JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 5
October 2023
Article Contents
DUAN Yuying, XI Jun, WANG Yichao, et al. Prediction and primary mapping of antigenic epitopes of glycinin G3A1b[J]. Journal of Light Industry, 2023, 38(5): 42-50. doi: 10.12187/2023.05.006
Citation: DUAN Yuying, XI Jun, WANG Yichao, et al. Prediction and primary mapping of antigenic epitopes of glycinin G3A1b[J]. Journal of Light Industry, 2023, 38(5): 42-50. doi: 10.12187/2023.05.006 shu

Prediction and primary mapping of antigenic epitopes of glycinin G3A1b

  • Received Date: 2022-12-08
    Accepted Date: 2023-02-03
  • Bioinformatics software was used to predict the potential antigenic epitopes of G3 subunit A1b acidic polypeptide chain in glycinin. Three pairs of primers were designed and synthesized according to the overlapping amino acid sequence of A1b polypeptide chain, and the cloning vector of the target fragment was constructed. The target protein was expressed by phage display technology, and the heat-processed antibody was prepared and the destroyed antigenic epitopes were identified by ELISA. The results showed that G3A1b may contain 7 linear epitopes: 27QQN29,39LKPDNRIE46,58NNK60,114PQQKGQSSRP123,134R, 174QMPR177,184NQEQEF189. The size of the target gene matched expectations,the cloning vector was successfully constructed and the phage was successfully assembled. G3A1b-3 fragment had high antigenicity and was most obviously destroyed in the process. The results provide theoretical support for the precise positioning of the key amino acids in glycinin G3A1b.
  • 加载中
    1. [1]

      KEREZSI A D,JACQUET N,BLECKER C.Advances on physical treatments for soy allergens reduction:A review[J].Trends in Food Science & Technology,2022,122:24-39.

    2. [2]

      WANG J,HE Z Y,RAGHAVAN V.Soybean allergy: Characteristics, mechanisms, detection and its reduction through novel food processing techniques[J].Critical Reviews in Food Science and Nutrition,2022,11-14.

    3. [3]

      DICK K,PATTANG A,HOOKER J,et al.Human-soybean allergies: Elucidation of the seed proteome and comprehensive protein-protein interaction prediction[J].Journal of Proteome Research,2021,20(11):4925-4947.

    4. [4]

      ZHENG S G,QIN G X,JING C,et al.Acidic polypeptides A(1a), A(3) and A(4) of Gly m 6 (glycinin) are allergenic for piglets[J].Veterinary Immunology & Immunopathology,2018,202:147-152.

    5. [5]

      WILSON S,BLASCHEK K,DE MEJIA E G.Allergenic proteins in soybean: Processing and reduction of P34 allergenicity[J].Nutrition Reviews,2005,63(2):47-58.

    6. [6]

      付杨,席俊,陈慧彬,等.大豆球蛋白A1a亚基同源建模及抗原表位的预测[J].食品与发酵工业,2022,48(8):22-27.

    7. [7]

      刘阳星月,张迪,姚亚亚,等.大豆过敏原11S球蛋白G2中A2链结合表位的预测[J].食品科学,2018,39(18):152-158.

    8. [8]

      陈慧彬,席俊.大豆球蛋白G5A3亚基多肽链抗原表位的预测及分段克隆载体的构建[J].粮食与油脂,2022,35(10):120-125.

    9. [9]

      王一超,席俊,陈慧彬,等.大豆球蛋白G5A3亚基加工破坏表位的初步定位[J].中国粮油学报,2022,37(10):92-98.

    10. [10]

      梁瑾,王靖飞.B细胞表位预测方法研究进展[J].生命科学,2009,21(2):320-323.

    11. [11]

      HE M X,XI J.Identification of an igE epitope of soybean allergen Gly m Bd 60k[J].LWT-Food and Technology,2020,133:110131.

    12. [12]

      XI J,HE M X.Location of destroyed antigenic sites of Gly m Bd 60k after three processing technologies[J].Food Research International,2020,134:109199.

    13. [13]

      XI J,YAO L L,LI S.Identification of β-conglycinin α' subunit antigenic epitopes destroyed by thermal treatments[J].Food Research International,2020,139:109806.

    14. [14]

      XI J,HE M X,PI J Y.Identification of antigenic sites destructed by high hydrostatic pressure (HHP) of the β subunit of β-conglycinin[J].International Journal of Biological Macromolecules,2019,141:1287-1292.

    15. [15]

      姚利利,席俊,陈慧彬,等.大豆球蛋白兔源多克隆抗血清的制备及其免疫学特性鉴定[J].河南工业大学学报(自然科学版),2020,41(1):13-18.

    16. [16]

      贺梦雪.加工对β-conglycinin抗原性的影响及Gly m Bd 60K破坏表位的定位[D].郑州:河南工业大学,2018.

    17. [17]

      李爽,席俊,贺梦雪,等.大豆致敏原β-伴大豆球蛋白α'亚基的分段与克隆鉴定[J].食品科技,2018,43(3):25-28.

    18. [18]

      XI J,YAO L L,CHEN H B.The effects of thermal treatments on the antigenicity and structural properties of soy bean glycinin[J].Journal of Food Biochemistry,2021,45(9):13874.

    19. [19]

      皮江一.β-conglycinin抗体的制备及加工导致β亚基抗原性降低的亚分子结构定位[D].郑州:河南工业大学,2018.

    20. [20]

      ZHAO J T,CAO Y,ZHANG L.Exploring the computational methods for protein-ligand binding site prediction[J].Computational and Structural Biotechnology Journal,2020,18:417-426.

    21. [21]

      柯丕余.应用蛋白质拉氏图信息提高谷氨酸脱羧酶催化性能的研究[D].杭州:浙江大学,2015.

    22. [22]

      陈慧彬.芝麻主要过敏原Ses i 6抗原表位预测及敏感人群分析[J].江苏调味副食品,2022(2):30-36.

    23. [23]

      闫慧丽.大豆主要过敏原Gly m Bd 28K IgG结合表位的定位研究[D].郑州:河南工业大学,2016.

    24. [24]

      CONG Y J,LI Y,LI L F.Immunoglobulin E and immunoglobulin G cross-reactive allergens and epitopes between cow milk alpha(S1)-casein and soybean proteins[J].Journal of Dairy Science,2020,103(11):9815-9824.

    25. [25]

      GREENBAUM J A,ANDERSEN P H,BLYTHE M,et al.Towards a consensus on datasets and evaluation metrics for developing B-cell epitope prediction tools[J].Journal of Molecular Recognition,2007,20(2):75-82.

Article Metrics

Article views(1886) PDF downloads(15) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return