JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 1
February 2023
Article Contents
LI Shitou, PAN Fanda, HUANG Xiaoyu, et al. Screening of tobacco-derived functional microorganisms with different aging time based on metagenomics[J]. Journal of Light Industry, 2023, 38(1): 101-109. doi: 10.12187/2023.01.012
Citation: LI Shitou, PAN Fanda, HUANG Xiaoyu, et al. Screening of tobacco-derived functional microorganisms with different aging time based on metagenomics[J]. Journal of Light Industry, 2023, 38(1): 101-109. doi: 10.12187/2023.01.012 shu

Screening of tobacco-derived functional microorganisms with different aging time based on metagenomics

  • Received Date: 2022-10-08
  • The community structure and functional gene succession of surface microorganisms in Yunnan tobacco leaves at different aging stages were analyzed based on metagenomics. The changes of neutral aroma components during the aging of tobacco leaves were traced. The correlation between microorganisms, enzyme gene and neutral aroma components was explored, and a screening method for tobacco-derived functional microorganisms was established. The results showed at the 3rd, 6th, 9th and 12th month of aging, the dominant genera were Sphingomonas, Enterobacter, Pantoea, and Acinetobacter,respectively. The neutral aroma components in tobacco leaves such as benzyl alcohol, phenylacetaldehyde, 2-acetylpyrrole, solanone, damascenone and megastigmatrienone had an increasing trend. Furfural and β-cyclocitral had a first increase and then decrease trend. The content of hydrocarbons decreased. Klebsiella had a significant positive correlation with β-damascenone, megastrienone and solanone. Acinetobacter had a significant positive correlation with 2-acetylfuran, linalool and megastrienone. Phenylethylaldehyde, benzaldehyde, furfuryl alcohol and cypressene were negatively correlated with almost all carbohydrate synthase, and most glycosidase families were positively correlated with neutral aroma components. A total of 75 strains of microorganisms were screened during tobacco leaf aging, and the microbial types and functions were basically consistent with the results of metagenome analysis, indicating that the analysis results of metagenome could guide microbial screening, enhance the purpose of screening, and improve screening efficiency.
  • 加载中
    1. [1]

      赵勇, 黄劲松, 宋新蕊, 等.宏基因组的生物信息分析[J].生物信息学, 2013(4):282-286.

    2. [2]

      CHEN K, PACHTER L.Bioinformatics for whole-genome shotgun sequencing of microbial communities[J].PLoS Computational Biology, 2005, 1(2):106-112.

    3. [3]

      RUZA A, DENISE T, ANNIKA L, et al.Comparison of targeted next-generation sequencing and Sanger sequencing for the detection of PIK3CA mutations in breast cancer[J].BMC Clinical Pathology, 2015(15):1-9.

    4. [4]

      DIEHL G E, LONGMAN R S, ZHANG J X, et al.Micro-biota restricts trafficking of bacteria to mesenteric lymph nodes by CX(3)CR1(hi) cells[J].Nature, 2013, 494(7435):116-120.

    5. [5]

      WANG F, ZHAO H W, XIANG H Y, et al.Species diversity and functional prediction of surface bacterial communities on aging flue-cured tobaccos[J].Current Microbiology, 2018, 75(10):1306-1315.

    6. [6]

      TYX R E, STANFILL S B, KEONG L M, et al.Characterization of bacterial communities in selected smokeless tobacco products using 16S rDNA analysis[J].PLoS ONE, 2016, 11(1):e0146939.

    7. [7]

      常安然, 李佳, 张耸, 等.基于宏基因组学16S rDNA测序对烟草根际土壤细菌群落组成分析[J].中国农业科技导报, 2017, 19(2):43-50.

    8. [8]

      赵铭钦, 刘云, 李芳芳, 等.陈化烤烟叶面优势菌的筛选鉴定与其增香效应[J].微生物学报, 2009, 49(5):625-631.

    9. [9]

      王卫峰, 章友爱, 陈子枫, 等.自然醇化对烟叶化学组分及致香物质的影响[J].广西农学报, 2016, 31(2):8-13.

    10. [10]

      刘洋.烟叶增香提质微生物的筛选及其应用[D].武汉:华中农业大学, 2011.

    11. [11]

      李秀妮, 李猛, 万德建, 等.烟叶微生物及其在烟叶发酵和醇化中的作用研究进展[J].微生物学通报, 2019, 46(6):1520-9.

    12. [12]

      单宏英.陈化烟叶表面有益微生物的分离筛选、鉴定及应用研究[D].咸阳:西北农林科技大学, 2012.

    13. [13]

      马英.烟叶表面高效降解蛋白质的芽孢杆菌的分离筛选及其鉴定[J].喀什师范学院学报, 2012, 18(1):101-104.

    14. [14]

      HOLGER Z, SABINE L, MANUELA S, et al.A peroxidase from Lepista irina cleaves β, β-carotene to flavor compounds[J].Biological Chemistry, 2003, 384(7):1049-1056.

    15. [15]

      李秀红, 李冰, 李仙, 等.一株产香微生物的筛选[C]//中国烟草学会.中国烟草学会工业专业委员会烟草化学学术研讨会论文集.海口:[出版社不详], 2005:420-430.

    16. [16]

      陈竹亭.陈化进程中烟叶表面细菌组成的变化以及果胶质降解菌群的分离[D].南京:南京大学, 2012.

    17. [17]

      孙政.降低烟草亚硝胺目的菌的筛选及应用研究[D].武汉:华中农业大学, 2017.

    18. [18]

      杨应美, 卢灿华, 罗朝旺, 等.烟草黑胫病生防菌筛选及对根际土壤微生物群落的影响[J].西南农业学报, 2022, 35(4):790-796.

    19. [19]

      贺兆伟, 奚家勤, 邓宾玲, 等.烟叶中高产果胶酶菌株的筛选及酶学性质[J].烟草科技, 2013(5):72-76.

    20. [20]

      黄申, 马宁, 王琼波, 等.再造烟叶浓缩液增香菌的筛选、鉴定与发酵优化[J].轻工学报, 2020, 35(2):33-41.

Article Metrics

Article views(4283) PDF downloads(34) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return