JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 35 Issue 3
May 2020
Article Contents
ZHANG Junjie, GUO Chen, PENG Shanshan, et al. Analysis of genetic diversity of 7 tea plant varieties from Fujian and construction of their molecular fingerprints[J]. Journal of Light Industry, 2020, 35(3): 19-27. doi: 10.12187/2020.03.003
Citation: ZHANG Junjie, GUO Chen, PENG Shanshan, et al. Analysis of genetic diversity of 7 tea plant varieties from Fujian and construction of their molecular fingerprints[J]. Journal of Light Industry, 2020, 35(3): 19-27. doi: 10.12187/2020.03.003 shu

Analysis of genetic diversity of 7 tea plant varieties from Fujian and construction of their molecular fingerprints

  • Received Date: 2020-02-07
  • Based on the SSR molecular marking technology, it analyzed the level of genetic diversity of 7 tested tea plant varieties from Fujian province and constructed the molecular fingerprint of them. The results showed that all of the 14 molecular markers primary screening were polymorphic markers with rich polymorphism information, and 7 tested tea plant varieties could be distinguished effectively. Genetic similarity coefficient between Fuyun No.6 and Fuyun No.7 was highest(8.06) which had a close phylogenetic relationship. 3 SSR molecular markers(PS3, PS15, PS19) were selected to construct fingerprint because of less amplified bands and higher Polymorphism, and the fingerprint could distinguish 7 tea plant varieties, achieved the purpose of identifying all varieties with the least molecular marker.
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    1. [1]

      KAMUNYA S M,WACHIRA F N,PATHAK R S,et al.Genomic mapping and testing for quantitative trait loci in tea (Camellia sinensis(L.) O. Kuntze)[J]. Tree Genetics and Genomes, 2010,6(6):915.

    2. [2]

      黄丹娟.我国优良茶树品种遗传多样性分析及指纹图谱构建[D].北京:中国农业科学院,2016.

    3. [3]

      CHEN L,ZHOU Z X,YANG Y J.Genetic improvement and breeding of tea plant (Camellia sinensis) in China:From individual selection to hybridization and molecular breeding[J].Euphytica,2007,154(1/2):239.

    4. [4]

      陈亮,虞富莲,姚明哲,等.国际植物新品种保护联盟茶树新品种特异性、一致性、稳定性测试指南的制订[J].中国农业科学,2008,41(8):2400.

    5. [5]

      魏倩倩.分子标记技术在家禽育种中的应用[J].农民致富之友,2017(17):71.

    6. [6]

      李冰敏.分子标记在园林植物育种中的应用[J].现代园艺,2019(15):148.

    7. [7]

      冯英娜.茄子遗传多样性与主要农艺性状标记关联分析[D].南京:南京农业大学,2014.

    8. [8]

      鲁亚静,陈庭,李建友,等.叶子花SSR-PCR体系的优化[J].安徽农业科学, 2015(17):57.

    9. [9]

      DETTORI M T,MICALI S,GIOVINAZZI J,et al.Mining microsatellites in the peach genome:Development of new long-core SSR marker for genetic analyses in five Prunus species[J].Springerplus,2015,4(1):337.

    10. [10]

      刘本英,孙雪梅,李友勇,等.基于EST-SSR标记的云南无性系茶树良种遗传多样性分析及指纹图谱构建[J].茶叶科学,2012(3):77.

    11. [11]

      余继忠,黄海涛,姚明哲,等.基于EST-SSR的福云(半)同胞系茶树品种(系)遗传多样性和亲缘关系分析[J].茶叶科学,2010(3):32.

    12. [12]

      章志芳,马建强.基于SSR标记的茶树新品种遗传多样性分析及指纹图谱构建[J].湖南农业科学,2012(19):9.

    13. [13]

      孟清照,李仕金,董转年,等.SSR引物开发方法概述[J].大众科技,2007(6):116.

    14. [14]

      杨阳,刘振,赵洋,等.湖南省主要茶树品种分子指纹图谱的构建[J].茶叶科学,2010,30(5):367.

    15. [15]

      朱岩芳.作物品种分子标记鉴定及指纹图谱构建研究[D].杭州:浙江大学,2013.

    16. [16]

      徐丽芳,陈吉炎,罗光明.分子标记技术及其在植物育种中的应用[J].食品与药品,2007,9(10):43.

    17. [17]

      刘洪伟.茶树gSSR分子指纹开发及其在商品茶品种鉴别中的应用[D].合肥:安徽农业大学,2016.

    18. [18]

      郭燕,刘声传,曹雨,等.基于SSR标记贵州古茶树资源的遗传多样性分析及指纹图谱构建[J].西南农业学报, 2016,29(3):491.

    19. [19]

      金基强,崔海瑞,龚晓春,等.用EST-SSR标记对茶树种质资源的研究[J].遗传,2007,29(1):103.

    20. [20]

      乔婷婷,马春雷,周炎花,等.浙江省茶树地方品种与选育品种遗传多样性和群体结构的EST-SSR分析[J].作物学报,2010,36(5):744.

    21. [21]

      陈志辉,单睿阳,游小妹,等.43个福建省茶树品种指纹图谱构建及遗传多样性分析[J].热带植物学报,2017(6):579.

    22. [22]

      王让剑,杨军,孔祥瑞,等.福建15个茶树品种SSR遗传差异分析与指纹图谱建立[J].福建农业学报,2014(10):970.

    23. [23]

      DOYLE J J.DNA protocols for plants[J].Molecular Techniques in Taxonomy, 1991(57):283.

    24. [24]

      UJIHARA T,OHTA R,HAYASHI N,et al.identification of Japanese and Chinese green tea cultivars by using simple sequence repeat markers to encourage proper labeling[J].Bioscience Biotechnology and Biochemistry,2009,73(1):15.

    25. [25]

      吴晓梅,姚明哲,马春雷,等.利用EST-SSR标记研究适制绿茶与乌龙茶品种的遗传多样性与遗传结构[J].茶叶科学,2010, 30(3):195.

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