云产雪茄烟叶微生物群落、游离氨基酸及挥发性风味物质特征分析

王海清; 郭东锋; 丁乃红; 张明珠; 时雅琪; 吴学凤; 穆冬冬; 李兴江

doi:10.12187/2024.04.013

August 2024

Article Contents

WANG Haiqing, GUO Dongfeng, DING Naihong, et al. Characterization of microbial communities, free amino acids and volatile flavor compounds in cigar tobacco leaves from Yunnan province[J]. Journal of Light Industry, 2024, 39(4): 97-108. doi: 10.12187/2024.04.013

Citation: WANG Haiqing, GUO Dongfeng, DING Naihong, et al. Characterization of microbial communities, free amino acids and volatile flavor compounds in cigar tobacco leaves from Yunnan province[J]. Journal of Light Industry, 2024, 39(4): 97-108. doi: 10.12187/2024.04.013 shu

Characterization of microbial communities, free amino acids and volatile flavor compounds in cigar tobacco leaves from Yunnan province

1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China;
2. Technology Center, China Tobacco Anhui Industrial Co., Ltd., Hefei 230009, China

Received Date: 2023-12-19
Accepted Date: 2024-03-01
Available Online: 2024-08-15

Abstract

In order to investigate the quality differences of cigar tobacco leaves from Yunnan production areas, microbial community structure, free amino acids and volatile flavor compounds of cigar tobacco leaves produced in Yunnan province were analyzed by high-throughput sequencing and metabolomics. O2PLS analysis and correlation analysis were used to systematically reveal the relationship between microorganisms of origin and tobacco flavor quality, and to identify functional microorganisms and synergistic taxa in tobacco. The results showed that there were differences in the microbial community structure of cigar tobacco leaves produced in Yunnan province, in which the dominant bacteria were mainly Aerococcus, Staphylococcus and Corynebacterium, and the dominant fungi were mainly Aspergillus, Cladosporium and Alternaria. 17 free amino acids were detected, and the overall amino acid content showed that Asp was the highest and Met was the lowest, with higher TAV Asp and Glu contributed more to its flavor presentation. The free amino acid flavor profiles of Lincang Yunxue No.2 and Pu'er Yunxue No.2 cigar tobacco leaves was more prominent. A total of 65 volatile flavor compounds were detected, mainly ketones, among which 16 different flavor compounds, including 6-Methyl-5-hepten-2-one, β-Damascone, Phenylethyl alcohol, Decanal, and Dihydroactinidiolide, played an important role in the formation of its flavor. The overall flavor characteristics of Pu'er No.2 were more prominent. Sphingomonas, Staphylococcus, Pseudomonas, and Oceanobacillus acted synergistically to contribute to cigar tobacco leaves flavor formation, while the relative abundance of Penicillium was negatively correlated with tobacco quality.
- cigar tobacco leaf,
- bacteria,
- fungi,
- volatile flavor compound,
- free amino acid
References
1. [1]
  KRVSEMANN E J Z,VISSER W F,CREMERS J W J M,et al.Identification of flavour additives in tobacco products to develop a flavour library[J].Tobacco Control,2018,27(1):105-111.
2. [2]
  李秀妮,闫铁军,吴风光,等.全球主要产地雪茄烟叶的风味特征初探[J].中国烟草学报,2019,25(6):126-132.
3. [3]
  XIA B,FENG M M,XU G,et al.Investigation of the chemical compositions in tobacco of different origins and maturities at harvest by GC-MS and HPLC-PDA-QTOF-MS[J].Journal of Agricultural and Food Chemistry,2014,62(22):4979-4987.
4. [4]
  XIE B,JONES P,DWIVEDI R,et al.Evaluation,comparison,and unique features of ecological security in southwest China:A case study of Yunnan Province [J].Ecological Indicators,2023,153:110453.
5. [5]
  ZHENG T F,ZHANG Q Y,LI P H,et al.Analysis of microbial community,volatile flavor compounds,and flavor of cigar tobacco leaves from different regions[J].Frontiers in Microbiology,2022,13:907270.
6. [6]
  贾云,胡婉蓉,吕晋雄,等.雪茄烟叶发酵过程中微生物群落及功能微生物分析[J].轻工学报,2023,38(1):71-78
  ,89.
7. [7]
  ZHANG M Z,GUO D F,WANG H Q,et al.Analyzing microbial community and volatile compound profiles in the fermentation of cigar tobacco leaves[J]. Applied Microbiology and Biotechnology,2024,108(1):243.
8. [8]
  赵晓超,李林林,张贝贝,等.地衣芽孢杆菌FZ1对发酵雪茄烟叶中性致香成分的影响[J].贵州农业科学,2023,51(10):123-132.
9. [9]
  郑天飞,张倩颖,李东亮,等.不同产地雪茄烟叶的风味特征和微生物群落分析[J].河南农业科学,2022,51(1):45-54.
10. [10]
  宋学茹,张立猛,卜令铎,等.云南不同产地醇化雪茄烟叶微生物群落差异研究[J].中国烟草科学,2022,43(5):87-93.
11. [11]
  吴丽君,张鸽,陈晓娜,等.云南雪茄烟叶人工发酵过程中化学成分与细菌群落变化及其相关性分析[J].中国烟草学报,2023,29(4):124-134.
12. [12]
  王硕立,丁松爽,王荣浩,等.我国云南和尼加拉瓜雪茄烟叶矿质元素含量和感官质量差异及其相关分析[J].作物杂志,2023(3):139-147.
13. [13]
  ZHAO Y Z,LIU S P,HAN X,et al.Combined effects of fermentation temperature and Saccharomyces cerevisiae strains on free amino acids,flavor substances, and undesirable secondary metabolites in huangjiu fermentation[J].Food Microbiology,2022,108:104091.
14. [14]
  程昌合,解莹莹,王雪丽,等.不同香型烤烟游离氨基酸组成和含量的差异分析[J].江西农业学报,2014,26(5):84-86
  ,90.
15. [15]
  沈晗,葛炯,王逸枫,等.福建烟叶游离氨基酸等主要含氮化合物与感官质量的关系[J].中国烟草学报,2022,28(5):23-31.
16. [16]
  赵方杰,廉喜红,胡小平,等.不同产地西洋参氨基酸种类及含量分析[J].西北农业学报,2020,29(7):1051-1058.
17. [17]
  潘勇,吴巧茵,李林林,等.国产雪茄烟叶堆积发酵过程中的微生物变化规律及差异分析[J].河南农业科学,2022,51(11):171-180.
18. [18]
  DI GIACOMO M,PAOLINO M,SILVESTRO D,et al.Microbial community structure and dynamics of dark fire-cured tobacco fermentation[J].Applied and Environmental Microbiology,2007,73(3):825-837.
19. [19]
  HU Y Y,CHEN Q,WEN R X,et al.Quality characteristics and flavor profile of Harbin dry sausages inoculated with lactic acid bacteria and Staphylococcus xylosus[J].LWT-Food Science and Technology,2019,114:108392.
20. [20]
  黄贺敏,吴丽香,邓梅忠,等.不同品质烟叶微生物群落与其挥发性成分的关联研究[J].食品与生物技术学报,2022,41(12):85-95.
21. [21]
  周家喜,喻理飞,张晓敏,等.仓储烟叶表面真菌群落组成分析[J].菌物学报,2018,37(4):434-443.
22. [22]
  寇明钰,汪长国,戴亚,等.不同产地和等级的雪茄烟叶中游离氨基酸含量分析[J].西南农业学报,2013,26(3):963-967.
23. [23]
  李晶晶,史宏志,杨兴有,等.采收时期及调制方式对万源晒烟调制前后氨基酸含量变化的影响[J].中国烟草学报,2018,24(1):68-76.
24. [24]
  贾国涛,王晓瑜,孙溢明,等.我国八大香型生态区域烤烟游离氨基酸含量与烟叶品质的关系分析[J].作物杂志,2023(3):195-199.
25. [25]
  胡婉蓉,蔡文,李东亮,等.发酵介质对雪茄烟叶化学成分及微生物群落结构的影响[J].轻工学报,2023,38(1):90-100.
26. [26]
  SHEN H,HUANG M Q,ZHAO M M,et al.Interactions of selected ketone flavours with porcine myofibrillar proteins:The role of molecular structure of flavour compounds[J].Food Chemistry,2019,298:125060.
27. [27]
  张梦玥,史宏志,毕艳玖,等.白肋烟、晒烟和烤烟烟叶在6年贮藏过程中主要酮类香气成分的变化趋势[J].中国烟草学报,2018,24(5):23-34.
28. [28]
  PIORNOS J A,BALAGIANNIS D P,METHVEN L,et al.Elucidating the odor-active aroma compounds in alcohol-free beer and their contribution to the worty flavor[J].Journal of Agricultural and Food Chemistry,2020,68(37):10088-10096.
29. [29]
  贺佩,王以慧,耿召良,等.不同产区雪茄茄芯烟叶关键挥发性香气成分分析[J].中国烟草科学,2023,44(1):92-99.
30. [30]
  XU Y Q,ZHAO J R,LIU X,et al.Flavor mystery of Chinese traditional fermented Baijiu:The great contribution of ester compounds [J].Food Chemistry,2022,369:130920.
31. [31]
  靖军领,黄本荣,林伟杰,等.不同干筋温度对翠碧1号烟叶烘烤质量的影响[J].安徽农业科学,2017,45(13):80-84.
32. [32]
  杨淳婷,张梦玥,赵园园,等.低烟碱品种及栽培模式对烤烟化学成分及感官质量的影响[J].中国烟草学报,2022,28(6):85-93.
33. [33]
  孟凡来,赵昶灵,段丽斌,等.高等植物类胡萝卜素的生物降解途径研究进展[J].中国农学通报,2013,29(24):143-150.
34. [34]
  张鸽,梁开朝,辛玉华,等.基于高通量测序和传统分离研究雪茄外包皮表面细菌多样性及演替[J].应用与环境生物学报,2018,24(4):783-788.
35. [35]
  张鸽,李志豪,邓帅军,等.海南H382雪茄烟叶不同发酵周期细菌群落多样性表征及演替分析[J].中国烟草学报,2021,27(2):117-126.
36. [36]
  冯颖杰,袁岐山,杨宗灿,等.一株鞘氨醇单胞菌对复烤后烟叶多酚物质的降解作用[J].中国烟草学报,2019,25(1):19-24.
37. [37]
  邢家爱,于庆泉,李福东,等.混菌发酵对西拉葡萄酒香气品质的影响[J].食品研究与开发,2023,44(20):76-82.
38. [38]
  ZHOU J X,CHENG Y,YU L F,et al.Characteristics of fungal communities and the sources of mold contamination in mildewed tobacco leaves stored under different climatic conditions[J].Applied Microbiology and Biotechnology,2022,106(1):131-144.
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Characterization of microbial communities, free amino acids and volatile flavor compounds in cigar tobacco leaves from Yunnan province

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