JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响

王璐 ,  范伊琳 ,  王子朝 ,  郭佳源 ,  王琦 ,  牛梦 ,  师怡宁

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王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
引用本文: 王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu
WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
Citation: WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu

Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响

  • 1. 河南工业大学 生物工程学院, 河南 郑州 450001;

  • 2. 华商国际工程有限公司, 北京 100069;

  • 3. 河南工业大学 国家工程实验室/省重点实验室, 河南 郑州 450001;

  • 4. 河南工业大学 国际教育学院, 河南 郑州 450001

    • 作者简介: 王璐(1998—),女,黑龙江省齐齐哈尔市人,河南工业大学硕士研究生,主要研究方向为微生物学。E-mail:wangl201906@163.com;

  • 基金项目: 河南工业大学青年骨干教师培育计划项目(21420116)
    河南工业大学粮油食品学科国家工程实验室/省重点实验室项目(NL2021006)
    河南省自然科学基金项目(212300410131)

  • 中图分类号: TS201.1

Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice

  • 1. School of Biological Engineering, He'nan University of Technology, Zhengzhou 450001, China;

  • 2. Huashang International Engineering Co., Ltd., Beijing 100069, China;

  • 3. National Engineering Laboratory/Key Laboratory of He'nan Province, He'nan University of Technology, Zhengzhou 450001, China;

  • 4. School of International Education, He'nan University of Technology, Zhengzhou 450001, China

  • Received Date: 2022-01-24

    CLC number: TS201.1

  • 摘要: 以绞股蓝内生真菌CGMCC 6882(Chaetomium globosum CGMCC 6882)发酵所产黄酮类化合物为研究对象,通过检测小鼠生长情况、体重、血清生化指标、肠道菌群组成及代谢物短链脂肪酸(SCFAs),探究其对小鼠生长和肠道菌群的影响。结果表明:饲养21 d后,空白对照组小鼠净增体重为9.5 g,实验组小鼠净增体重为7.2 g,血清生化指标无明显变化;随饲养时间的延长,实验组小鼠Ace指数、Chao1指数与Shannon指数均上升、Simpson指数下降,OTU数也高于对照组;对照组小鼠肠道中Firmicutes/Bacteroidetes(F/B)值均在0.74以上,而实验组小鼠F/B值在0.41以下,且实验组小鼠肠道中乙酸、丙酸、丁酸和总酸含量分别由22.1 μmol/g、9.3 μmol/g、8.1 μmol/g和39.5 μmol/g上升至29.3 μmol/g、15.2 μmol/g、9.5 μmol/g和54.0 μmol/g。因此,C.globosum CGMCC 6882发酵所产黄酮类化合物可以有效降低小鼠体重、提高肠道菌群多样性和丰度、增加肠道中SCFAs含量。
    Abstract: Flavonoids produced by C.globosum CGMCC 6882 was used to detect the effects on mice gut microbiota via analyzing the growth profile, body weight, serum indexes, gut microbiota composition and short-chain fatty acids (SCFAs). Results showed that after 21 days feeding, body weight gain of mice in control group was 9.5 g and the body weight gain in experimental group was 7.2 g, and there was no significant change in serum biochemical indexes. Compared to control group, Ace, Chao1 and Shannon indexes and OTU number in flavonoids group increased with the extension of feeding time, but Simpson index decreased. In addition, the values of Firmicutes/Bacteroidetes (F/B) in control were higher than 0.74, while the F/B values of flavonoids group were lower than 0.41. Furthermore, acetate, propionate, butyrate and total short chain fatty acids in the cecum contents of flavonoids group mice increased from 22.1 μmol/g, 9.3 μmol/g, 8.1 μmol/g and 39.5 μmol/g to 29.3 μmol/g, 15.2 μmol/g, 9.5 μmol/g and 54.0 μmol/g, respectively. This work indicated that flavonoids produced by C.globosum CGMCC 6882 could not only effectively reduce the body weight of mice, but also increase the diversity, abundance and SCFAs contents of gut microbiota of mice.
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王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
引用本文: 王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu
WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
Citation: WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu

Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响

    作者简介:王璐(1998—),女,黑龙江省齐齐哈尔市人,河南工业大学硕士研究生,主要研究方向为微生物学。E-mail:wangl201906@163.com
  • 1. 河南工业大学 生物工程学院, 河南 郑州 450001;
  • 2. 华商国际工程有限公司, 北京 100069;
  • 3. 河南工业大学 国家工程实验室/省重点实验室, 河南 郑州 450001;
  • 4. 河南工业大学 国际教育学院, 河南 郑州 450001
  • 收稿日期: 2022-01-24
基金项目:  河南工业大学青年骨干教师培育计划项目(21420116)河南工业大学粮油食品学科国家工程实验室/省重点实验室项目(NL2021006)河南省自然科学基金项目(212300410131)

摘要: 以绞股蓝内生真菌CGMCC 6882(Chaetomium globosum CGMCC 6882)发酵所产黄酮类化合物为研究对象,通过检测小鼠生长情况、体重、血清生化指标、肠道菌群组成及代谢物短链脂肪酸(SCFAs),探究其对小鼠生长和肠道菌群的影响。结果表明:饲养21 d后,空白对照组小鼠净增体重为9.5 g,实验组小鼠净增体重为7.2 g,血清生化指标无明显变化;随饲养时间的延长,实验组小鼠Ace指数、Chao1指数与Shannon指数均上升、Simpson指数下降,OTU数也高于对照组;对照组小鼠肠道中Firmicutes/Bacteroidetes(F/B)值均在0.74以上,而实验组小鼠F/B值在0.41以下,且实验组小鼠肠道中乙酸、丙酸、丁酸和总酸含量分别由22.1 μmol/g、9.3 μmol/g、8.1 μmol/g和39.5 μmol/g上升至29.3 μmol/g、15.2 μmol/g、9.5 μmol/g和54.0 μmol/g。因此,C.globosum CGMCC 6882发酵所产黄酮类化合物可以有效降低小鼠体重、提高肠道菌群多样性和丰度、增加肠道中SCFAs含量。

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