介质阻挡放电等离子体对单增李斯特菌的杀灭效果及作用机制研究
Inactivation effect and mechanism of dielectric barrier discharge plasma against Listeria monocytogenes
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摘要: 采用扫描电子显微镜(SEM)、流式细胞分析、荧光染色等方法研究介质阻挡放电(Dielectric Barrier Discharge,DBD)等离子体对单增李斯特菌(Listeria monocytogenes)的杀灭效果及对其细胞形态、细胞膜变化、胞内活性氧(Reactive Oxygen Species,ROS)水平等的影响。结果表明:经放电功率为20.8 W的DBD等离子体处理80 s后,L.monocytogenes菌落数从初始8.26 lg CFU/mL降低至1.30 lg CFU/mL,其胞内ROS相对水平显著升高了9.5倍(P<0.05);DBD等离子体处理会破坏L.monocytogenes的细胞形态,增强细胞膜通透性并使其发生去极化,且细胞损伤程度随处理时间的延长而显著增加;经DBD等离子体处理后,生理盐水的pH值显著降低,氧化还原电位(ORP)、NO3-、NO-2和H2O2浓度均显著升高。DBD等离子体杀灭L.monocytogenes的作用机制是其可损伤细胞膜、诱导氧化应激损伤等。
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关键词:
- 介质阻挡放电等离子体 /
- 单增李斯特菌 /
- 杀灭效果 /
- 作用机制
Abstract: The effects of dielectric barrier discharge (DBD) plasma on the inactivation effect, the cell morphology, membrane changes and intracellular reactive oxygen species (ROS) levels of Listeria monocytogenes were investigated by scanning electron microscopy (SEM), flow cytometry, fluorescence staining and so on. The results showed that the population of L.monocytogenes was reduced from an initial level of 8.26 lg CFU/mL to 1.30 lg CFU/mL after DBD plasma treatment at 20.8 W for 80 s, and the intracellular ROS level increased by 9.5-folds (P<0.05). DBD plasma treatment caused damage in the morphology and cell membrane of L.monocytogenes in a treatment time-dependent manner. Moreover, DBD plasma caused the depolarization of the cell membrane of L.monocytogenes. In addition, after DBD plasma treatment, the pH value of normal saline decreased significantly, the oxidation-reduction potential and concentration of NO3-, NO-2 and H2O2 increased significantly. In summary, DBD plasma could inactivate L.monocytogenes by disrupting cell membranes and inducing oxidative stress damage. -
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