结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展

赵泽鑫; 周灵; 韩美玲; 许源; 蔡俊

doi:10.12187/2024.01.001

February 2024

Article Contents

ZHAO Zexin, ZHOU Ling, HAN Meiling, et al. Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering[J]. Journal of Light Industry, 2024, 39(1): 1-11. doi: 10.12187/2024.01.001

Citation: ZHAO Zexin, ZHOU Ling, HAN Meiling, et al. Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering[J]. Journal of Light Industry, 2024, 39(1): 1-11. doi: 10.12187/2024.01.001 shu

Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering

School of Bioengineering and Food science/Key Laboratory of Fermentation Engineering/Hubei Collaborative Innovation Center of Industrial Fermentation/Hubei Key Laboratory of Industrial Mincrobiology, Hubei University of Technology, Wuhan 430068, China

Received Date: 2023-08-26
Accepted Date: 2023-10-12

Abstract

The utilization of lipase in synthesis structured lipids (SLs) from natural oils confers additional health properties and effectively enhances their value-added potential. This paper provided a comprehensive review of recent studies on the enzymatic preparation of SLs, summarizing the advancements in structural design and immobilized enzyme engineering technology to improve the efficiency of their synthesis by enzymatic approaches. The sn-1,3 regiospecific lipase enabled the precise synthesis of SLs, while employing stepwise variable temperature reactions, supercritical fluid reactions, vacuum reactions, and ultrasonic-assisted methods could inhibit the acyl migration effects and further enhance product yield. Furthermore, structural design could enhance the catalytic efficiency, stability, and substrate specificity of lipases to elevate the efficiency and yield in enzymatic synthesis of SLs. Immobilized enzyme technology could enhance enzyme reactivity and stability while reducing the costs of enzymatic synthesis of SLs. In the future, the high-performance enzymes should be developed by combining structural modification and novel immobilization technology on the basis of the clearer understanding of their structure-function characteristics. These insights would contribute to innovation and advancement in efficient and precise enzymatic synthesis processes for SLs.
- structured lipids,
- lipase,
- enzymatic synthesis,
- enzyme engineering,
- structure design,
- immobilization
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沈阳化工大学材料科学与工程学院沈阳 110142

Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering

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通讯作者: 陈斌, bchen63@163.com