,对该条件下制备的PP-XGM结构进行表征,以动力学稳定性指数(TSI)为指标对其乳化稳定性进行研究.结果表明:PP-XGM的最佳制备条件为PP/XG质量比1:1,pH值3.0,80℃热处理30 min;与PP-XG相比,PP-XGM的粒径更大,多分散系数更小,颗粒分布更均匀;与PP相比,PP-XG和PP-XGM的最大吸收波长λmax发生了不同程度的蓝移,且PP-XGM的荧光强度(FI)下降最显著;采用透射电镜发现PP-XGM呈核壳结构;在油相体积分数相同的条件下,PP-XGM的乳化稳定性较PP显著提高.Abstract: Potato protein (PP) and xanthan gum (XG) were used as raw materials.The optimal preparation conditions of xanthan gum microgel (PP-XGM) were determined through different pH value,heat treatment conditions,and potato protein/xanthan gum mass ratio.The structure of the PP-XGM prepared under this condition was characterized.The stability kinetic index was used as an indicator to study its emulsification stability.The results showed that the best preparation conditions for PP-XGM were PP/XG mass ratio of 1:1,pH value 3.0,and 80℃ water bath heating for 30 min.Compared with PP-XG,PP-XGM had larger particle size,smaller polydispersity coefficient and more uniform particle distribution.Compared with PP,the maximum absorption wavelength λmax of PP-XG and PP-XGM had a different degree of blue shift,and the fluorescence intensity FI of PP-XGM had the most significant decrease.Using projection electron microscopy,it was found that PP-XGM had a core-shell structure.Under the same oil phase volume fraction,the emulsion stability of PP-XGM was significantly higher than that of PP.
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Key words:
- potato protein /
- xanthan gum /
- microgel /
- structure characterization /
- emulsion property
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[1]
DAVID S,LIVNEY Y D.Potato protein based nanovehicles for health promoting hydrophobic bioactives in clear beverages[J].Food Hydrocolloids,2016,57:229.
-
[2]
WAGLAY A,KARBOUNE S.Advances in potato chemistry and technology[M].2nd ed.London:Academic Press,2016:75.
-
[3]
WANG C J,TONG C,ZHANG D L,et al.Preparation and characterization of potato protein-based microcapsules with an emphasis on the mechanism of interaction among the main components[J].Journal of the Science of Food and Agriculture,2020,100:2866.
-
[4]
SCHMIDT J M,DAMGAARD H,GREVE-POULSEN M,et al.Foam and emulsion properties of potato protein isolate and purified fractions[J].Food Hydrocolloids,2018,74:367.
-
[5]
MCCLEMENTS D J.Designing biopolymer microgels to encapsulate,protect and deliver bioactive components:Physicochemical aspects[J].Advances in Colloid and Interface Science,2017,240:31.
-
[6]
陈鸿强,仇丹,郑红霞,等.食品微凝胶的制备、表征与应用[J].中国食品添加剂,2019(12):171.
-
[7]
YIN B,DENG W,XU K.Stable nano-sized emulsions produced from soy protein and soy polysaccharide complexes[J].Journal of Colloid and Interface Science,2012,380(1):51.
-
[8]
TRAN T,ROUSSEAU D.Stabilization of acidic soy protein-based dispersions and emulsions by soy soluble polysaccharides[J].Food Hydrocolloids 2013,30:382.
-
[9]
-
[10]
夏晓凤.大豆可溶性多糖对大豆蛋白凝胶的影响及机理初探[D].无锡:江南大学,2015.
-
[11]
MAO L K,YANG J,XU D X,et al.Effects of homogenization models and emulsifiers on the physicochemical properties of β-carotene nanoemulsions[J].Journal of Dispersion Science and Technology,2010,31(7):986.
-
[12]
丁徐哲.植物蛋白与大豆多糖纳米凝胶的制备及其应用研究[D].上海:复旦大学,2013.
-
[13]
孙莹,魏冬旭,姚春艳,等.不同处理条件对马铃薯糖蛋白Patatin构象的影响研究[J].食品工业科技,2017(17):86.
-
[14]
刘振艳,徐红华.热致乳蛋白微米凝胶的特性[J].中国乳品工业,2012,40(11):7.
-
[15]
李兴飞.大豆乳清蛋白与多糖的复合作用以及蛋白质组分的选择性提[D].无锡:江南大学,2017.
-
[16]
甘招娣.米糠清蛋白-壳聚糖纳米自组装包载姜黄素纳米体系制备与评价[D].南昌:南昌大学,2017.
-
[17]
JOURDAIN L,LESE M E,SCHMITT C,et al.Stability of emulsions containing sodium caseinate and dextran sulfate:Relationship to complexation in solution[J].Food Hydrocolloids,2008,22(4):647.
-
[18]
袁杨.食物蛋白与壳聚糖相互作用及其在食品体系的应用研究[D].广州:华南理工大学,2014.
-
[19]
WANG K Q,LUO S Z,ZHONG X Y,et al.Changes in chemical interactions and protein conformation during heat-induced wheat gluten gel formation[J].Food Chemistry,2017,214:393.
-
[20]
WANG B,WANG L J,LI D,et al.Effects of protein concentration and oil-phase volume fraction on the stability and rheology of menhaden oil-in-water emulsions stabilized by whey protein isolate with xanthan gum[J].Food Hydrolloids,2009,23(1):165.
-
[21]
LIU F,TANG C H.Soy protein nanoparticle aggregates as pickering stabilizers for oil-in-water emulsions[J].Journal of Agricultural and Food Chemistry,2013,61(37):8888.
-
[22]
王金梅.大豆蛋白热聚集行为及界面、乳化性质研究[D].广州:华南理工大学,2012.
-
[23]
冯洁茹,张鑫浩,赵春进,等.蛋白质-多糖在乳液油-水界面相互作用研究进展[J].发酵科技通讯,2019,48(1):14.
-
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