JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 36 Issue 5
November 2021
Article Contents
LONG Zhangde, SU Zan, LI Jigang, et al. A mixed microbial fermentation technology for improving the quality of tobacco leaves in Baise, Guangxi[J]. Journal of Light Industry, 2021, 36(5): 59-66. doi: 10.12187/2021.05.008
Citation: LONG Zhangde, SU Zan, LI Jigang, et al. A mixed microbial fermentation technology for improving the quality of tobacco leaves in Baise, Guangxi[J]. Journal of Light Industry, 2021, 36(5): 59-66. doi: 10.12187/2021.05.008 shu

A mixed microbial fermentation technology for improving the quality of tobacco leaves in Baise, Guangxi

  • Received Date: 2021-01-18
  • Based on the fermentation pretreatment of B3F tobacco leaves in Baise, Guangxi by Saccharomyces cerevisiae, the tobacco leaves were further fermented by oligotrophic maltophilia. The optimum fermentation conditions were determined by single factor test and orthogonal test; The changes of conventional chemical components and aroma components of B3F tobacco leaves in Baise, Guangxi before and after mixed microbial fermentation were compared and analyzed, and the improvement level of sensory evaluation quality of B3F tobacco leaves after fermentation was studied. The results showed that the optimum fermentation conditions of oligotrophic maltophilia were inoculation amount of 8 ml, fermentation temperature of 34℃, fermentation time of 60 h and humidity of 70%. Under these conditions, the mass fraction of petroleum ether extract of fermented tobacco leaves reached 16.50%. After mixed microbial fermentation, the contents of total sugar and reducing sugar in conventional chemical components of tobacco leaves increased, and the sugar alkali ratio of tobacco leaves increased from 6.77 to 7.55. The mass concentration of six important aroma components in tobacco leaves (megaketene, damascus, eggplant, dihydrodamadone, vanillin and dihydrokiwifruit lactone) increased by 6.65% compared with that before fermentation. After fermentation, the aroma quality and aroma plumpness of tobacco leaves increased, and the irritation and miscellaneous gas of tobacco leaves decreased.
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    1. [1]

      ENGLISH C F, BELL E J, BERGER A J. Isolation of thermophiles from broad leaf tobacco and effect of pure culture inoculation on cigar aroma and mildness[J].Applied Microbiology,1967,15(1):117.

    2. [2]

      黄晓春.降碱增香微生物制剂改善烟叶品质研究与应用[D].长沙:湖南农业大学, 2010.

    3. [3]

      赵铭钦.烤烟陈化的生理生化机制与叶面优势微生物的分离筛选及增香效应[D].郑州:河南农业大学, 2009.

    4. [4]

      庹有朋,王刚,段旺军,等.利用生物技术对烤烟发酵的研究[J].食品与发酵科技, 2020, 56(2):69.

    5. [5]

      单宏英.陈化烟叶表面有益微生物的分离筛选、鉴定及应用研究[D].杨凌:西北农林科技大学,2012.

    6. [6]

      魏涛,陈顺心,黄申,等.β-胡萝卜素降解菌HC-3发酵条件优化及其对再造烟叶浓缩液增香效果研究[J].轻工学报, 2020, 35(2):24.

    7. [7]

      胡腾飞,焦凯旋,黄启蒙,等.固态发酵产香菌株筛选分析研究[J].农产品加工,2020,4:52.

    8. [8]

      颜克亮,武怡,曾晓鹰,等.基于提质减害的烟叶醇化技术研究进展[J].湖北农业科学, 2011, 50(3):450.

    9. [9]

      DAI J C, DONG A J, XIONG G X, et al. Production of highly active extracellular amylase and cellulase from Bacillus subtilis ZIM3 and a recombinant strain with a potential application in tobacco fermentation[J].Frontiers in Microbiology, 2020, 11:1539.

    10. [10]

      巩效伟, 段焰青, 汪显国, 等. 产香微生物复合处理提升梗丝品质的研究[J].云南农业大学学报(自然科学), 2016, 31(5):862.

    11. [11]

      帅瑶.微生物发酵口含烟原料提质增香的研究[D].贵阳:贵州大学, 2020.

    12. [12]

      WEI T, JIA B L, HUANG S, et al. Purification and characterization of a novel β-carotene-9',10'-oxygenase from Saccharomyces cerevisiae ULI3[J].Biotechnology Letter, 2015, 37(10):1993.

    13. [13]

      郑州轻工业学院. 一株嗜麦芽寡养单胞菌及其应用:201710804907.7[P]. 2019-11-22.

    14. [14]

      贾蓓蕾.α-胡萝卜素降解产香菌株的分离鉴定、发酵条件优化及酶特性研究[D].郑州:郑州轻工业学院, 2015.

    15. [15]

      邵金良, 黎其万, 刘宏程, 等. 烟草中石油醚提取物测定方法改进[J].中国烟草学报, 2010, 31(1):41.

    16. [16]

      国家烟草专卖局. 烟草及烟草制品植物碱的测定连续流动法:YC/T 160-2002[S].北京:中国标准出版社,2002.

    17. [17]

      国家烟草专卖局. 烟草及烟草制品总氮的测定连续流动法:YC/T 161-2002[S].北京:中国标准出版社,2002.

    18. [18]

      国家烟草专卖局.烟草及烟草制品钾的测定连续流动法:YC/T 217-2007[S].北京:中国标准出版社,2007.

    19. [19]

      国家烟草专卖局.烟草及烟草制品氯的测定连续流动法:YC/T 162-2011[S].北京:中国标准出版社,2011.

    20. [20]

      尹建雄,卢红,谢强,等.3, 5-二硝基水杨酸比色法快速测定烟草水溶性总糖、还原糖及淀粉的探讨[J].云南农业大学学报(自然科学),2007,22(6
      ):829.

    21. [21]

      国家烟草专卖局.烟草在制品感官评价方法:YC/T 415-2011[S].北京:中国标准出版社, 2011.

    22. [22]

      胡志忠,姜宇,刘鸿,等.利用产香酵母发酵技术改善烟叶品质[J].食品与机械,2018,34(11):200.

    23. [23]

      陈笃建.产香酵母对低次烟叶吸食品质的影响[J].贵州农业科学,2013,41(4):44.

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