JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 37 Issue 6
December 2022
Article Contents
SHEN Fangming, YIN Shutao and ZHAO Chong. Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells[J]. Journal of Light Industry, 2022, 37(6): 50-57. doi: 10.12187/2022.06.007
Citation: SHEN Fangming, YIN Shutao and ZHAO Chong. Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells[J]. Journal of Light Industry, 2022, 37(6): 50-57. doi: 10.12187/2022.06.007 shu

Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells

  • Received Date: 2022-05-30
  • In this study, the co-culture system of Caco-2 and HaCaT cells was established, and the inhibitory effect of punicalagin on cyclobutene pyrimidine dimers (CPD) which was one major type of UVB-induced DNA lesions was evaluated by immunofluorescence. Then the effects of punicalagin on transcription and expression levels of nucleotide excision repair (NER) related genes was further investigated by quantitative real-time PCR, immunoprecipitation and western-blotting. The results showed that punicalagin reduced CPD level in HaCaT cells, upregulated xeroderma pigmentosum group C (XPC) mRNA level and attenuated XPA acetylation in a SIRT1-dependent manner. This showed, punicalagin's efficacy against UVB-induced photodamage in the co-culture system of Caco-2 and HaCaT cells was probably activated by the NER related XPC/XPA which depended on the deacetylation activity of SIRT1.
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