不同马尾松松针提取液的成分分析与抗氧化活性研究

刘亚群; 陈良辉; 张泽坤; 郑玉忠; 张振霞

doi:10.12187/2025.02.007

April 2025

Article Contents

LIU Yaqun, CHEN Lianghui, ZHANG Zekun, et al. Analysis and antioxidant activity study of different extraction liquid from Pinus massoniana needles[J]. Journal of Light Industry, 2025, 40(2): 61-71. doi: 10.12187/2025.02.007

Citation: LIU Yaqun, CHEN Lianghui, ZHANG Zekun, et al. Analysis and antioxidant activity study of different extraction liquid from Pinus massoniana needles[J]. Journal of Light Industry, 2025, 40(2): 61-71. doi: 10.12187/2025.02.007 shu

Analysis and antioxidant activity study of different extraction liquid from Pinus massoniana needles

LIU Yaqun ^1,2 ,
CHEN Lianghui ^1,2 ,
ZHANG Zekun ^1,2 ,
ZHENG Yuzhong ^1,2 ,
ZHANG Zhenxia ^1,2,,

1. School of Life Sciences and Food Technology, Hanshan Normal University, Chaozhou 521041, China;
2. Guangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Chaozhou 521041, China

Corresponding author: ZHANG Zhenxia, zzx8411@hstc.edu.cn
Received Date: 2024-04-12
Accepted Date: 2024-07-22

Abstract

To analyze the chemical composition and antioxidant activity of Pinus massoniana needles, the main components of essential oils, aqueous extracts, and alcoholic extracts were studied using gas chromatography-mass spectrometry (GC-MS) coupling method. Additionally, network pharmacology was utilized to investigate the active components, and free radical scavenging experiments were conducted to evaluate their antioxidant activities. The results revealed that 60 compounds, accounting for 95.13% of the total, were identified in the essential oils, primarily consisting of terpenes. The aqueous and alcoholic extracts contained 10 and 16 compounds, respectively, mainly consisting of ketones. Significant differences were observed in the composition distribution among different extraction methods. VIP analysis identified six potential candidate differential compounds: pinene (VIP=1.31), 1-caryophyllene (VIP=1.47), L-β-pinene (VIP=1.80), α-pinene (VIP=3.32), 3-nitro-1-phenylpropyl-1-ketone (VIP=7.86), and 2-ethoxyphenyl isothiocyanate (VIP=1.13). These compounds may exert effects on oxidative stress and free radical scavenging pathways through interactions with proteins such as aldehyde reductase (AR). In the evaluation of antioxidant activity, when the DPPH scavenging rate reached 80%, the mass concentrations of alcoholic extract, aqueous extract, and essential oil were 0.2 mg/mL, 0.9 mg/mL, and 80 mg/mL, respectively. When the ABTS⁺ scavenging rate reached 80%, the concentrations of alcoholic extract, aqueous extract, and essential oil were 0.07 mg/mL, 0.2 mg/mL, and 10 mg/mL, respectively. When the hydroxyl radical scavenging rate reached 80%, the concentration of alcoholic extract was 3.5 mg/mL, while the scavenging abilities of aqueous extract and essential oil were relatively low and unstable. At the mass concentration of the extract liquid was 1.00 mg/mL, the alcoholic extract exhibited the strongest total reducing power.Therefore, Pinus massoniana needles possess significant antioxidant potential, with various components exhibiting antioxidant activities obtainable through different extraction methods.
- Pinus massoniana needle,
- extraction method,
- gas chromatography-mass spectrometry(GC-MS) coupling method,
- network pharmacology,
- antioxidant activity
References
1. [1]
  桂琴.马尾松林下套种药材的适宜性及经济效益分析:以5种药用植物为例[D].贵阳:贵州大学,2023.
2. [2]
  张彩珍.广西地区马尾松种植技术与经济效益浅析[J].南方农业,2020,14(3):9-10.
3. [3]
  李萍,万德光,刘玮琦,等.马尾松针叶中挥发油化学成分分析[J].中国中药杂志,2003,28(2):180.
4. [4]
  LIU H Y,CHEN H Y,DING G J,et al.Identification of candidate genes conferring tolerance to aluminum stress in Pinus massoniana inoculated with ectomycorrhizal fungus[J].BMC Plant Biology,2020,20(1):521.
5. [5]
  SHANG H Q,NIU X Y,CUI W P,et al.Anti-tumor activity of polysaccharides extracted from Pinus massoniana pollen in colorectal cancer-in vitro and in vivo studies[J].Food & Function,2022,13(11):6350-6361.
6. [6]
  QI J S,PING D B,SUN X,et al.A herbal product inhibits carbon tetrachloride-induced liver fibrosis by suppressing the epidermal growth factor receptor signaling pathway[J].Journal of Ethnopharmacology,2023,311:116419.
7. [7]
  傅钰,史璇,张道明,等.低共熔溶剂提取马尾松松针抗氧化成分的研究[J].北京林业大学学报,2021,43(7):149-158.
8. [8]
  潘炘,陈顺伟,庄晓伟.不同提取工艺的马尾松松针提取物抗氧化性能研究[J].生物质化学工程,2009,43(3):21-24.
9. [9]
  聂瑞馨,李涛,陈晶晶,等.马尾松松针主要活性成分提取及应用研究进展[J].农产品加工,2020(5):79-81,84.
10. [10]
  孟亚会,闵建华,尚强.马尾松松针化学成分分析[J].亚太传统医药,2021,17(3):60-62.
11. [11]
  罗晓蔓,邴靖楠,马晓鹏.马尾松苗木根际土壤浸提物的GC-MS分析[J].绿色科技,2023,25(21):121-124
  ,130.
12. [12]
  粟本超,谢济运,陈小鹏,等.广西柳州产马尾松和湿地松松针挥发油的GC/MS分析[J].质谱学报,2008,29(2):70-75.
13. [13]
  李成成,苑楠楠,白桦芳,等.基于HPLC指纹图谱和网络药理学的消炎退热颗粒质量标志物(Q-Marker)预测[J].中草药,2021,52(13):3885-3895.
14. [14]
  吴媛媛,田萌,谢锋,等.TRPV1参与心肌缺血/再灌注损伤保护作用机制研究进展[J].辽宁中医药大学学报,2020,22(2):67-71.
15. [15]
  李炜娟,肖元梅.PARP1在氧化应激诱导的DNA损伤及其修复过程中的作用[J].中国老年学杂志,2015,35(23):6924-6925.
16. [16]
  JIANG Z Q,GAO W,HUANG L Q.Tanshinones,critical pharmacological components in Salvia miltiorrhiza[J].Frontiers in Pharmacology,2019,10:202.
17. [17]
  HUMPTON T J,HOCK A K,MADDOCKS O D K,et al.p53-mediated adaptation to serine starvation is retained by a common tumour-derived mutant[J].Cancer & Metabolism,2018,6:18.
18. [18]
  许晖,孙兰萍,张斌,等.微生物阿魏酸酯酶的研究进展[J].中国酿造,2008,27(10):11-16.
19. [19]
  董毅.自由基在缺氧引起突触前谷氨酸释放增加中的作用和机制[D].上海:复旦大学,2010.
20. [20]
  孙臣友,方周溪,戚双双,等.莪素油对慢性低氧大鼠学习与记忆和海马p-CaMKII表达的影响[J].中国病理生理杂志,2008,24(11):2156-2161.
21. [21]
  王冬艳,华欣,叶记林,等.江苏地产白首乌C₂₁甾体苷对高血脂大鼠的肝脏保护作用和抗氧化作用研究[J].河北医药,2016,38(3):335-337.
22. [22]
  胡文杰,李阁,李冠喜.马尾松松针挥发油化学成分及抗氧化活性研究[J].中国粮油学报,2018,33(12):42-48.
23. [23]
24. [24]
25. [25]
  董银卯,邓小锋.化妆品植物原料现状、应用与发展趋势[J].轻工学报,2016,31(04):30-38.
26. [26]
  王雅琪,杨园珍,伍振峰,等.中药挥发油传统功效与现代研究进展[J].中草药,2018,49(2):455-461.
27. [27]
  田民义.姜科挥发油及其萜类成分抗肿瘤活性及相关机制研究[D].贵阳:贵州大学,2021.
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Analysis and antioxidant activity study of different extraction liquid from Pinus massoniana needles

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