基于宏基因组学的霍氏肠杆菌发酵解析及其烟叶品质提升机制研究

王文婷; 宋凯; 杨晨; 臧志鹏; 王祯; 陈梦月; 刘晨; 翟玉俊

doi:10.12187/2023.01.010

February 2023

Article Contents

WANG Wenting, SONG Kai, YANG Chen, et al. Macrogenomics-based investigation of the mechanism underlying tobacco fermentation by Enterobacter hormaechei[J]. Journal of Light Industry, 2023, 38(1): 79-89. doi: 10.12187/2023.01.010

Citation: WANG Wenting, SONG Kai, YANG Chen, et al. Macrogenomics-based investigation of the mechanism underlying tobacco fermentation by Enterobacter hormaechei[J]. Journal of Light Industry, 2023, 38(1): 79-89. doi: 10.12187/2023.01.010 shu

Macrogenomics-based investigation of the mechanism underlying tobacco fermentation by Enterobacter hormaechei

1. Technology Center, China Tobacco Gansu Industrial Co., Ltd., Lanzhou 730050, China;
2. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2022-10-12

Abstract

The dynamic changes of surface microorganisms were monitored during the fermentation of tobacco by Enterobacter hormaechei F8-1, and mechanism of tobacco fermentation by F8-1 was studied combined with neutral aroma components of tobacco leaves and sensory analysis. The results showed that after fermentation the aroma quality and sweet taste were improved, the offensive odor and irritation were reduced. The contents of megastigmatrienone, solanone, (E)-β-damascenone and dihydrodamascenone were increased respectively by 37.40%, 59.87%, 53.02% and 46.61%. During the fermentation, the predominant species were E hormaechei F8-1, Pseudomonas aeruginosa, and Enterobacter cloacae; the highest number of glycoside hydrolases accounted for 78.3% of the microbial carbohydrate-active enzymes on the surface of the tobacco filaments during fermentation; the abundance of Enterobacter spp. was positively correlated with the content of solanone, megastigmatrienone, (E)-β-damascenone and dihydrodamascenone, and the abundance of glycoside hydrolase family 1 (GH1) was positively correlated with the content of solanone, megastigmatrienon and farnesylacetone. Glycoside hydrolases in the GH1 might be key factors for sensory enhancement after tobacco fermentation.
- metagenomics,
- neutral aroma component,
- Enterobacter hormaechei F8-1,
- glucoside hydrolase
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Macrogenomics-based investigation of the mechanism underlying tobacco fermentation by Enterobacter hormaechei

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