JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 3
June 2023
Article Contents
WANG Bohua, XUE Dong, DONG Shanshan and et al. Inactivation effect and mechanism of dielectric barrier discharge plasma against Listeria monocytogenes[J]. Journal of Light Industry, 2023, 38(3): 17-24,54. doi: 10.12187/2023.03.003
Citation: WANG Bohua, XUE Dong, DONG Shanshan and et al. Inactivation effect and mechanism of dielectric barrier discharge plasma against Listeria monocytogenes[J]. Journal of Light Industry, 2023, 38(3): 17-24,54. doi: 10.12187/2023.03.003 shu

Inactivation effect and mechanism of dielectric barrier discharge plasma against Listeria monocytogenes

  • Received Date: 2022-04-25
    Accepted Date: 2022-07-24
  • The effects of dielectric barrier discharge (DBD) plasma on the inactivation effect, the cell morphology, membrane changes and intracellular reactive oxygen species (ROS) levels of Listeria monocytogenes were investigated by scanning electron microscopy (SEM), flow cytometry, fluorescence staining and so on. The results showed that the population of L.monocytogenes was reduced from an initial level of 8.26 lg CFU/mL to 1.30 lg CFU/mL after DBD plasma treatment at 20.8 W for 80 s, and the intracellular ROS level increased by 9.5-folds (P<0.05). DBD plasma treatment caused damage in the morphology and cell membrane of L.monocytogenes in a treatment time-dependent manner. Moreover, DBD plasma caused the depolarization of the cell membrane of L.monocytogenes. In addition, after DBD plasma treatment, the pH value of normal saline decreased significantly, the oxidation-reduction potential and concentration of NO3-, NO-2 and H2O2 increased significantly. In summary, DBD plasma could inactivate L.monocytogenes by disrupting cell membranes and inducing oxidative stress damage.
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