嗜麦芽窄食单胞菌的培养基优化及其在烟叶发酵中的初步应用研究
Optimization of medium for Stenotrophomonas sp.and its preliminary application in tobacco fermentation
-
摘要: 采用单因素试验对嗜麦芽窄食单胞菌（Stenotrophomonas sp.H3-1）的培养基进行优化，在此基础上，将该菌株制备成菌制剂后初步应用于烟叶发酵，并对比研究发酵前后烟叶的品质变化.结果表明：优化后嗜麦芽窄食单胞菌培养基中适宜的碳源为质量浓度为2.0 g/L的麦芽糖，氮源为质量浓度为2.0 g/L的硫酸铵，初始pH值为8，培养温度为40℃；烟叶发酵后有5种香味物质的质量浓度发生了明显变化，其中对香气组成影响较大的茄酮和9-羟基-4，7-巨豆二烯-3-酮的质量浓度均有不同程度的增加；评吸结果表明，利用菌制剂发酵烟叶后，其香气质、香气量和香气浓度均有所提升.Abstract: The medium of Stenotrophomonas sp.H3-1 was optimized by one single factor experiment.The strain was prepared into bacteria-preparation and preliminarily used in tobacco fermentation,and the quality changes of tobacco leaves before and after fermentation were compared.It showed that the optimized culture medium was 2.0 g/L of maltose asthe carbon source and the nitrogen source is ammonium sulfate of 2.0 g/L,initial pH value 8,temperature 40℃.After tobacco fermentation,the mass concentrations of five flavor substances changed obviously,the mass concentrations of solanone and 9-hydroxy-4,7-megadiene-3-one,which had greater effect on aroma composition,all increased to different degrees.The evaluation results showed that the aroma quality,aroma quantity and aroma concentration of tobacco leaves were improved after fermented with bacterial preparations.
-
-
[1]
张思琦,何佳,周方,等.不同烤烟品种(系)二萜类物质合成关键基因的表达及代谢差异[J].西北农林科技大学学报(自然科学版),2019,47(9):25.
-
[2]
杨鹏飞,华涛,黄申,等.烟草中西松烷型二萜类化学成分研究进展[J].化学试剂,2020,42(4):380.
-
[3]
王冬,张小全,彭玉富,等.昼夜温差和光照强度对烟草西柏三烯二醇的合成及CYC-1、CYP71D16基因表达的影响[J].华北农学报,2014(5):150.
-
[4]
李国强,张艳玲,林文翰.西松烷二萜类海洋活性成分研究进展[J].中国海洋大学学报,2006,36(3):370.
-
[5]
CRUICKSHANK I,PERRIN D R,MANDRYK M.Fungitoxicity of duvatrienediols associated with the cuticular wax of tobacco leaves[J].Journal of Phytopathology,2010,90(3):243.
-
[6]
闫克玉.烟草化学[M].郑州:郑州大学出版社,2002:1-10.
-
[7]
CHIANG S Y,GRUNWALD C.Duvatrienediol,alkanes,and fatty acids in cuticular wax of tobacco leaves of various physiological maturity[J].Phytochemistry,1976,15(6):961.
-
[8]
毛多斌,张槐岭,贾春晓.卷烟香味化学[M].郑州:河南科技出版社,1994:343-345.
-
[9]
黄志,毕艳玖,沙云菲,等.在线液相色谱-气相色谱/质谱联用快速分析烟叶中的西柏三烯二醇[J].上海大学学报(自然科学版),2020,26(2):227.
-
[10]
GRIFFITH R B,JOHUSON R R,QUINN A D.Organoleptically imporved tobacco product:US3174485A[P].1965-03-23.
-
[11]
王冬,张小全,杨铁钊,等.类西柏烷二萜代谢机理及调控研究进展[J].中国烟草学报,2014,20(3):113.
-
[12]
JACKSON D M,SEVERSON R F,JOHNSON A W,et al.Ovipositional response of tobacco budworm moths(Lepidoptera:Noctuidae) to cuticular chemical isolates from green tobacco leaves.[J].Environmental Entomology,1984,13(4):1023.
-
[13]
SAYED K E,LAPHOOKHIEO S,YOUSAF M,et al.Semisynthetic and biotransformation studies of (1S,2E,4S,6R,7E,11E)-2,7,11-cembratriene-4,6-diol[J].Journal of Natural Products,2008,71(1):117.
-
[14]
ARNARP J,ALEXIS C W L,ENZELL C R,et al.ChemInform abstract:tobacco chemistry.Part 76.Biotransformations of tobacco isoprenoids using plant cell cultures of Tripterygium wilfordii[J].ChemInform,2010,24(42):683.
-
[15]
ARNARP J,CHU W,ENZELL C R,et al.ChemInform abstract:tobacco chemistry.Part 77.Biotransformations of a major tobacco cembratrienediol using plant cell cultures of Nicotiana sylvestris[J].Acta Chem Scand,1993,47:79.
-
[16]
GUO Z,WAGNER G J.Biosynthesis of cembratrienols in cell-free extracts from trichomes of Nicotianatabacum[J].Plant Science,1995,110(1):1.
-
[17]
RALDUGIN V A,SALENKO V L,YAROSHENKO N I,et al. Oxidative transformations of cembrane diterpenoids IV.Photooxidation of cembrene[J].Chemistry of Natural Compounds,1981,17(1):54.
-
[18]
黄申, 夏璠, 魏涛,等. 烟叶中西柏三烯-4,6-二醇降解产香菌的分离与鉴定[J].轻工学报, 2017, 32(6):73.
-
[19]
黄申,夏璠,钱玉梅,等.西柏三烯-4,6-二醇降解产香菌培养基条件优化[J].烟草科技,2017,50(3):57.
-
[20]
郑州轻工业学院.一株降解西柏三烯-4,6-二醇的新鞘氨醇杆菌及其应用:201611252017.1[P].2017-08-18.
-
[21]
HUANG S,QIAN Y M,WEI T,et al.Draft genome sequence of Novosphingobium sp.Strain HII-3,a bacterium capable of degrading the Cembranoid α(β)-2,7,11-Cembratriene-4,6-Diol to farnesal[J].Genome Announcements,2018,6(17):e00136.
-
[22]
何峰.西柏三烯-4,6-二醇的分离纯化及热裂解和烟草中HPLC分析方法研究[D].郑州:郑州轻工业学院,2015.
-
[23]
黄申,霍梦杰,钱玉梅,等.西柏三烯-4,6-二醇降解菌的筛选鉴定及其在再造烟叶中的应用[J].河南农业科学,2018,47(10):137.
-
[24]
张仁娟.消费者吸烟行为的卷烟感官评吸研究[J].中国高新区,2017(10):184.
-
[1]
-
点击查看大图
计量
- PDF下载量: 27
- 文章访问数: 1331
- 引证文献数: 0
下载:
