双菌株联合发酵提高鹰嘴豆纳豆激酶活力的研究
Study of improving chickpeanattokinase activity through fermentation by two compound strains
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摘要: 以鹰嘴豆为原料,选取两株不同来源的纳豆芽孢杆菌F-2-4和S-15,分别通过单接种和混合接种的方式,制备鹰嘴豆纳豆,并测定其纳豆激酶活力,结果表明:通过混合接种方式制备的鹰嘴豆纳豆的纳豆激酶活力明显高于单接种方式,当F-2-4与S-15接种体积比为1:1,且接种量为4%时,鹰嘴豆纳豆的纳豆激酶活力达到最高值6 287.16 UI/g.Abstract: With chickpea as material,two Bacillus subtilisnatto strains (F-2-4 and S-15) isolated from different sources were selected and were inoculated into chickpea to prepare chickpea natto products by single-inoculation and co-inoculation,then nattokinase activity of chickpea natto was determined.Results showed that nattokinase activity was improved significantly by co-inoculation.When the inoculative volume ratio of F-2-4 and S-15 was 1:1,and inoculation amount was 4%,the nattokinase activity of chickpea natto reached the maximum 6 287.16 UI/g.
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Key words:
- two strains fermenta-tion /
- chickpea /
- Bacillus subtilisnatto /
- nattokinase
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