甲醇蛋白联产木聚糖酶发酵培养基优化

苟万晓; 范延超; 胡元森; 任晓辉

doi:10.12187/2021.04.001

September 2021

Article Contents

GOU Wanxiao, FAN Yanchao, HU Yuansen and et al. Optimization of fermentation culture medium for methanol protein co-production xylanase[J]. Journal of Light Industry, 2021, 36(4): 1-8. doi: 10.12187/2021.04.001

Citation: GOU Wanxiao, FAN Yanchao, HU Yuansen and et al. Optimization of fermentation culture medium for methanol protein co-production xylanase[J]. Journal of Light Industry, 2021, 36(4): 1-8. doi: 10.12187/2021.04.001 shu

Optimization of fermentation culture medium for methanol protein co-production xylanase

1. Coal Biochemical Technology Engineering Co., Ltd. of Yima Coal Industry Group, Yima 472300, China;
2. College of Bioengineering, He'nan University of Technology, Zhengzhou 450001, China

Received Date: 2020-10-19
Accepted Date: 2021-03-21

Abstract

In order to produce the xylanase efficiently, the effects of the culture medium composition and content in a 100 L fermentation tank on the xylanase fermentation were investigated with the high-yield methanol protein strain of Pichia pastoris selected by mutagenesis in our lab. The ratio of the medium was determined. Then, the orthogonal experimental scheme was designed to optimize the ratio of the medium. The result showed that the optimal components of fermentation medium were mass concentration of phosphoric acid of 25 g/L, dipotassium hydrogen phosphate of 0. 5 g/L, ammonium chloride of 0. 3 g/L, calcium sulfate dihydrate of 0. 25 g/L, sodium chloride of 0. 6 g/L, potassium sulfate of 3. 0 g/L, and magnesium sulfate heptahydrate of 2. 2 g/L; under the optimal medium formula, the wet weight of fermentation reached 409~417 g/L, and the xylanase activity reached 40 915~41 428 U/mL.
- methanol protein,
- xylanase,
- Pichia pastoris,
- fermentation culture medium
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沈阳化工大学材料科学与工程学院沈阳 110142

Optimization of fermentation culture medium for methanol protein co-production xylanase

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