JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

叶惠萍 ,  曾晓房 ,  白卫东 ,  肖更生 ,  杨娟 ,  冼燕萍 ,  陈伟波 ,  董浩

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叶惠萍, 曾晓房, 白卫东, 等. 红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测[J]. 轻工学报, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
引用本文: 叶惠萍, 曾晓房, 白卫东, 等. 红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测[J]. 轻工学报, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
shu
YE Huiping, ZENG Xiaofang, BAI Weidong, et al. Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS[J]. Journal of Light Industry, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
Citation: YE Huiping, ZENG Xiaofang, BAI Weidong, et al. Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS[J]. Journal of Light Industry, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
shu

红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

  • 1. 仲恺农业工程学院 轻工食品学院, 广东 广州 510225;

  • 2. 广东省岭南特色食品科学与技术重点实验室, 广东 广州 510225;

  • 3. 农业农村部岭南特色食品绿色加工与智能制造重点实验室, 广东 广州 510225;

  • 4. 广州质量监督检测研究院, 广东 广州 510110;

  • 5. 梅州市金绿现代农业发展有限公司, 广东 梅州 514000

    • 作者简介: 叶惠萍(1997-),女,广东省东莞市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:hpye@foxmail.com;

  • 基金项目: 国家自然科学基金青年基金项目(32001729)；广东省普通高校特色创新项目(2022KTSCX058)；广东省岭南特色食品科学与技术重点实验室项目(2021B1212040013)；广东省驻镇帮镇扶村农村科技特派员项目(KTP20210224)；梅州市兴宁市肉鸽产业园技术研发项目(粤农农函〔2021〕1024号)；广东省仲恺农业工程学院-广州质量监督检测研究院联合培养研究生示范基地项目(粤教研函〔2021〕2号)；仲恺农业工程学院研究生科技创新基金项目(KJCX2022013)

  • 中图分类号: TS201.6

Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS

  • 1. College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;

  • 2. Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou 510225, China;

  • 3. Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture, Guangzhou 510225, China;

  • 4. Guangzhou Quality Supervision and Testing Institute, Guangzhou 510110, China;

  • 5. Meizhou Jinlv Modern Agriculture Development Co., Ltd., Meizhou 514000, China

  • Received Date: 2022-05-04

    CLC number: TS201.6

  • 摘要: 以市售红烧乳鸽为研究对象,建立超高效液相色谱-四极轨道高分辨质谱(UHPLC-Q-Orbitrap HRMS)联用法,并对红烧乳鸽样品中不同部位11种极性杂环胺含量进行检测。结果表明:建立的方法线性关系良好、灵敏度较高,11种杂环胺的质量准确度均＜3×10-6,决定系数R2≥0.997 2,方法的检出限为0.03~0.60 μg/kg,定量限为0.10~2.00 μg/kg;该方法具有稳定的回收率和一定的精确性,11种杂环胺的回收率为83.40%~114.80%,精密度为1.40%~7.20%,结果良好;红烧乳鸽中主要检出MeIQx(0.10~0.46 μg/kg)、PhIP(0.10~0.53 μg/kg)和DMIP(0.10~0.25 μg/kg)3种杂环胺,乳鸽部位中HAAs总含量由多到少依次为鸽跟>鸽胸>鸽翅>鸽腿。
    Abstract: With commericially available braised squab as research object, UHPLC-Q-Orbitrap HRMS was established. Eleven polar heterocyclic aromatic amines in braised squab were detected by UHPLC-Q-Orbitrap HRMS. The results showed that the method had good linearity and high mass accuracy. The mass accuracy of 11 heterocyclic aromatic amines was <3×10-6. The determination coefficients were greater than 0.997 2. The limit of detection was 0.03~0.60 μg/kg, and the limit of quantification was 0.10~2.00 μg/kg. This method had stable recovery and certain accuracy. The recovery of 11 heterocyclic aromatic amines at 3 spiked concentrations ranged from 83.40% to 114.80%, and the precision ranged from 1.40% to 7.20%. The main heterocyclic aromatic amines detected in braised squab were MeIQx (0.10~0.46 μg/kg), PhIP (0.10~0.53 μg/kg) and DMIP (0.10~0.25 μg/kg). The total content of heterocyclic aromatic amines in 4 parts of braised squab was the heel, breast, wing and leg in descending oder.
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叶惠萍, 曾晓房, 白卫东, 等. 红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测[J]. 轻工学报, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
引用本文: 叶惠萍, 曾晓房, 白卫东, 等. 红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测[J]. 轻工学报, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
shu
YE Huiping, ZENG Xiaofang, BAI Weidong, et al. Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS[J]. Journal of Light Industry, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
Citation: YE Huiping, ZENG Xiaofang, BAI Weidong, et al. Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS[J]. Journal of Light Industry, 2023, 38(2): 56-62. doi: 10.12187/2023.02.007
shu

红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

    作者简介:叶惠萍(1997-),女,广东省东莞市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:hpye@foxmail.com
  • 1. 仲恺农业工程学院 轻工食品学院, 广东 广州 510225;
  • 2. 广东省岭南特色食品科学与技术重点实验室, 广东 广州 510225;
  • 3. 农业农村部岭南特色食品绿色加工与智能制造重点实验室, 广东 广州 510225;
  • 4. 广州质量监督检测研究院, 广东 广州 510110;
  • 5. 梅州市金绿现代农业发展有限公司, 广东 梅州 514000
  • 收稿日期: 2022-05-04
基金项目:  国家自然科学基金青年基金项目(32001729)；广东省普通高校特色创新项目(2022KTSCX058)；广东省岭南特色食品科学与技术重点实验室项目(2021B1212040013)；广东省驻镇帮镇扶村农村科技特派员项目(KTP20210224)；梅州市兴宁市肉鸽产业园技术研发项目(粤农农函〔2021〕1024号)；广东省仲恺农业工程学院-广州质量监督检测研究院联合培养研究生示范基地项目(粤教研函〔2021〕2号)；仲恺农业工程学院研究生科技创新基金项目(KJCX2022013)

摘要: 以市售红烧乳鸽为研究对象,建立超高效液相色谱-四极轨道高分辨质谱(UHPLC-Q-Orbitrap HRMS)联用法,并对红烧乳鸽样品中不同部位11种极性杂环胺含量进行检测。结果表明:建立的方法线性关系良好、灵敏度较高,11种杂环胺的质量准确度均＜3×10-6,决定系数R2≥0.997 2,方法的检出限为0.03~0.60 μg/kg,定量限为0.10~2.00 μg/kg;该方法具有稳定的回收率和一定的精确性,11种杂环胺的回收率为83.40%~114.80%,精密度为1.40%~7.20%,结果良好;红烧乳鸽中主要检出MeIQx(0.10~0.46 μg/kg)、PhIP(0.10~0.53 μg/kg)和DMIP(0.10~0.25 μg/kg)3种杂环胺,乳鸽部位中HAAs总含量由多到少依次为鸽跟>鸽胸>鸽翅>鸽腿。

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