JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

甾醇分子差异对脂质体结构稳定性的影响

邓莉梅 ,  刘宇佳 ,  朱杰 ,  于泓鹏

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邓莉梅, 刘宇佳, 朱杰, 等. 甾醇分子差异对脂质体结构稳定性的影响[J]. 轻工学报, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
引用本文: 邓莉梅, 刘宇佳, 朱杰, 等. 甾醇分子差异对脂质体结构稳定性的影响[J]. 轻工学报, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
shu
DENG Limei, LIU Yujia, ZHU Jie and et al. Effect of sterol molecular difference on the structural stability of liposomes[J]. Journal of Light Industry, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
Citation: DENG Limei, LIU Yujia, ZHU Jie and et al. Effect of sterol molecular difference on the structural stability of liposomes[J]. Journal of Light Industry, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
shu

甾醇分子差异对脂质体结构稳定性的影响

  • 1. 广东工业大学 轻工化工学院, 广东 广州 510006;

  • 2. 东莞理工学院 化学工程与能源技术学院/中国轻工业健康食品开发与营养调控重点实验室, 广东 东莞 523808

    • 作者简介: 邓莉梅(1996-),女,四川省资阳市人,广东工业大学硕士研究生,主要研究方向为食品递送系统。E-mail:1176993285@qq.com;

  • 基金项目: 国家自然科学基金青年基金项目(31901682)；广东省普通高校青年创新人才类项目(2018KQNCX259)；广东省创新强校工程创新团队项目(2021KCXTD035)

  • 中图分类号: TS201.1

Effect of sterol molecular difference on the structural stability of liposomes

  • 1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China;

  • 2. Key Laboratory of Healthy Food Development and Nutrition Regulation of China National Light Industry/School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China

  • Received Date: 2022-02-27

    CLC number: TS201.1

  • 摘要: 通过添加胆固醇、 β-谷甾醇和豆甾醇,采用乙醇注入法结合动态高压微流射技术制备甾醇脂质体,研究不同甾醇对脂质体膜结构稳定性的影响。结果表明:β-谷甾醇和豆甾醇的加入,使甾醇脂质体粒径从(48.35±0.41)nm分别增至(106.27±0.90)nm和(107.27±0.59)nm,Zeta电位保持不变,均在-30 mV左右;电子显微镜观察发现,添加甾醇的脂质体形成了稳定的磷脂双分子层结构;甾醇可以使脂质体的盐稳定性和pH稳定性增强,但对温度稳定性无显著影响;β-谷甾醇和豆甾醇的加入使脂质体膜的流动性、疏水性和微极性都显著减小,这表明脂质体磷脂双分子膜的结构变得更加致密。
    Abstract: Liposomes were prepared by ethanol injection combined with dynamic high-pressure microfluidic technology. The effects of different sterol molecular, including cholesterol, β-sitosterol and stigmasterol, on the membrane structural stability of liposome were compared. The results showed that the addition of β-sitosterol and stigmasterol increased the particle size of liposomes from (48.35±0.41) nm to (106.27±0.90) nm and (107.27±0.59) nm, respectively, and the Zeta potential remained unchanged at about -30 mV. Electron microscopy showed that the lipid containing sterol formed stable bilayer structure of phospholipid. The salt stability and pH stability had been enhanced, but temperature stability had no significant change. The addition of β-sitosterol and stigmasterol reduced the micropolarity, hydrophobicity and fluidity of liposome membrane, resulting in a tighter phospholipid bimolecular membrane structure in liposomes.
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邓莉梅, 刘宇佳, 朱杰, 等. 甾醇分子差异对脂质体结构稳定性的影响[J]. 轻工学报, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
引用本文: 邓莉梅, 刘宇佳, 朱杰, 等. 甾醇分子差异对脂质体结构稳定性的影响[J]. 轻工学报, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
shu
DENG Limei, LIU Yujia, ZHU Jie and et al. Effect of sterol molecular difference on the structural stability of liposomes[J]. Journal of Light Industry, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
Citation: DENG Limei, LIU Yujia, ZHU Jie and et al. Effect of sterol molecular difference on the structural stability of liposomes[J]. Journal of Light Industry, 2023, 38(2): 33-40. doi: 10.12187/2023.02.004
shu

甾醇分子差异对脂质体结构稳定性的影响

    作者简介:邓莉梅(1996-),女,四川省资阳市人,广东工业大学硕士研究生,主要研究方向为食品递送系统。E-mail:1176993285@qq.com
  • 1. 广东工业大学 轻工化工学院, 广东 广州 510006;
  • 2. 东莞理工学院 化学工程与能源技术学院/中国轻工业健康食品开发与营养调控重点实验室, 广东 东莞 523808
  • 收稿日期: 2022-02-27
基金项目:  国家自然科学基金青年基金项目(31901682)；广东省普通高校青年创新人才类项目(2018KQNCX259)；广东省创新强校工程创新团队项目(2021KCXTD035)

摘要: 通过添加胆固醇、 β-谷甾醇和豆甾醇,采用乙醇注入法结合动态高压微流射技术制备甾醇脂质体,研究不同甾醇对脂质体膜结构稳定性的影响。结果表明:β-谷甾醇和豆甾醇的加入,使甾醇脂质体粒径从(48.35±0.41)nm分别增至(106.27±0.90)nm和(107.27±0.59)nm,Zeta电位保持不变,均在-30 mV左右;电子显微镜观察发现,添加甾醇的脂质体形成了稳定的磷脂双分子层结构;甾醇可以使脂质体的盐稳定性和pH稳定性增强,但对温度稳定性无显著影响;β-谷甾醇和豆甾醇的加入使脂质体膜的流动性、疏水性和微极性都显著减小,这表明脂质体磷脂双分子膜的结构变得更加致密。

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