生物酶在烟草工业中的应用研究进展

黄申; 芦尧; 刘强; 赵声辰; 陈芝飞; 毛多斌

doi:10.12187/2023.05.015

October 2023

Article Contents

HUANG Shen, LU Yao, LIU Qiang, et al. Review on application of biological enzymes in tobacco industry[J]. Journal of Light Industry, 2023, 38(5): 112-118. doi: 10.12187/2023.05.015

Citation: HUANG Shen, LU Yao, LIU Qiang, et al. Review on application of biological enzymes in tobacco industry[J]. Journal of Light Industry, 2023, 38(5): 112-118. doi: 10.12187/2023.05.015 shu

Review on application of biological enzymes in tobacco industry

1. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450002, China

Received Date: 2022-10-17
Accepted Date: 2023-03-27

Abstract

Based on the significant effects of enzyme treatment on tobacco and its products, the research achievements of enzyme treatment in tobacco quality improvement, aroma production, and harm reduction in recent years were reviewed as follows. The weak links and problems in the research of biological enzymes were analyzed. Significant progress had been made in the research of enzymatic degradation of starch and pectin in tobacco leaves, with composite enzymes being more effective than single enzymes. The combination of laccase and protease had a better effect on treating tobacco stems than single. At the same time, treatments with acid, alkali, and steam explosion could significantly improve the enzymatic hydrolysis effect. In the process of reconstituted tobacco leaf concentrate using raw materials for tobacco thin slice preparation, biological enzymes were more effective, with advantages such as short processing time, high efficiency, and significant effects. In terms of aroma production, it was mainly achieved by producing substances that could participate in the Maillard reaction or catalyze the production of aroma precursors. In terms of harm reduction of cigarettes, it was mainly achieved by reducing the content of tobacco specific Nitrosamine and smoke free radicals and other harmful substances. In the future, in-depth research can be carried out from the aspects of structural characterization and characteristics of tobacco biomacromolecules, creation of tobacco enzyme preparations and analysis of catalytic mechanism, so as to further promote the research and application of biological enzymes in tobacco aroma enhancement, quality improvement and harm reduction.
- biological enzymes,
- tobacco industry,
- tobacco leaf treatment,
- tobacco stem treatment
References
1. [1]
  胡飞,段章群,王淮,等.生物酶法制备磷脂酰丝氨酸的研究进展[J].中国油脂,2012,37(6):54-58.
2. [2]
  费璠,刘昌伟,牛丽,等.酶及酶技术在茶叶深加工中的应用[J].食品与机械,2022,38(6):199-204
  ,218.
3. [3]
  叶振敏,戴庆国.酶盐法羊毛纤维低温染色研究[J].化纤与纺织技术,2021,50(5):15-16
  ,89.
4. [4]
  周正红,高孔荣,张水华.烟草中化学成分对卷烟色香味品质的影响及其研究进展[J].烟草科技,1997(2):22-25.
5. [5]
  姜林峰.烟梗中木质素及蛋白质的脱除[D].广州:华南理工大学,2017.
6. [6]
  张晓瑞,刘晓晖,付博,等.烟草中淀粉降解菌的筛选、鉴定及发酵工艺优化[J].食品与机械,2021,37(2):34-41.
7. [7]
  韩迎迎.果胶酶处理烟梗片基的研究[D].广州: 华南理工大学,2014.
8. [8]
  陈兴,王文元,汪显国,等.烟叶纤维素降解菌的筛选、鉴定及其产酶条件优化[J].云南大学学报(自然科学版),2015,37(2):323-328.
9. [9]
  黄申,夏璠,钱玉梅,等.西柏三烯-4,6-二醇降解产香菌培养基条件优化[J].烟草科技,2017,50(12):55-60
  ,69.
10. [10]
  YE J B,ZHENG S S,ZHANG Z,et al. Bacterial cellulose production by Acetobacter xylinum ATCC 23767 using tobacco waste rxtract as culture medium[J].Bioresource Technology,2019(274):518-524.
11. [11]
  王林,朱金峰,许自成,等.不同酶制剂对烤烟上部叶化学成分、游离态和糖苷结合态中性香气成分的影响[J].生物技术进展,2015,5(6):455-460.
12. [12]
  白家峰,梁德全,王宣静,等.酶解工艺及美拉德反应温度优化制备烟草花蕾香料[J].湖北农业科学,2021,60(22):143-147.
13. [13]
  夏炳乐,颜春雷,李敏莉,等.生物酶技术在烟草中的应用研究进展[J].烟草科技,2008(1):46-49.
14. [14]
  樊文举,高娟娟,张建新.外源酶制剂对烤烟烟叶化学品质的影响[J].福建农业学报,2017,32(6):652-659.
15. [15]
  樊文举,高娟娟,张建新.外源酶制剂对烤烟烟叶香气品质及燃烧特性的影响[J].西南农业学报,2018,31(5):948-957.
16. [16]
  雷亚芳. 不同酶制剂与香料处理对烟叶品质及微生物多样性的影响[D].杨凌:西北农林科技大学,2021.
17. [17]
  LIN S S,ZHANG X M,SONG S Q, et al.Tobacco alkaloids reduction by casings added/enzymatic hydrolysis treatments assessed through Plsr analysis[J].Regulatory Toxicology and Pharmacology,2016(75):27-34.
18. [18]
  朱晓兰,刘百战,王维妙,等.利用生物酶降解烟草果胶的研究[J].湖南农业大学学报(自然科学版),2015,41(3):257-262.
19. [19]
  ZHU X L,TAN L L,FENG G L,et al.Research on degradation of cellulose using enzyme treatment in flue-cured tobacco by 13C NMR spectroscopy[J].Cellulose,2015,22(4):2693-2702.
20. [20]
  沙云菲,董惠忠,张耀,等.降解上部烟叶大分子物质的复合酶配制与条件优化[J].中国烟草科学,2018,39(3):80-88
  ,97.
21. [21]
  郑娟娟. 陕西主栽品种烟叶品质特性及酶法调质研究[D].杨凌:西北农林科技大学,2021.
22. [22]
  罗丹,吴俊,全学军,等.淀粉类酶降解烤烟中淀粉的研究[J].重庆理工大学学报(自然科学),2016,30(7):64-68.
23. [23]
  李洪涛,管仕栓,邱承宇,等.利用复合酶制剂改善烟叶枯焦气的研究[J].食品工业,2017,38(12):56-60.
24. [24]
  王宝强,吴潇,季秀玲,等.陈化烟叶产淀粉酶菌株的复筛及产酶条件优化[J].甘肃农业科技,2016(9):13-18.
25. [25]
  贺兆伟,奚家勤,李玉娥,等.产果胶酶疣孢青霉菌株TS63-9发酵条件优化及其在烟草中的应用[J].湖北农业科学,2017,56(4):686-690
  ,696.
26. [26]
  迟广俊,宁伟,徐赫男,等.制丝在线添加复合生物酶对膨胀烟丝感官质量及适用性的影响[J].中国烟草科学,2021,42(2):91-97.
27. [27]
  杨云.漆酶降解烟梗中木质素及其机理研究[D].广州:华南理工大学,2016.
28. [28]
  于建军,魏登辉,田斌强,等.响应面法优化漆酶降解梗丝木质素的工艺[J].烟草科技,2017,50(3):57-64.
29. [29]
  魏登辉. 梗丝木质素降解及其在卷烟工业中的应用[D].郑州:河南农业大学,2017.
30. [30]
  赵梦醒,王燕燕,王昊,等.生物酶预处理对烟梗浆料纤维特性的影响[J].中国造纸,2020,39(7):51-56.
31. [31]
  宋自力,廖头根,张伟,等.白腐真菌血红密孔菌漆酶复合酶预处理烟梗丝的响应面法优化[J].菌物学报,2020,39(5):923-936.
32. [32]
  宋自力,张伟,廖头根,等.血红密孔菌高产漆酶菌株的筛选及其对烟梗的生物降解[J].菌物学报,2019,38(3):381-392.
33. [33]
  ZHU Y,OUYANG X P,ZHAO Y,et al.Oxidative depolymerization of lignin improved by enzymolysis pretreatment with laccase [J]. Journal of Energy Chemistry,2018,27(3):801-805.
34. [34]
  赵梦醒,王燕燕,畅婉清,等.烟梗原料酶促打浆研究[J].天津科技大学学报,2021,36(3):12-17
  ,80.
35. [35]
  徐达,田耀伟,苏加坤,等.不同复合酶在改善梗丝品质中的研究[J].中国酿造,2014,33(11):113-117.
36. [36]
  杨乾栩,和智君,杨蕾,等.复合酶条件优化对烟梗品质提升研究[J].食品与生物技术学报,2016,35(11):1206-1211.
37. [37]
  邹芳,赵娟,雷燕萍,等.一株烟草秸秆降解菌的分离、鉴定及酶学性质研究[J].土壤,2016,48(5):939-945.
38. [38]
  HAN Y Y,LI J,WANG B,et al.Improved enzymatic hydrolysis of tobacco stalk by steam explosion and thread rolling pretreatments[J].Cellulose Chemistry and Technology,2015,49(2):181-185.
39. [39]
  黄天辉,桂金鹏,郑丽沙.利用酶制剂改善再造烟叶品质研究进展[J].东北农业大学学报,2015,46(10):102-108.
40. [40]
  马科,蒋登科,龚霜,等.不同酶处理浓缩液对贵州烟草薄片品质的影响研究[J].轻工学报,2022,37(1):62-67.
41. [41]
  许春平,刘远上,郝辉,等.生物酶法降解烟梗末中果胶的研究[J].食品与生物技术学报,2017,36(2):194-199.
42. [42]
  郝辉,陈芝飞,宋金勇,等.微紫青霉(Penicillium janthinellum sw 09)发酵产果胶酶降解烟梗果胶的条件优化及产物分析[J].西南农业学报,2015,28(6):2756-2762.
43. [43]
  CHEN C,JIANG L F,MA G F,et al.Lignin removal from tobacco stem with laccase improved by synergistic action of weak alkali and tween 80[J].Waste and Biomass Valorization,2019,10(11):3343-3350.
44. [44]
  徐菲菲,田海龙,李东亮,等.复合酶法降低烟草薄片刺激性的研究[J].江苏农业科学,2020,48(14):227-233.
45. [45]
  王茜茜,刘恩芬,陈正春,等.应用生物酶提高造纸法再造烟叶品质的研究[J].纸和造纸,2020,39(6):15-20.
46. [46]
  刘恩芬,赵晓晨,王军,等.造纸法再造烟叶生产过程中梗膏提质酶技术的应用研究[J].纸和造纸,2020,39(3):27-31.
47. [47]
  刘彩红. 烟草废弃物酶解与Maillard反应及在烟草加香中的研究[D].郑州:郑州大学,2016.
48. [48]
  许春平,杨琛琛,王铮,等.复合生物酶液处理低次烟叶制备烟用香料[J].郑州轻工业学院学报(自然科学版),2014,29(2):23-26.
49. [49]
  刘珊,胡军,胡有持,等.黄嘌呤氧化酶催化氧化降解β-胡萝卜素[J].烟草科技,2014(10):52-55.
50. [50]
  刘兴余,张杰,白若石,等. 生物酶选择性降低NNK研究[C]//上海市烟草学会.上海市烟草学会2015年度获奖论文专刊.上海:[出版社不详],2016:44-48.
51. [51]
  LIU L Z,YU W,LUO D,et al.Catalase nanocapsules protected by polymer shells for scavenging free radicals of tobacco smoke[J].Advanced Functional Materials,2015,25(32):5159-5165.
52. [52]
  DONG C W,ZHENG Y D,TANG H Z H,et al.Highly efficient synthesis of 2,5-dihydroxypyridine using Pseudomonas sp. ZZ-5 nicotine hydroxylase immobilized on immobead 150[J].Catalysts,2018,8(11):548.
53. [53]
  ZHENG Y X,WANG Y L,PAN J,et al.Semi-continuous production of high-activity pectinases by immobilized rhizopus oryzae using tobacco wastewater as substrate and their utilization in the hydrolysis of pectin-containing lignocellulosic biomass at high solid content [J].Bioresource Technology,2017(241):1138-1144.
54. [54]
  ZHANG G,LI S G,XU Y B,et al.Production of alkaline pectinase:A case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis cas-mei-2-33[J].BMC Biotechnology,2019,19(1):1-11.
Proportional views

Get Citation

PDF

XML

Article Metrics

Article views(2467) PDF downloads(66) Cited by()

Ralated

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Review on application of biological enzymes in tobacco industry

Abstract

References

Proportional views

Article Metrics

Ralated

Proportional views

通讯作者: 陈斌, bchen63@163.com