艾草多糖结构特征及抗氧化活性研究

王子朝; 舒志涵; 郑毅; 陈庆功

doi:10.12187/2026.05.002

艾草多糖结构特征及抗氧化活性研究

1. 河南工业大学生物工程学院, 河南郑州 450001;
2. 焦作市昊仕达农业有限公司, 河南焦作 454950

作者简介: 王子朝(1985—),男,河南省林州市人,河南工业大学副教授,博士,主要研究方向为糖化学与糖生物学。E-mail:6832917@163.com;

基金项目: 河南省重大科技专项项目(231100110300)
中图分类号: TS201.1

Structural characterization and antioxidant activity of Artemisia argyi polysaccharids

1. School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China;
2. Jiaozuo Haoshida Agriculture Co., Ltd., Jiaozuo 454950, China
Received Date: 2024-08-07
Accepted Date: 2026-09-29

CLC number: TS201.1

摘要: 【目的】解析艾草多糖的结构特征及抗氧化活性,促进艾草资源高值化利用及天然抗氧化功能因子开发。【方法】采用水提醇沉法提取艾草多糖,利用高效液相色谱和波谱分析艾草多糖组成和结构特征;通过DPPH、超氧阴离子、羟基和ABTS⁺自由基清除实验评价其体外抗氧化活性,并通过H₂O₂诱导的小鼠单核巨噬细胞系RAW264.7氧化损伤模型测定其氧化修复作用。【结果】艾草多糖由葡萄糖、半乳糖、阿拉伯糖、甘露糖、木糖和鼠李糖组成,物质的量比为1.00∶0.26∶0.23∶0.19∶0.15∶0.13,重均分子质量和多分散系数分别为25 934 Da和1.211;在0.31~5.00 mg/mL质量浓度范围内,艾草多糖对DPPH、超氧阴离子、羟基和ABTS⁺自由基的清除能力呈质量浓度依赖性,其中ABTS⁺自由基清除率最高达99.68%,与抗坏血酸(VC)相当;艾草多糖对RAW264.7细胞无明显毒性,且在H₂O₂诱导的氧化损伤模型中,使总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力分别从0.17 U/mg prot、1.21 U/mg prot、4.90 U/mg prot提升至0.33 U/mg prot、1.58 U/mg prot、8.88 U/mg prot,丙二醛(MDA)水平从15.92 nmol/mL降低至8.89 nmol/mL,呈浓度依赖性。【结论】所得艾草多糖具有显著体外及细胞内抗氧化活性,可通过增强抗氧化酶活性、减轻脂质过氧化发挥修复作用,具备开发为天然抗氧化剂的潜力。
- 艾草多糖 /
- 结构特征 /
- 抗氧化活性 /
- 细胞氧化修复作用
Abstract: 【Objective】This study aimed to analyze the structural characteristics and antioxidant activity of Artemisia argyi polysaccharides, thereby facilitating the high-value utilization of Artemisia argyi resources and the development of natural antioxidant functional ingredients.【Methods】Artemisia argyi polysaccharides were extracted by water extraction and ethanol precipitation. High-performance liquid chromatography and spectroscopic analysis were used to analyze their composition and structural characteristics. The in vitro antioxidant activity was evaluated by DPPH, superoxide anion, hydroxyl, and ABTS⁺ radical scavenging assays. The cellular oxidative damage repair effect was assessed using an H₂O₂-induced oxidative damage model in the murine monocyte/macrophage cell line RAW264.7.【Results】The polysaccharides consisted of glucose, galactose, arabinose, mannose, xylose, and rhamnose at a molar ratio of 1.00∶0.26∶0.23∶0.19∶0.15∶0.13. Their weight-average molecular weight and polydispersity index were 25 934 Da and 1.211, respectively. At mass concentrations of 0.31~5.00 mg/mL, the scavenging capacities of the polysaccharides against DPPH, superoxide anion, hydroxyl, and ABTS⁺ radicals were concentration-dependent. The maximum ABTS⁺radical scavenging rate reached 99.68%, which was comparable to that of ascorbic acid (VC). The polysaccharides showed no obvious cytotoxicity toward RAW264.7 cells. In the H₂O₂-induced oxidative damage model, the polysaccharides increased T-SOD, CAT, and GSH-Px activities from 0.17 U/mg protein, 1.21 U/mg protein, and 4.90 U/mg protein to 0.33 U/mg protein, 1.58 U/mg protein, and 8.88 U/mg protein, respectively, and decreased the MDA level from 15.92 nmol/mL to 8.89 nmol/mL in a concentration-dependent manner.【Conclusion】The obtained Artemisia argyi polysaccharides exhibited significant in vitro and intracellular antioxidant activity, exerting oxidative repair effects by enhancing antioxidant enzyme activities and reducing lipid peroxidation, thus indicating their potential for development as natural antioxidants.
- Artemisia argyi polysaccharide /
- structural characterization /
- antioxidant activity /
- cellular oxidation repair effect
1. [1]
  Wang Z C,Zhou X Y,Chang H,et al.Antioxidant analysis of flavonoids extracted from Artemisia argyi leaf and their antibacterial activities against food-borne pathogens Escherichia coli and Staphylococcus aureus[J].Biologia,2024,79(3):975-983.
2. [2]
  Wang Z C,Wang L,Huang H T,et al.In vitro antioxidant analysis of flavonoids extracted from Artemisia argyi stem and their anti-inflammatory activity in lipopolysaccharide-stimulated RAW264.7 macrophages[J].Food Chemistry,2023,407:135198.
3. [3]
  Song Q Y,Gu L B,Wu H K,et al.Chemical structure and ACE inhibitory activity of polysaccharide from Artemisia vulgaris L[J].Journal of Molecular Structure,2022,1250:131896.
4. [4]
  王苗苗.艾草不同部位效应成分及药理作用研究[D].郑州:河南工业大学,2024. Wang M M.Study on the effective components and pharmacological effects in different parts of Artemisia argyi[D].Zhengzhou:Henan University of Technology,2024.
5. [5]
  何柳,王云鹏,谢卫红,等.艾叶多糖的提取、结构分析与功能的研究进展[J].食品研究与开发,2022,43(1):202-209.
  He L,Wang Y P,Xie W H,et al.A review of the extraction,structure and function of Artemisia argyi polysaccharides[J].Food Research and Development,2022,43(1):202-209.
6. [6]
  舒志涵,胡茜蕾,张沛瑶,等.益生菌发酵对植物多糖影响的研究进展[J].河南工业大学学报(自然科学版),2025,46(2):133-143.
  Shu Z H,Hu X L,Zhang P Y,et al.Research progress on the effect of probiotic fermentation on plant-based polysaccharides[J].Journal of Henan University of Technology (Natural Science Edition),2025,46(2):133-143.
7. [7]
  周雪艳,常贺,苟海芹,等.分子量影响多糖生物活性的研究进展[J].河南工业大学学报(自然科学版),2024,45(1):124-132.
  Zhou X Y,Chang H,Gou H Q,et al.Advances in research of molecular weight affecting the bioactivities of polysaccharides[J].Journal of Henan University of Technology (Natural Science Edition),2024,45(1):124-132.
8. [8]
  王子朝,郑欣欣,余晓雪,等.多糖对食品物性及功能影响的研究进展[J].河南工业大学学报(自然科学版),2023,44(1):126-134.
  Wang Z C,Zheng X X,Xu X X,et al.Research progress on the effect of polysaccharide on physical property and function of food[J].Journal of Henan University of Technology (Natural Science Edition),2023,44(1):126-134.
9. [9]
  王子朝,张迪,盛丽丽,等.超声降解影响多糖理化特性和生物活性研究进展[J].河南工业大学学报(自然科学版),2021,42(6):121-126.
  Wang Z C,Zhang D,Sheng L L,et al.Research advances of ultrasound degradation on physicochemical property and biological activity of polysaccharides[J].Journal of Henan University of Technology (Natural Science Edition),2021,42(6):121-126.
10. [10]
  Zhang P,Yang D,Xiao J,et al.Artemisia argyi polysaccharide alleviates osmotic diarrhea by enhancing intestinal barrier protection and anti-inflammation[J].International Journal of Biological Macromolecules,2024,282:136779.
11. [11]
  Zhu L,Gong H,Gan X,et al.“Processing-structure-activity”relationships of polysaccharides in Chinese Materia Medica:a comprehensive review[J].Carbohydrate Polymers,2025,358:123503.
12. [12]
  Chen L,Xin X L,Li S G,et al.Optimization of polysaccharides and essential oils extraction from Artemisia argyi leaves and the antioxidant activity of polysaccharides[J].Journal of Biobased Materials and Bioenergy,2023,17(5):631-640.
13. [13]
  徐百昌,谢宗固,司红彬.构树多糖的提取工艺、结构表征和生物活性研究进展[J].中国畜牧兽医,2025,52(6):2662-2674.
  Xu B S,Xie Z G,Si H S,et al.Research progress on extraction techniques,structural characterization and biological activities of polysaccharides derived from Broussonetia papyrifera[J].China Animal Husbandry and Veterinary Medicine,2025,52(6):2662-2674.
14. [14]
  Li N N,Fu X D,Xiao M S,et al.Enzymatic preparation of a low-molecular-weight polysaccharide rich in uronic acid from the seaweed Laminaria japonica and evaluation of its hypolipidemic effect in mice[J].Food & Function,2020,11(3):2395-2405.
15. [15]
  Hu W J,Yu A Q,Bi H Z,et al.Recent advances in Artemisia argyi Levl.et Vant.polysaccharides:extractions,purifications,structural characteristics,pharmacological activities,and existing and potential applications[J].International Journal of Biological Macromolecules,2024,279:135250.
16. [16]
  Ruan Y,Niu C F,Zhang P Z,et al.Acid-catalyzed water extraction of two polysaccharides from Artemisia argyi and their physicochemical properties and antioxidant activities[J].Gels,2022,8(1):5.
17. [17]
  万丹娜.考马斯亮蓝法测定奶粉中蛋白质含量的研究[J].中国食品工业,2024,(17):88-90. Wan D N.Study on the determination of protein content in milk powder by the Coomassie blue method [J].China Food Industry, 2024
  ,(17):88-90.
18. [18]
  马盖凡,姜雪莲,侯明槠,等.艾叶多糖提取、分离纯化及生物活性研究进展[J].中国医院药学杂志,2022,42(11):1164-1169.
  Ma G F,Jiang X L,Hou M Z,et al.Research progress in the extraction,separation,purification and biological activities of polysaccharides from leaves of Artemisia argyi[J].China Journal of Hospital Pharmacy,2022,42(11):1164-1169.
19. [19]
  楚三慧,张俊俊,杨冉,等.艾叶多糖提取方法及其药理作用研究进展[J].海峡药学,2021,33(11):41-44.
  Chu S H,Zhang J J,Yang R,et al.Research progress on extraction methods and pharmacological effects of polysaccharide from Artemisia argyi[J].Strait Pharmacy,2021,33(11):41-44.
20. [20]
  周睿,吴江萍,姜娜娜,等.绿茶末多糖的理化特性及其体外抗氧化和抗糖尿病活性[J].食品工业科技,2023,44(1):1-13.
  Zhou R,Wu J P,Jiang N N,et al.Physicochemical properties and in vitro antioxidant and antidiabetic activities of green tea dust polysaccharide[J].Food Industry Science and Technology,2023,44(1):1-13.
21. [21]
  李欣琪,张鹰,白卫东,等.藏天麻多糖提取工艺优化及其基本组成分析[J].粮食与油脂,2025,38(8):98-103.
  Li X Q,Zhang Y,Bai W D,et al.Optimization of extraction process of polysaccharides from Tibetan Gastrodia elata and analysis of its basic composition[J].Grain and Oils,2025,38(8):98-103.
22. [22]
  胡木,欧兰梅,肖益东.山药多糖的提取、纯化及结构分析[J].食品工业,2025,46(8):48-52.
  Hu M,Ou L M,Xiao Y D,et al.Extraction,purification and structural analysis of polysaccharides from Tiegun Yam[J].Food Industry,2025,46(8):48-52.
23. [23]
  Wang Z C,Zheng Y,Lai Z R,et al.Effect of monosaccharide composition and proportion on the bioactivity of polysaccharides:a review[J].International Journal of Biological Macromolecules,2024,254:127955.
24. [24]
  Ning E J,Sun C W,Wang X F,et al.Artemisia argyi polysaccharide alleviates intestinal inflammation and intestinal flora dysbiosis in lipopolysaccharide-treated mice[J].Food & Medicine Homology,2024,1(1):9420008.
25. [25]
  Yu J,Cao Y,He F,et al.Polysaccharides from Artemisia argyi leaves:environmentally friendly ultrasound-assisted extraction and antifatigue activities[J].Ultrasonics Sonochemistry,2024,107:106932.
26. [26]
  Wang Z C,Zhou X Y,Shu Z H,et al.Regulation strategy,bioactivity,and physical property of plant and microbial polysaccharides based on molecular weight[J].International Journal of Biological Macromolecules,2023,244:125360.
27. [27]
  Wang Z C,Zhou X Y,Sheng L L,et al.Effect of ultrasonic degradation on the structural feature,physicochemical property and bioactivity of plant and microbial polysaccharides:a review[J].International Journal of Biological Macromolecules,2023,236:123924.
28. [28]
  Yang Y,Lai Z,Hu X,et al.Structural characterization and combined immunomodulatory activity of fermented Chinese yam polysaccharides with probiotics[J].International Journal of Biological Macromolecules,2025,307:142290.
29. [29]
  Wang Z C,Zheng Y,Lai Z R,et al.Effect of Saccharomyces cerevisiae CICC 32883 fermentation on the structural features and antioxidant protection effect of Chinese yam polysaccharide[J].Foods,2025,14(4):564.
30. [30]
  Wang S Y,Chang H,Li N,et al.Structural characterization and immunomodulatory activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882[J].Scientific Reports,2025,15(1):19992.
31. [31]
  Wang S Y,Li N,Zhang Y,et al.Immunoregulatory activity of an alkaline-precipitated polysaccharide CGP-AP produced by Chaetomium globosum CGMCC 6882[J].Carbohydrate Polymer Technologies and Applications,2024,7:100430.
32. [32]
  Wang Z C,Zhou X Y,Liang X N,et al.Antioxidant and antibacterial activities of a polysaccharide produced by Chaetomium globosum CGMCC 6882[J].International Journal of Biological Macromolecules,2023,233:123628.
33. [33]
  Abubakar A S,Ahmad B,Ahmad N,et al.Physicochemical evaluation,structural characterization,in vitro and in vivo bioactivities of water-soluble polysaccharides from Luobuma (Apocynum L.)tea[J].Food Chemistry,2024,460:140453.
34. [34]
  Zeng S Y,Wang B,Lv(LYU) W Q,et al.Physicochemical properties,structure and biological activity of ginger polysaccharide:effect of microwave infrared dual-field coupled drying[J].International Journal of Biological Macromolecules,2024,281:136474.
35. [35]
  Wu J S,Wu Z Y,Pan Y R,et al.Effects of different stress conditions on the production,bioactivities,physicochemical and structural characteristics of exopolysaccharides synthesized by Schleiferilactobacillus harbinensis Z171[J].International Journal of Biological Macromolecules,2024,257:128675.
36. [36]
  Ma G F,Zhao B B,Tang P L,et al.Structural characterization and immunomodulatory activity of polysaccharides from the roots of Artemisia argyi[J].Carbohydrate Polymers,2025,368:124169.
37. [37]
  Ni S,Zhao H L,Yang S H,et al.The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion[J].Ultrasonics Sonochemistry,2025,116:107329.
38. [38]
  Wang Z C,Zheng Y,Dai Y D,et al.Effect of probiotic fermentation on the extraction rate and bioactivity of plant-based polysaccharides:a review[J].Innovative Food Science & Emerging Technologies,2024,98:103863.
39. [39]
  Wang Z C,Zheng Y,Zhou X Y,et al.Effect of Lactobacillus fermentation on the structural feature,physicochemical property,and bioactivity of plant and fungal polysaccharides: a review[J].Trends in Food Science & Technology,2024,148:104492.
40. [40]
  Imai T,Takahashi Y.Chemotaxis assay for Marsupenaeus japonicus hemocytes and application for the development of an oral immunostimulant against white spot syndrome virus[J].Frontiers in Cell and Developmental Biology,2020,8:613.
41. [41]
  庞心悦.祁白芷多糖的结构表征及对巨噬细胞氧化损伤的保护作用研究[D].天津:天津科技大学,2023. Pang X Y.Structural characterization of polysaccharides from Angelicae Dahuricae Radix and their protective effects against oxidative damage in macrophages[D].Tianjin:Tianjin University of Science and Technology,2023.
42. [42]
  Zhou H J,Fan Z,Li Y,et al.Structure-antioxidant activity relationship of polysaccharides isolated by microwave/ultrasonic-assisted extraction from Pleurotus ferulae[J].Antioxidants,2025,14(1):91.
1. [1]
  郑巧文 , 孙怡宁 , 邓玮亮 , 陈小乐 , 陈坤锥 , 覃威铭 , 申迎宾 , 张磊 . 喀什“新新2号”核桃油组成特征及其在HepG2细胞中调节脂质代谢与抗氧化活性评价. 轻工学报, 2026, 41(3): 28-40. doi: 10.12187/2026.03.004
2. [2]
  徐愉聪 , 李雯政 , 范茂梅 , 步营 , 朱文慧 , 励建荣 , 李学鹏 . 基于密度泛函理论的EGCG与茶黄素抗氧化活性对比研究. 轻工学报, 2025, 40(6): 43-53. doi: 10.12187/2025.06.005
3. [3]
  张馨月 , 闫倩楠 , 杨泽豪 , 于淼 , 马挺军 . 虾青素鸡蛋的营养活性及风味研究. 轻工学报, 2025, 40(1): 41-48. doi: 10.12187/2025.01.005
4. [4]
  吕强 , 韩小贤 , 李力 , 马森 . 羧甲基化改性对麦麸膳食纤维特性的影响. 轻工学报, 2026, 41(3): 62-75. doi: 10.12187/2026.03.007
5. [5]
  郭水欢 , 袁思洁 , 郭楠楠 , 陈贤 , 樊亚敏 , 高晗 , 詹丽娟 . 发光二极管调控芽苗菜品质的研究进展. 轻工学报, 2025, 40(1): 49-57. doi: 10.12187/2025.01.006
6. [6]
  禹晓 , 李升 , 张晨 , 杨帆 , 李楚瑜 , 陈亚淑 , 邓乾春 , 朱莹莹 . 市售亚麻籽粉营养品质分析. 轻工学报, 2026, 0(0): -.
7. [7]
  李琪琳 , 刘菲 , 耿在军 , 何姣 , 王倩 , 李丽 , 杨素珍 . 蛹虫草双向发酵组合物的抗氧化、抗皱紧致及光老化修复功效评价. 轻工学报, 2026, 41(4): 134-142. doi: 10.12187/2026.04.013
8. [8]
  左丽超 , 宝媛媛 , 曲召辉 , 吴珂 , 李姝静 , 李晓珍 , 孟宏 , 何一凡 . 氧化铈抗氧化及抗光老化性能研究. 轻工学报, 2025, 40(6): 118-126. doi: 10.12187/2025.06.012
9. [9]
  雷露 , 许浩翔 , 李婷 , 周景瑞 , 齐婧 , 艾蓉 , 罗文菊 , 姜玲玲 . 黄花梨蜂蜜与其他蜜源蜂蜜的抗氧化特性研究. 轻工学报, 2025, 40(1): 32-40. doi: 10.12187/2025.01.004
10. [10]
  雷月馨 , 程张晨 , 陈瑾 , 贺禹丰 , 肖香 . 不同大麦多酚的抗氧化及降脂能力差异研究. 轻工学报, 2025, 0(0): -.
11. [11]
  雷月馨 , 程张晨 , 陈瑾 , 贺禹丰 , 肖香 . 不同大麦多酚的抗氧化及降脂能力差异研究. 轻工学报, 2025, 40(4): 30-40. doi: 10.12187/2025.04.004
12. [12]
  倪众 , 楚鹏飞 , 林颖 , 刘玉欣 . 低温长时间热处理过程中氧化和加热对海参体壁胶原纤维结构的影响. 轻工学报, 2025, 40(1): 21-31,57. doi: 10.12187/2025.01.003
13. [13]
  李雅欣 , 罗敏 , 刘元福 , 孙全娇 , 黄俊铭 , 文建霞 , 杜文雅 , 张良 . 低共熔溶剂提取川明参多糖工艺优化与结构表征. 轻工学报, 2026, 41(1): 47-58. doi: 10.12187/2026.01.005
14. [14]
  蒋纬 , 胡颖 , 朱振元 . 阳荷多糖提取工艺优化及其生物活性研究. 轻工学报, 2025, 40(4): 10-19. doi: 10.12187/2025.04.002
15. [15]
  郭向阳 , 王璐璐 , 马景可 , 金珂婷 . 超声波降解多糖的作用表现、影响因素及机理研究进展. 轻工学报, 2025, 40(4): 41-51. doi: 10.12187/2025.04.005
16. [16]
  甘晓锟 , 陈坤锥 , 申迎宾 , 张磊 , 李湛 . 鹿茸菇多糖对免疫抑制小鼠免疫及肠道菌群的作用. 轻工学报, 2025, 40(6): 54-66. doi: 10.12187/2025.06.006
17. [17]
  王尧 , 颜锐坤 , 梁雪梅 , 叶利经 , 吴帅凯 , 钟琼 , 刘莉莉 , 赵国军 . 植物多糖缓解结肠炎的作用机制与构效关系研究进展. 轻工学报, 2026, 41(5): 25-36. doi: 10.12187/2026.05.003
18. [18]
  李良玉 , 张思影 , 李雪静 , 曹荣安 , 王坤 , 王菲菲 . 毛蜂窝菌粗多糖超声波辅助提取工艺优化及其体外生物活性研究. 轻工学报, 2026, 41(5): 1-12. doi: 10.12187/2026.05.001
19. [19]
  车静 , 李昕昕 , 李赞 , 韩磊 , 雷珊珊 , 江军刚 , 张一帆 , 王磊 . 基于TEMPO氧化纤维的功能性滤棒制备及其应用研究. 轻工学报, 2025, 40(5): 118-126. doi: 10.12187/2025.05.014
20. [20]
  车静 , 李昕昕 , 李赞 , 韩磊 , 雷珊珊 , 江军刚 , 张一帆 , 王磊 . 基于TEMPO氧化纤维的功能性滤棒的制备及其应用研究. 轻工学报, 2025, 0(0): -.

点击查看大图

计量

PDF下载量: 2
文章访问数: 250
引证文献数: 0

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

艾草多糖结构特征及抗氧化活性研究

作者简介: 王子朝(1985—),男,河南省林州市人,河南工业大学副教授,博士,主要研究方向为糖化学与糖生物学。E-mail:6832917@163.com;

Structural characterization and antioxidant activity of Artemisia argyi polysaccharids

计量

通讯作者: 陈斌, bchen63@163.com

艾草多糖结构特征及抗氧化活性研究

作者简介:王子朝(1985—),男,河南省林州市人,河南工业大学副教授,博士,主要研究方向为糖化学与糖生物学。E-mail:6832917@163.com

English Abstract

Structural characterization and antioxidant activity of Artemisia argyi polysaccharids

目录

艾草多糖结构特征及抗氧化活性研究

作者简介: 王子朝(1985—),男,河南省林州市人,河南工业大学副教授,博士,主要研究方向为糖化学与糖生物学。E-mail:6832917@163.com;

Structural characterization and antioxidant activity of Artemisia argyi polysaccharids

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

艾草多糖结构特征及抗氧化活性研究

作者简介:王子朝(1985—),男,河南省林州市人,河南工业大学副教授,博士,主要研究方向为糖化学与糖生物学。E-mail:6832917@163.com

English Abstract

Structural characterization and antioxidant activity of Artemisia argyi polysaccharids

目录