JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 1
February 2023
Article Contents
WANG Anna, WANG Xuebing and WU Ligen. Effect of ionic concentration on the interaction between gelatin and amylose[J]. Journal of Light Industry, 2023, 38(1): 1-9. doi: 10.12187/2023.01.001
Citation: WANG Anna, WANG Xuebing and WU Ligen. Effect of ionic concentration on the interaction between gelatin and amylose[J]. Journal of Light Industry, 2023, 38(1): 1-9. doi: 10.12187/2023.01.001 shu

Effect of ionic concentration on the interaction between gelatin and amylose

  • Received Date: 2022-06-07
  • The effect of ionic concentration on the interaction between gelatin and amylose was investigated by spectroscopic methods. The results showed that ionic concentration could change the hydrophobic microenvironment of gelatin. With different ionic concentration, amylose could quench the endogenous fluorescence of gelatin to different degrees. The quenching mechanism was coupling quenching, which was mainly static quenching. With the increase of ionic concentration, the binding ability of gelatin and amylose was enhanced. In aqueous solution, the forces between gelatin and amylose were mainly van der Waals forces and hydrogen bonds, which were mainly driven by hydrophobic forces after sodium chloride was added. Gelatin-amylose binding caused rearrangement and conformational changes of gelatin, resulting in the change of tyrosine residues and tryptophan residues, and the binding site was closer to the tyrosine residues. The gelatin-amylose interaction was effected by the ionic concentration, the addition of sodium chloride was beneficial to the combination of gelatin and amylose and caused the change of conformation of gelatin.
  • 加载中
    1. [1]

      WANG K, WANG W H, YE R, et al.Mechanical and barrier properties of maize starch-gelatin composite films:Effects of amylose content[J].Journal of the Science of Food and Agriculture, 2017, 97(11):3613-3622.

    2. [2]

      姜艳敏.明胶对面团及其发酵制品品质的影响[D].郑州:河南工业大学, 2021.

    3. [3]

      余文杰.猪皮明胶对冷冻面团及其面包品质的影响与机理研究[D].无锡:江南大学, 2020.

    4. [4]

      ANASTASIA B, PERSEPHONI G.Evaluation of biodegradable gelatin and gelatin:Rice starch coatings to fresh cut zucchini slices[J].Horticulturae, 2022, 8(11):1031.

    5. [5]

      SANG S Y, OU C G, FU Y Q, et al.Complexation of fish skin gelatin with glutentin and its effect on the properties of wheat dough and bread[J].Food Chemistry, 2022, 14:100319.

    6. [6]

      CHEN G J, EHMKE L, MILLER R, et al.Effect of sodium chloride and sodium bicarbonate on the physicochemical properties of soft wheat flour doughs and gluten polymerization[J].Journal of Agricultural and Food Chemistry, 2018, 66(26):6840-6850.

    7. [7]

      饶毅, 胡良豪, 梁浩, 等.电场作用对明胶溶液性质影响研究[J].中国皮革, 2011, 40(13):11-13.

    8. [8]

      CHAVES O A, PIRES L O, CASTRO R N, et al.Theoretical and experimental studies of the interaction between human serum albumin and artepillin C, an active principle of the brazilian green propolis[J].Revista Virtualde Quimica, 2019, 11(5):1561-1577.

    9. [9]

      CHI Z X, LIU R T.Phenotypic characterization of the binding of tetracycline to human serum albumin[J].Biomacromolecules, 2011, 12(1):203-209.

    10. [10]

      GENIS D O, BILGE G, SEAER B, et al.Identification of cow, buffalo, goat and ewe milk species in fermented dairy products using synchronous fluorescence spectroscopy[J].Food Chemistry, 2019, 284:60-66.

    11. [11]

      丁小娟.嗜酸乳杆菌β-葡萄糖苷酶及其与槲皮素糖苷底物作用研究[D].贵阳:贵州大学, 2019.

    12. [12]

      WLODARSKQ K, KHMELINSKILL I, SIKORSKQ E.Authentication of apple juice categories based on multivariate analysis of the synchronous fluorescence spectra[J].Food Control, 2018, 86:42-49.

    13. [13]

      钟姝凝.β-葡萄糖苷酶对人参皂苷生物转化及其相互作用机制研究[D].长春:吉林大学, 2021.

    14. [14]

      MA Y L, LI S Y, JI T T, et al.Development and optimization of dynamic gelatin/chitosan nanoparticles incorporated with blueberry anthocyanins for milk freshness monitoring[J].Carbohydrate Polymers, 2020, 247:116738.

    15. [15]

      关姣.模拟生理环境下多酚化合物与血清白蛋白的相互作用及对蛋白性质的影响[D].天津:天津大学, 2019.

    16. [16]

      王启明, 唐瑜婉, 李春翼, 等.NaCl浓度对麦醇溶蛋白与槲皮素相互作用的影响[J].食品科学, 2021, 42(8):29-39.

    17. [17]

      HASNI I, BOURASSA P, HANDANI S, et al.Interaction of milk α- and β-caseins with tea polyphenols[J].Food Chemistry, 2011, 126(2):630-639.

    18. [18]

      XIA W Y, MA L, CHEN X K, et al.Physicochemical and structural properties of composite gels prepared with myofibrillar protein and lecithin at various ionic strengths[J].Food Hydrocolloids, 2018, 82:135-143.

    19. [19]

      陈益时.量子点荧光猝灭-恢复法的构建及其在蛋白质检测中的应用[D].武汉:武汉大学, 2020.

    20. [20]

      赵倩.基于分子相互作用研究蛋白淀粉消化及多酚抗氧化的影响[D].镇江:江苏大学, 2020.

    21. [21]

      KUIJPERS K P L, BOTTECHIA C, CAMBIE D, et al. A fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis[J].Angewandte Chemie Tnt Ed Engl, 2018, 57(35):11278-11282.

    22. [22]

      QIN Y, ZHANG Y X, YAN S L, et al.A comparison study on the interaction of hyperoside and bovine serum albumin with Tachiya model and Stern-Volmer equation[J].Spectrochimica Acta Part A(Molecular and Biomolecular Spectroscopy), 2010, 75(5):1506-1510.

    23. [23]

      任国艳, 孙贺, 樊金玲, 等.荧光光谱法和分子对接模拟技术研究白藜芦醇与胃蛋白酶的相互作用[J].光谱学与光谱分析, 2019, 39(4):1103-1108.

    24. [24]

      JIANG J, ZHANG Z P, ZHAO J, et al.The effect of non-covalent interaction of chlorogenic acid with whey protein and casein on physicochemical and radical-scavenging activity of in vitro protein digests[J].Food Chemistry.2018, 268:334-341.

    25. [25]

      YANG Z H, CHENG X D, LI X R.Investigation of the interaction between human hemoglobin and five antioxidants by fluorescence spectroscopy and molecular modeling[J].Journal of the Iranian Chemical Society, 2018, 15(2):245-257.

    26. [26]

      DANKOWSKA A, MALECKA M, KOWALEWSKI W.Application of synchronous fluorescence spectroscopy with multivariate data analysis for determination of butter adulteration[J].International Journal of Food Science & Technology, 2014, 49(12):2628-2634.

    27. [27]

      方甜, 廖家乐, 范艳丽, 等.枸杞叶黄酮与不同蛋白质的相互作用及体外抗氧化活性比较[J].安徽农业大学学报, 2022, 49(1):165-174.

    28. [28]

      齐宝坤, 江连洲, 王欢, 等.离子强度对大豆11S球蛋白表面疏水性及结构的影响[J].食品科学, 2018, 39(8):39-44.

Article Metrics

Article views(2871) PDF downloads(60) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return