甲醇蛋白联产木聚糖酶发酵培养基优化
Optimization of fermentation culture medium for methanol protein co-production xylanase
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摘要: 为将实验室诱变选育出的高产甲醇蛋白毕赤酵母菌高效表达木聚糖酶，在100 L发酵罐中，对影响木聚糖酶发酵水平的培养基组分及其质量浓度等因素进行考查，初步确定培养基配方，并设计正交试验优化发酵培养基.结果表明：最优发酵培养基配方为H3PO4质量浓度25 g/L、K2HPO4质量浓度0.5 g/L、NH4Cl质量浓度0.30 g/L、CaSO4· 2H2O质量浓度0.25 g/L、NaCl质量浓度0.6 g/L、K2SO4质量浓度3.0 g/L、MgSO4· 7H2O质量浓度2.2 g/L；在最优培养基配方下，菌体湿重达到409~417 g/L，木聚糖酶活力达到40 915~41 428 U/mL.Abstract: In order to produce the xylanase efficiently, the effects of the culture medium composition and content in a 100 L fermentation tank on the xylanase fermentation were investigated with the high-yield methanol protein strain of Pichia pastoris selected by mutagenesis in our lab. The ratio of the medium was determined. Then, the orthogonal experimental scheme was designed to optimize the ratio of the medium. The result showed that the optimal components of fermentation medium were mass concentration of phosphoric acid of 25 g/L, dipotassium hydrogen phosphate of 0. 5 g/L, ammonium chloride of 0. 3 g/L, calcium sulfate dihydrate of 0. 25 g/L, sodium chloride of 0. 6 g/L, potassium sulfate of 3. 0 g/L, and magnesium sulfate heptahydrate of 2. 2 g/L; under the optimal medium formula, the wet weight of fermentation reached 409~417 g/L, and the xylanase activity reached 40 915~41 428 U/mL.
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Key words:
- methanol protein /
- xylanase /
- Pichia pastoris /
- fermentation culture medium
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