绿豆蛋白提取工艺的响应面法优化
Optimization of protein extraction from mung bean using response surface methodology
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摘要: 以绿豆为原料,采用碱提酸沉法提取绿豆蛋白,通过单因素试验研究绿豆粉与蒸馏水质量比、提取温度、提取时间和pH值对绿豆蛋白提取率的影响,并通过响应面法优化绿豆蛋白的提取工艺.结果表明:绿豆蛋白的最佳提取工艺条件为绿豆粉与蒸馏水质量比1:15,提取温度40℃,提取时间20 min,pH值9.0,该条件下绿豆蛋白的最大提取率可达88.80%.Abstract: With mung bean as raw material,alkaline-extraction method was used to obtain mung bean was made by proteins.The effect of mung bean flour and distilled water mass ratio, extraction temperature, extraction time and pH value on the mung bean protein extraction rate were studied by single factor experiment.Optimization of protein extraction from mung bean was made by using response surface methodology.The results showed that the optimum extraction conditions for mung bean protein were the ratio between mung bean flour and distilled water mass 1:15, extraction temperature 40℃, extraction time 20 min and pH value 9.0.Under these conditions, the maximal extraction rate of mung bean protein was 88.80%.
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[1]
程须珍,王素华,田静,等.绿豆优异种质综合评价[J].中国农业科学, 1999,32:36.
-
[2]
XIE J H,DU M X,SHEN M Y,et al.Physico-chemical properties, antioxidant activities and angiotensin-I converting enzyme inhibitory of protein hydrolysates from mung bean (Vigna radiate)[J].Food Chemistry,2019(270):243.
-
[3]
DU M,XIE J,GONG B,et al.Extraction,physicochemical characteristics and functional properties of mung bean protein[J].Food Hydrocolloids, 2018,76:131.
-
[4]
WANG Z,HAN F,SUI X,et al.Effect of ultrasound treatment on the wet heating Maillard reaction between mung bean (Vigna radiate (L.)) protein isolates and glucose and on structural and physico-chemical properties of conjugates[J].Journal of the Science of Food and Agriculture, 2016,96(5):1532.
-
[5]
KHAKET T P,DHANDA S,JODHA D.Purification and biochemical characterization of dipeptidyl peptidase-Ⅱ (DPP7) homologue from germinated Vigna radiata seeds[J].Bioorganic Chemistry,2015,63:132.
-
[6]
于鹏亮.茶渣中蛋白质和多糖的综合提取及其分离纯化[D].合肥:安徽农业大学,2013.
-
[7]
史卿.富硒菜籽粕蛋白功能性质及抗氧化活性的研究[D].南昌:南昌大学,2013.
-
[8]
程水明,赵俊仁,王春,等.木瓜蛋白酶酶解海蜇脑蛋白工艺的优化[J].湖北农业科学,2014,53(7):1649.
-
[9]
CHENG S M, ZHAO J R,LIU J F, et al. Technology for enzymolysis of jellyfish brain protein by bromelain[J].Agricultural Science & Technology, 2013, 14(10):1486.
-
[10]
张颖,张光艳,王宇翔,等.不同花源蜂蜜蛋白质组分及提取方法的比较[J].食品与发酵工业,2019,45(14):91.
-
[11]
顾文芬,曾凡逵,程锦春.超滤法从马铃薯淀粉加工分离汁水中回收蛋白质的研究[J].现代食品科技,2018,34(3):131.
-
[12]
江连洲,张巧智,李杨,等.大豆水酶法水解液中蛋白质超滤回收及特性研究[J].农业机械学报,2017,48(2):327.
-
[13]
赵斌,杨鑫,洪剑鹏,等.超滤过程中蛋白质的空间分布规律[J].天津工业大学学报,2019,38(3):22.
-
[14]
李国军,姜媛媛,王莹,等.超滤技术在蛋白质分离纯化中应用[J].生物技术世界,2016(3):227.
-
[15]
AVAN U D,MCKENZIE D B,SHAHIDI F.Functional properties of protein isolates from beach pea (Lathyrus maritimus L.)[J].Food Chemistry, 2001, 74(2):177.
-
[16]
THOMPSON L U.Preparation and evaluation of mung bean protein isolates[J].Journal of Food Science,1977,42:1365.
-
[17]
ADSUALE R N.Chemistry and technology of green grain,CRC critical review[J].Food Science and Nutrition,1986,25(1):73.
-
[18]
刘咏,杨柳.绿豆蛋白质提取工艺的优化[J].食品科学,2008,29(8):272.
-
[19]
陈翔宇,邹怡然,蒋天宁,等.响应面法优化碱溶酸沉法提取黄秋葵蛋白工艺条件研究[J].海南师范大学学报(自然科学版),2019,32(3):288.
-
[20]
王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009,23(3):40.
-
[21]
中国国家标准化委员会.食品中蛋白质的测定:GB 5009.5-2016[S].北京:中国标准出版社,2016.
-
[22]
KIM E O, LEE J Y, CHOI S W.Quantitative changes in phenolic compounds of safflower (Carthamus tinctorius L.) seeds during growth and processing[J].Preventive Nutrition and Food Science, 2006, 11(4):311.
-
[23]
李永武.绿豆清蛋白的提取及功能特性和理化性质的研究[D].大庆:黑龙江八一农垦大学,2014.
-
[24]
LAWAL O S.Functionality of African locust bean (Parkia biglobossa) protein isolate:Effects of pH,ionic strength and various protein concentrations[J].Food Chemistry,2004,86:345.
-
[25]
SHIH F F,DAIGLE K.Use of enzymes for the separation of protein from rice flour[J].Cereal Chemistry,1997,74(4):437.
-
[26]
CAI W,GU X,TANG J.Extraction,purification,and characterization of the polysaccharides from Opuntia milpa alta[J].Carbohydrate Polymers,2008,71(3):403.
-
[27]
李静娟,易建华,朱振宝.响应面法优化桃仁蛋白提取工艺[J].中国油脂,2010(35):20.
-
[28]
陈建旭,黄生权,敖宏,等.赤灵芝中水溶性蛋白响应面法优化提取[J].现代食品科技,2009,25(6):661.
-
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