JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 35 Issue 2
April 2020
Article Contents
WEI Tao, CHEN Shunxin, HUANG Shen, et al. Optimization of fermentation conditions of β-carotenes degradating strain HC-3 and its effect on aroma enhancement of reconstituted tobacco concentrate[J]. Journal of Light Industry, 2020, 35(2): 24-32. doi: 10.12187/2020.02.004
Citation: WEI Tao, CHEN Shunxin, HUANG Shen, et al. Optimization of fermentation conditions of β-carotenes degradating strain HC-3 and its effect on aroma enhancement of reconstituted tobacco concentrate[J]. Journal of Light Industry, 2020, 35(2): 24-32. doi: 10.12187/2020.02.004 shu

Optimization of fermentation conditions of β-carotenes degradating strain HC-3 and its effect on aroma enhancement of reconstituted tobacco concentrate

  • Received Date: 2019-10-24
  • The fermentation conditions of β-carotene degrading strain HC-3 were optimized by single factor experiment and orthogonal experiment,and the fermentation liquid of strain HC-3 obtained under the optimal fermentation conditions was applied to the reconstituted tobacco concentrate to study its aroma enhancing effect.The results showed that the best fermentation conditions for β-carotene degradation were 3 g/L of NaNO3,30 g/L of sucrose,3 g/L of yeast powder and 7.0 of initial pH value.Under this condition,the degradation rate of β-carotene could reach 93.35%.After being treated by strain HC-3,the eight flavor components of dihydroactyl lactone, 4,7,9-Mega three ene-3- keton, 4- hydroxyl-β-two hydrogen damascene,9-hydroxy-4,7-Mega three ene ketene,triethyl citrate, 4- (3-hydroxyl butyl)-3,5,5- three methyl-2- cyclohexene-1- ketone,tetradecanoic acid and neophytadiene in the reconstituted tobacco concentrate were significantly increased.Compared with the untreated reconstituted tobacco concentrate,the fragrances made from the reconstituted tobacco concentrate treated by strain HC-3 had better quality,more aroma,higher aroma concentration and lower offensive odor content.
  • 加载中
    1. [1]

      李爱军,代慧娟,娄本,等.烟草类胡萝卜素研究进展[J].安徽农业科学,2008,36(6):2364.

    2. [2]

      EROGLU A,HRUSZKEWYCZ D P,CURLEY-JR R W,et al.The eccentric cleavage product of β-carotene,β-apo-13-carotenone,functions as an antagonist of RXRa[J].Archives of Biochemistry and Biophysics.2010,504(1):11.

    3. [3]

      CHU D Y,XIE Y Q,HONG S Q,et al.Major carotenoids in tobacco laminas:Identification and quantification by HPLC with photodiode array detection[J].Asian Journal of Chemistry,2010,22(4):2635.

    4. [4]

      LEFFINGWELL J C.Carotenoids as flavour & Fragrance precursors[J].Leffingwell Reports,2002,2(3):1.

    5. [5]

      李福枝,刘飞,曾晓希,等.天然类胡萝卜素的研究进展[J].食品工业科技,2007,28(9):227.

    6. [6]

      王瑞新.烟草化学[M].北京:中国农业出版社,2003.

    7. [7]

      HARRISON P J,BUGG T D.Enzymology of the carotenoid cleavage dioxygenases:Reaction mechanisms,inhibition and biochemical roles[J].Archives of Biochemistry and Biophysics,2014,544(4):105.

    8. [8]

      SILVA I,ROCHA S M,COIMBRA M A.Quantification and potential aroma contribution of β-ionone in marine salt[J].Flavour and Fragrance Journal,2010,25(4):93.

    9. [9]

      SCHWARTZ S H,QIN X Q,ZEEVAART J A D.Characterization of a novel carotenoid cleavage dioxygenase from plants[J].The Journal of Biological Chemistry,2001,276(27):25208.

    10. [10]

      MARASCO1 E K,VAY K,DANNERT S C.Identification of carotenoid cleavage dioxygenases from Nostoc sp.PCC 7120 with different cleavage activities[J].The Journal of Biological Chemistry,2006,281(42):31583.

    11. [11]

      NONIER M F,GAULEJAC N V D,VIVAS N,et al.Characterization of carotenoids and degradation products in oak wood incidence on the flavour of wood[J].Comptes Rendus Chimie,2004,7(6/7):689.

    12. [12]

      刘金霞.烤烟类胡萝卜素的提取分离、氧化降解与加香应用研究[D].郑州:河南农业大学,2008.

    13. [13]

      KANEKO H,HARADA M.4-Hydroxy-β-damascone and 4-Hydroxy-dihydro-β-damascone from Cigar tobacco[J].Agricultural Biology Chemistry,1972,36(1):168.

    14. [14]

      张溪,刘皓月,惠岚峰,等.造纸法再造烟叶的研究进展[J].天津造纸,2018,40(1):2.

    15. [15]

      戴路,卢昕博,吴志宏,等.加工工艺对再造烟叶感官品质的影响[J].食品工业,2017,38(10):31.

    16. [16]

      孙先玉,孙博,李冬玲,等.造纸法再造烟叶加工技术研究进展[J].生物质化学工程,2011,45(6):49.

    17. [17]

      魏涛,杨坤鹏,黄申,等.降解β-胡萝卜素产香菌株的分离鉴定及降解酶酶学性质[J].中国食品学报,2018,18(4):94.

    18. [18]

      蔡冰,王建新,陈祖刚,等.造纸法再造烟叶致香成分的分析[J].烟草科技,2002(6):19.

    19. [19]

      贾蓓蕾,魏涛,黄申,等.α-胡萝卜素降解产香菌株的分离﹑鉴定及发酵条件优化[J].食品与发酵工业,2015,41(1):34.

    20. [20]

      邱晔,王建,马讯,等.造纸法再造烟叶感官评价方法的研究[J].湖北农业科学,2014,53(18):4351.

Article Metrics

Article views(2449) PDF downloads(30) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return