微高压CO<sub>2</sub>对<i>Pseudomonas deceptionensis</i> CM2的杀菌效应研究

张艺林; 刘静飞; 任梦梦; 牛力源; 张志坚

doi:10.12187/2022.05.003

October 2022

Article Contents

ZHANG Yilin, LIU Jingfei, REN Mengmeng, et al. The bactericidal effect of petit-high pressure CO2 on Pseudomonas deceptionensis CM2[J]. Journal of Light Industry, 2022, 37(5): 24-31. doi: 10.12187/2022.05.003

Citation: ZHANG Yilin, LIU Jingfei, REN Mengmeng, et al. The bactericidal effect of petit-high pressure CO2 on Pseudomonas deceptionensis CM2[J]. Journal of Light Industry, 2022, 37(5): 24-31. doi: 10.12187/2022.05.003 shu

The bactericidal effect of petit-high pressure CO₂ on Pseudomonas deceptionensis CM2

ZHANG Yilin ¹ ,
LIU Jingfei ¹ ,
REN Mengmeng ¹ ,
NIU Liyuan ^1,2,3 ,
ZHANG Zhijian ^1,2,3

1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. He'nan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China;
3. Collaborative Innovation Center of Food Production and Safety of He'nan Province, Zhengzhou 450001, China

Received Date: 2021-10-21
Accepted Date: 2022-03-21

Abstract

This paper aimed to investigate the bactericidal effect of petit-high pressure carbon dioxide (p-HPCD) against Pseudomonas deceptionensis CM2 isolated from chicken as a function of CO₂ pressure or process time. The results showed that the bactericidal effect of p-HPCD on P.deceptionensis CM2 gradually enhanced with increasing CO₂ pressure or prolonging process time. After p-HPCD treatment under 1.3 MPa at 25 ℃ for 2 h, 4 h and 6 h, the populations of P.deceptionensis CM2 were decreased by 1.48 log₁₀ CFU/mL, 3.11 log₁₀ CFU/mL and 3.55 log₁₀ CFU/mL, respectively. p-HPCD treatment under 1.3 MPa at 25 ℃ for 4 h resulted in obvious shrinkage and cracks on the surface of P.deceptionensis CM2 cells. Moreover, p-HPCD treatment caused the leakage of nucleic acids and proteins from P.deceptionensis CM2 cells. Cell membrane permeability and membrane potential also significantly changed after p-HPCD treatment.
- petit-high pressure CO₂ bactericidal technology,
- Pseudomonas deceptionensis CM2,
- bactericidal effect
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